3-18
Troubleshooting, Testing, and Maintenance
Reproducibility errors
Solvent not properly
degassed or sparged
Degas or sparge
solvent.
Incorrect chemistry
Check chemistry.
Incorrect integration
Incorrect integration.
Injector problem
Troubleshoot injector.
Baseline drift, rapid
Column not
equilibrated
Equilibrate column.
Detector not allowed to
warm up
Allow detector to warm
up until baseline is
stable (time varies
based on wavelength
and sensitivity).
Solvent contaminated
Use fresh solvent.
Solvent not properly
degassed (rapid or slow
drift)
Degas solvent.
Flow fluctuations (rapid
or slow drift
Troubleshoot fluid
handling unit.
Incorrect wavelength
for solvent
Use correct wavelength
and solvent (make sure
solvent does not have a
UV absorbance at the
wavelength used).
Chromatography troubleshooting (Continued)
Symptom
Possible cause
Corrective action
Summary of Contents for HPLC
Page 34: ...1 24 Overview...
Page 84: ...2 50 Installing the System...
Page 130: ...3 46 Troubleshooting Testing and Maintenance Test results with all valves working...
Page 174: ...A 14 Safety Advisories...
Page 190: ...D 10 Solvent Considerations...
Page 198: ...Index 8 Waters Technical Service contacting 3 2 Workstations Empower 2 15 MassLynx 2 15...