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Waters 996, Operator'S Manual

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Waters 996 Operator'S Manual Download Page 31

PDA Calibration

31

2

You can run all user-initiated diagnostics from QuickSet in the Millennium

32

 software. For 

more information on QuickSet and PDA diagnostics, refer to the “PDA Diagnostics 
Window” topic in the 

Millennium

32

 Online Help 

Find tab.

If you encounter a problem that you cannot troubleshoot (see 

Section 2.1, Startup 

Diagnostics

), contact Waters Technical Service at (800) 252-4752, 

U.S. and Canadian 

customers only

. Other customers, call your local Waters subsidiary or your local Waters 

Technical Service Representative, or call Waters corporate headquarters for assistance at 
1-508-478-2000 (U.S.). 

2.3  PDA Calibration 

You can adjust, or calibrate, the 996 detector to ensure that wavelength readings are 
accurate. Recalibrate the 996 detector 

only

 if the Wavelength Accuracy diagnostic (in the 

Internal Diagnostics tests) fails. 

You calibrate the 996 detector using the PDA Calibration window, which you access from 
QuickSet and which allows you to:

• View the effects of exposure time on photodiode saturation for a given wavelength 

range. 

• Verify the wavelength location of the deuterium spectrum Balmer lines (486.0 nm 

and
656.1 nm).

• Recalibrate to set the 486-nm peak at the proper wavelength.

• Ensure precise data for library matching.

Note:

 The system administrator can restrict access to the PDA Calibration window. 

Recalibrating the wavelength requires that spectral libraries be reentered.

Required Materials

• HPLC-grade methanol

• HPLC-grade water

Preparing for Calibration

Note:

 Ensure that the flow cell is clean before you check calibration. (See 

Section 3.1.1, 

Flushing the Flow Cell

.)

Summary of Contents for 996

Page 1: ...Waters 996 PDA Detector Operator s Guide 34 Maple Street Milford MA 01757 053021TP Revision 0 ...

Page 2: ...N PRINTED IN THE UNITED STATES OF AMERICA ALL RIGHTS RESERVED THIS BOOK OR PARTS THEREOF MAY NOT BE REPRODUCED IN ANY FORM WITHOUT THE WRITTEN PERMISSION OF THE PUBLISHER Millennium PowerStation and Waters are registered trademarks and busLAC E is a trademark of Waters Corporation All other trademarks are the sole property of their respective owners The quality management system of Waters chromato...

Page 3: ...ttention This equipment has been tested and found to comply with the limits for a Class A digital device pursuant to Part 15 of the FCC rules These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordanc...

Page 4: ...uits radioélectriques dépassant les limites applicables aux appareils numériques de la classe A prescrites dans les règlements sur le brouillage radioélectrique édictés par le Ministère des Communications du Canada Symbols Used on the Waters 996 Photodiode Array Detector Direct current Alternating current Protective conductor terminal Frame or chassis terminal Caution risk of electric shock high v...

Page 5: ... Connecting the IEEE 488 Cable 16 1 3 2 Setting the IEEE 488 Address 18 1 4 Non IEEE 488 Communication Connections 19 1 4 1 Connecting Analog Output Cables 19 1 4 2 Connecting Event Cables 20 1 5 Fluidic Connections 22 1 6 Startup Shutdown 24 Chapter 2 Diagnostics and Calibration 27 2 1 Startup Diagnostics 27 2 2 User Initiated Diagnostics 30 2 3 PDA Calibration 31 Table of Contents ...

Page 6: ... 41 3 3 Replacing the Fuses 43 Chapter 4 Principles of the 996 PDA Detector Optics 44 4 1 996 Detector Optics 44 4 2 Resolving Spectral Data 46 4 3 Measuring Light at the Photodiode 47 4 4 Computing Absorbance Data Points 50 4 4 1 Calculating Absorbance 50 4 4 2 Resolution 52 4 4 3 Filtering Data 53 Chapter 5 Spectral Contrast Theory 54 5 1 Comparing Absorbance Spectra 54 5 2 Representing Spectra ...

Page 7: ...5 4 2 Photometric Error 61 5 4 3 Solvent Changes 61 5 4 4 Threshold Angle 61 Appendix A Detector Specifications 63 Appendix B Spare Parts 64 Appendix C Warranty Information 65 C 1 Limited Express Warranty 65 C 2 Shipments Damages Claims and Returns 69 Appendix D Mobile Phase Absorbance 70 Index 74 ...

Page 8: ...s 36 3 4 Disassembling the Flow Cell 37 3 5 Lamp Access Door 40 3 6 Lamp Usage Indicator 40 3 7 Lamp Power Cord and Mounting Screws 42 3 8 Fuse Block 43 4 1 Optics Assembly Light Path 45 4 2 Benzene Spectrum at 1 2 nm Resolution 47 4 3 Photodiodes Discharged by Light 48 4 4 Absorbance as a Function of Concentration 51 5 1 Comparing Spectra of Two Compounds 55 5 2 Plotting Vectors for Two Spectra 5...

Page 9: ...or Switch Closure 21 1 3 Event Out Terminal Specifications on Contact Closure 21 2 1 996 Detector Troubleshooting 28 4 1 Optics Assembly Components 45 A 1 996 Detector Specifications 63 B 1 Spare Parts 64 C 1 Warranty Periods 68 D 1 Mobile Phase Absorbance Measured Against Air or Water 70 List of Tables ...

Page 10: ... of PDA detector data by Millennium 32 software Structure of This Guide The Waters 996 PDA Detector Operator s Guide is divided into chapters and appendixes Each chapter and appendix page is marked with a tab and a footer to help you quickly access information The following table describes the material covered in each chapter and appendix of this guide Chapter Appendix Description Chapter 1 Instal...

Page 11: ...elp Describes all Millennium32 windows menus menu selections and dialog boxes Also includes reference information and procedures for performing all tasks required to use the Millennium32 software Included as part of the Millennium32 software Millennium32 Software Getting Started Guide Provides an introduction to the Millennium 32 System Describes the basics of how to use Millennium 32 software to ...

Page 12: ...ons Used in This Guide This guide uses the following conventions to make text easier to understand Purple Text indicates user action For example Press 0 then press Enter for the remaining fields Italic text denotes new or important words and is also used for emphasis For example An instrument method tells the software how to acquire data Millennium32 System Installation Configuration Guide Describ...

Page 13: ...ocated to the front of the lamp retainer as described in Section 3 2 1 Checking Lamp Usage Notes Attentions and Cautions Notes call out information that is important to the operator For example Note Record your results before you proceed to the next step Attentions provide information about preventing possible damage to the system or equipment For example Attention To avoid damaging the detector f...

Page 14: ...struments that are not compatible with Millennium software control 1 1 Installation Site Requirements Install the Waters 996 PDA Detector Figure 1 1 at a site that meets the specifications listed in Table 1 1 Table 1 1 Site Requirements Factor Specification Ambient temperature 4 to 40 C 39 to 104 F Relative humidity 20 to 80 percent noncondensing Bench space Width 11 5 in 29 cm Depth 24 in 61 cm H...

Page 15: ...ements for the Waters 996 PDA Detector are Voltage range 95 to 240 Vac 10 Frequency range 50 to 60 Hz 3 Hz Fuses The Waters 996 PDA Detector is shipped with fuses rated for North American operation If you operate the Waters 996 PDA Detector in another location install the IEC rated fuses supplied in the Waters 996 Detector Startup Kit in the fuse holder in the rear of the detector refer to Section...

Page 16: ...EEE 488 bus All detector control and data acquisition communications take place over the IEEE 488 bus Note If an inject start signal is not available over the IEEE 488 bus you must provide a signal at the Event In terminals on the 996 detector rear panel see Section 1 4 2 Connecting Event Cables 1 3 1 Connecting the IEEE 488 Cable Note If the 996 detector is to be rack mounted or stacked on other ...

Page 17: ... can connect the 2690 separations module before or after the 996 detector 2 Use another IEEE 488 cable to connect to the stackable connector on the first instrument and the IEEE 488 connector on another instrument 3 Repeat step 2 for each IEEE 488 instrument in your chromatographic system up to a maximum of 14 IEEE 488 instruments Note Keep in mind cable length limitations when you set up your sys...

Page 18: ...from 2 to 29 and must be unique within your network Refer to the Millennium 32 System Installation Configuration Guide Section 2 3 1 Connecting IEEE 488 Devices for the correct IEEE 488 DIP switch settings Figure 1 4 Locating the IEEE 488 Address Switches 2 To instruct the 996 detector to accept the new IEEE 488 address power off then power on the detector see Section 1 6 Startup Shutdown IEE 488 ...

Page 19: ...o not use cables longer than 9 8 feet 3 meters when you make connections to the screw type barrier terminal strips In addition ensure that you always connect the shield of each cable to chassis ground at one instrument only 1 4 1 Connecting Analog Output Cables The values of the analog output signals generated by the 996 detector are specified by parameter values set from the Millennium 32 Worksta...

Page 20: ... 4 Reinstall the Analog Output strip 1 4 2 Connecting Event Cables The 996 detector has four terminal strip connections for contact closure signals Two input inject start signal terminals Two output programmable event table signal terminals If an inject start signal is not available over the IEEE 488 bus you must provide a signal at an Event In terminal on the 996 detector rear panel Manual inject...

Page 21: ...d Table 1 3 Attention To avoid damage to the 996 detector electronics be sure you make the proper electrical connections as outlined in this section Required Materials Small flat blade screwdriver One electrical insulation stripping tool Event signal cables in Startup Kit Table 1 2 Event In Inject Start Terminal Specifications on TTL or Switch Closure Parameter Specification Low trigger 1 8 V High...

Page 22: ... appropriate event input or output terminal see Figure 1 6 Tighten the two screws to secure the and wires 3 Connect the other end of the event signal cable to the appropriate event input or event output terminal on the external device 4 Reinstall the Event Input Output strip 1 5 Fluidic Connections Caution To avoid chemical hazards always observe safe laboratory practices when handing solvents Ref...

Page 23: ...llection bottle Note Ensure the length of this tubing is at least 1 to 2 feet 30 to 60 cm to prevent air bubbles from forming in the flow cell 2 Cut the two lengths of tubing as follows Use a Waters 1 16 inch stainless steel tubing cutter or a file with a cutting edge to scribe the circumference of the tubing at the desired break point Grasp the tubing on both sides of the scribed mark with cloth ...

Page 24: ...ger tight Insert the other end of the tubing in the waste container Attention To avoid damage to the flow cell avoid pressures approaching the maximum pressure for the analytical flow cell 1000 psi 70 kg cm2 1 6 Startup Shutdown Follow the procedures in this section to ensure reliable detector performance Startup To start up the 996 detector 1 In your instrument method set the solvent delivery sys...

Page 25: ...8 to the 1 On position 4 Observe the Lamp and Status indicator lights on the front panel of the detector Figure 1 8 If both lights remain illuminated the detector passed the internal diagnostics If either indicator light blinks or is off refer to the troubleshooting tables in Chapter 2 Diagnostics and Calibration Figure 1 8 996 Detector Indicator Lights 5 Wait 1 hour for the deuterium lamp to stab...

Page 26: ...fers set the solvent delivery system or pump to deliver 1 mL min of HPLC grade water for 10 minutes Otherwise set the solvent delivery system or pump to deliver 1 mL min of degassed methanol for 10 minutes 2 Press the 0 1 Off On Switch on the front panel of the detector to the 0 Off position ...

Page 27: ... Workstation Additional information about the performance of the detector is also available through the PDA Calibration window accessed from QuickSet in the Millennium32 software If you encounter a problem that you cannot troubleshoot see Section 2 1 Startup Diagnostics contact Waters Technical Service at 800 252 4752 U S and Canadian customers only Other customers call your local Waters subsidiar...

Page 28: ...or power Blown fuse Replace fuse see Section 3 3 Replacing the Fuses Detector is still performing diagnostics Wait for diagnostics to end Status light blinks and lamp light off Failed startup diagnostics 1 Check that lamp door is secure 2 Replace lamp If replacing the lamp fails to correct the problem contact Waters Technical Service TP01460 LAMP WATERS 996 Photodiode Array Detector On Off Switch ...

Page 29: ...lure message Shutter failure Run the Shutter diagnostic For details refer to the PDA Diagnostics Window topic in the Millennium32 Online Help Find tab Detector not responding to Millennium 32 Workstation Detector not connected to busLAC E or to LAC E 32 acquisition server in the Millennium 32 Workstation Check IEEE 488 cable connections tighten connectors Incorrect IEEE 488 address 1 Ensure that t...

Page 30: ...sabling user access to Quick Set For details refer to the User Type Properties Dialog Box topic in the Millennium 32 Online Help Find tab Change in reference spectrum Mobile phase contains gas or is contaminated Prepare fresh mobile phase and degas thoroughly Air bubbles trapped in flow cell Flush the flow cell or apply slight backpressure on the detector waste outlet To prevent air bubbles check ...

Page 31: ...ngth readings are accurate Recalibrate the 996 detector only if the Wavelength Accuracy diagnostic in the Internal Diagnostics tests fails You calibrate the 996 detector using the PDA Calibration window which you access from QuickSet and which allows you to View the effects of exposure time on photodiode saturation for a given wavelength range Verify the wavelength location of the deuterium spectr...

Page 32: ... flush with a miscible sovent before switching to methanol 2 If you have been using buffers flush with HPLC quality water at 1 mL min for 10 minutes then switch to methanol for 10 minutes Note Ensure that the solvent is miscible with the previous mobile phase For information on performing calibration refer to the PDA Calibration Window topic in the Millennium 32 Online Help Find tab ...

Page 33: ...stic in the Millennium 32 PDA Diagnostics window fails and the lamp status light is on see Table 2 1 The 996 detector causes high backpressure Note Conditions other than a dirty flow cell may cause decreased lamp intensity For more information refer to Chapter 2 Diagnostics and Calibration Flow cell maintenance consists of Flushing the flow cell Removing the flow cell Cleaning the flow cell Instal...

Page 34: ...components using the procedure described in Section 3 1 2 Removing the Flow Cell 3 1 2 Removing the Flow Cell Note You do not need to shut down the 996 detector to remove and replace the flow cell Required Materials 5 16 inch open end wrench Phillips screwdriver Procedure To remove the 996 detector flow cell 1 Set the flow to 0 0 mL min 2 Power off the solvent delivery system or pump Caution To av...

Page 35: ...low cell assembly to the optics bench and the thumbscrew that secures the bracket holding the fluidic connections then detach the bracket Figure 3 2 Figure 3 2 Removing the Flow Cell Assembly LAMP STATUS TP01461 Flow Cell Access Door Thumbtabs TP01462 Thumbscrews Holding Flow Cell Assembly and Bracket Fluidic Connections Inside Bracket ...

Page 36: ...mage the lenses and the lens holders Attention To prevent contamination use powder free gloves when disassembling inspecting cleaning or replacing parts within the flow cell or when removing or replacing the flow cell within its assembly Attention To prevent contamination use powder free gloves when disassembling inspecting cleaning or replacing parts within the flow cell or when removing or repla...

Page 37: ...ell and lenses 1 Use the TORX T10 screwdriver to remove the three screws that secure one of the lens holder assemblies Figure 3 4 Figure 3 4 Disassembling the Flow Cell 2 Use the small flat blade screwdriver to gently pry the lens assembly from the flow cell body at the slots TP01464 Gasket Flow Cell Disk Lens Assembly Flow Cell Disk Belleville Spring Washer Lens Assembly Flow Cell Body Spring Was...

Page 38: ...ws using the TORX T10 driver to gradually tighten each screw alternating between the screws in a clockwise pattern Tighten until the screws meet the flow cell disk then tighten each screw 1 4 turn If a torque screwdriver is available tighten the screws to 16 in oz 0 113 N m Attention Be careful not to overtighten the screws 6 Repeat steps 1 through 5 to reassemble the other side of the flow cell 3...

Page 39: ...1000 hours of operation You can monitor lamp usage by checking the lamp usage indicator a mercury column with a scale of 0 to 10 where 10 represents 1000 hours As the lamp ages the bubble in the mercury column moves toward the 10 Caution To avoid electrical hazards and exposure to UV light turn off the power and disconnect the power cord before you begin this procedure Note that the lamp and housi...

Page 40: ...40 Maintenance 3 Figure 3 5 Lamp Access Door 4 Examine the lamp usage indicator Figure 3 6 Figure 3 6 Lamp Usage Indicator LAMP STATUS TP01461 Lamp Access Door Thumbtabs TP01466 Lamp Usage Indicator ...

Page 41: ...mination use powder free gloves when removing or replacing the lamp Required Materials TORX T20 screwdriver Caution To avoid electrical hazards when you perform the following procedure power off the 996 detector and disconnect the power cord Procedure To replace the lamp in the 996 detector 1 Power off the 996 detector remove the power cord and allow the lamp to cool for at least 15 minutes Cautio...

Page 42: ...amp by its base orient the lamp so that the notch in the base aligns with the positioning pin in the optics bench 9 Insert the lamp and secure it with the two T20 screws Make sure that the lamp base is flush against the lamp housing 10 Reconnect the lamp power connector see Figure 3 7 11 Close the lamp door and secure it with the thumbtab 12 Install the front panel cover 13 Reconnect the power cor...

Page 43: ...edure Procedure To replace the two fuses in the 996 detector 1 Power off the 996 detector and remove the power cord 2 Locate the fuse block above the power cord plug Figure 3 8 on the rear panel Figure 3 8 Fuse Block 3 Squeeze the two side clips on the fuse block while you pull out the block 4 Remove the fuses from the block then install the new fuses 5 Orient the fuse block with the small tab poi...

Page 44: ...principles of operation of the optics and electronics of the Waters 996 PDA Detector 4 1 996 Detector Optics The 996 detector is an ultraviolet visible light UV Vis spectrophotometer with 512 diodes Optical resolution of 1 2 nm per diode Operating wavelength range from 190 nm to 800 nm The light path through the optics assembly of the 996 detector is shown in Figure 4 1 ...

Page 45: ...erium source lamp through a beamsplitter to the flow cell Beamsplitter and reference diode Reflects part of the light back to a reference diode which measures the intensity of the light emitted by the lamp The detector uses this measurement to keep the lamp output constant Reference Diode Beamsplitter Assembly 50 mm Aperture Lamp and Lamp Optics Spectrographic Mirror and Mask Shutter Assembly Flow...

Page 46: ...hrough the flow cell onto the aperture at the entrance to the spectrographic portion of the optics The mirror mask defines the beam of light focused on the spectrograph mirror Aperture Controls wavelength resolution and intensity of light striking the photodiodes The width of the aperture is 50 µm Shutter assembly Prevents light from reaching the photodiode array except during sampling and calibra...

Page 47: ...fficient to resolve five principal benzene absorption peaks Figure 4 2 Benzene Spectrum at 1 2 nm Resolution 4 3 Measuring Light at the Photodiode The Waters 996 Photodiode Array Detector measures the amount of light striking the photodiode array to determine the absorbance of the sample in the flow cell The array consists of 512 photodiodes arranged in a row Each photodiode acts as a capacitor by...

Page 48: ... time The 996 detector requires 11 msec to sequentially read all of the diodes in the array The minimum exposure time is 11 msec You can set exposure time from 11 to 500 msec For example if an exposure time is set to 50 milliseconds the Waters 996 detector 1 Recharges diode 1 and reads the current required to recharge diode 1 2 Recharges diode 2 and reads the current required to recharge diode 2 3...

Page 49: ...to exposure enabled the 996 detector Calculates exposure time at the start of a run based on maximum light intensity within the wavelength range Limits the exposure so that no diode within the given wavelength range is discharged more than 80 Provides proper settings for signal to noise and dynamic range for each run The Auto Exposure time setting may not support certain sampling rates or waveleng...

Page 50: ... a particular wavelength as specified in the spectra per second sample rate and reports the average as a single data point see Section 4 4 2 Resolution Can apply a filter that acts like an analog filter see Section 4 4 3 Filtering Data 4 4 1 Calculating Absorbance The 996 detector computes absorbance by subtracting the dark current and reference spectrum from the acquired spectrum Absorbance is ba...

Page 51: ...ounts shown in Appendix D Mobile Phase Absorbance Figure 4 4 Absorbance as a Function of Concentration Dark Current Photodiodes lose charge over time even when they are not exposed to light The amount of charge lost is called dark current At the start of a chromatographic run the 996 detector closes the shutter to take a dark current reading for each diode The shutter closes after the exposure tim...

Page 52: ...g the following equation where S Signal obtained during sample analysis D Signal obtained during the dark test R Signal obtained from the reference spectrum n Diode number 4 4 2 Resolution The data reported by the 996 detector to the Millennium 32 database can be the average of a number of data points After calculating absorbance the detector averages absorbance values based on Spectral resolution...

Page 53: ...range spectral resolution and sample rate which are set in the General tab of the 996 PDA Instrument Method Editor For details refer to the Waters 996 PDA Detector Properties topic in the Millennium32 Online Help Find tab 4 4 3 Filtering Data Use the Channel 1 tab of the 996 PDA Instrument Method Editor for details refer to the Waters 996 Detector Properties topic in the Millennium32 Online Help F...

Page 54: ...iple compounds in the same peaks coelution or due to nonideal conditions such as noise photometric error or solvent effects 5 1 Comparing Absorbance Spectra The shape of an absorbance spectrum is determined by the relative absorbance at different wavelengths The shape of a compound s absorbance spectrum is a characteristic of that compound at the solvent and pH conditions under which the absorbanc...

Page 55: ...bsorbance spectrum Direction is independent of concentration Vector direction contributes to the identification of a compound since the direction is a function of the absorbance spectrum of the compound The ability of spectral vectors to differentiate compounds depends on the resolution of spectral features As both wavelength range and spectral resolution increase the precision of a spectral vecto...

Page 56: ...rent from that of the vector for Compound A The difference in direction which reflects the difference in the absorbance ratios of the two compounds at wavelengths 245 nm and 257 nm is called the Spectral Contrast Angle A Spectral Contrast angle e g θ in Figure 5 2 greater than zero indicates a shape difference between spectra see Section 5 3 Spectral Contrast Angles The length of the vector is pro...

Page 57: ... two vectors of any two spectra the Spectral Contrast angle quantifies the magnitude of the shape difference between the spectra The Spectral Contrast angle is the difference in direction between the spectral vectors of two spectra A Spectral Contrast angle can vary from 0 degrees to 90 degrees A Spectral Contrast angle near 0 degrees indicates little shape difference between the compared spectra ...

Page 58: ...ectral Contrast Angle Spectra with Similar Shapes In Figure 5 4 the absorbance spectra of two compounds A and B are similar and therefore have a small Spectral Contrast angle 3 0 Normalized Absorbance Wavelength nm Compound A Compound B Spectral Contrast Angle 62 3 ...

Page 59: ...d may exhibit slight differences because of detector noise photometric error high sample concentration or variations in solvent conditions The spectra in Figure 5 5 for example show how instrument noise can affect the shape of an absorbance spectrum of one compound This effect is most likely to occur at low concentrations where the signal to noise ratio is low Note that the Spectral Contrast angle...

Page 60: ...geneity You can assess the significance of spectral inhomogeneity by comparing a Spectral Contrast angle to a Threshold angle see Section 5 4 4 5 4 1 Detector Noise Statistical and thermal variations add electrical noise to the absorbance measurements made by the 996 detector The noise manifests itself as fluctuations in the baseline known as baseline noise The magnitude of any absorbance differen...

Page 61: ...lvent Changes As long as solvent concentration and composition do not change isocratic operation the background absorbance if any by the solvent remains constant A change however in solvent pH or composition such as occurs in gradient operation can affect the intrinsic spectral shape of a compound as shown in Figure 5 6 5 4 4 Threshold Angle In addition to computing Spectral Contrast angles the Sp...

Page 62: ...0 300 320 340 pH 6 9 pH 5 1 pH 3 1 NH 3 NH 2 NH 2 COOH COOH Effect of pH p Aminobenzoic Acid COOH 200 220 240 260 280 300 320 340 Effect of Acetonitrile Concentration p Aminobenzoic Acid COOH NH 2 2 10 Acetonitrile Wavelength Absorbance Absorbance nM Effect of pH p Aminobenzoic Acid pH 3 1 pH 5 1 pH 6 9 COO Effect of Acetonitrile Concentration p Aminobenzoic Acid ...

Page 63: ...cm Weight 31 lbs 14 3 kg Wavelength range 190 to 800 nm Wavelength accuracy 1 nm Linearity range 1 1 Per ASTM 685 79 5 at 2 0 AU propylparaben at 258 nm Spectral resolution 1 2 nm Baseline noise 1 5 10 5 AU peak to peak dry at 254 nm Drift 1 10 3 AU hour at 254 nm after warmup Flow cells Standard Semi preparative Microbore Inert Pathlength mm Tubing I D 10 0 009 in 3 0 040 in 3 0 005 in 10 0 010 i...

Page 64: ...Flow cell standard WAT057919 Flow cell semi preparative WAT057463 Flow cell microbore WAT057462 Flow cell inert WAT057461 Gasket flow cell 2 WAT057924 Belleville washer 2 WAT057925 Lens mount and lens 2 WAT057923 Semi prep lens kit WAT057968 Deuterium lamp WAT057760 Fuse 4 A 5 20 mm WAT057337 Waters Erbium Perchlorate Wavelength Accuracy Solution WAT042885 Waters Absorbance Detector Linearity Solu...

Page 65: ...edures and that during the Warranty period the performance of all components of the Waters 996 PDA Detector other than Third Party Components non Waters named will not deviate materially from the Specifications for such detectors Warranties if any that may be applicable to Third Party Components shall be provided by the respective manufacturers or suppliers of such Third Party Components and Water...

Page 66: ...the purchased Waters 996 PDA Detector or refund of all amounts theretofore paid by Customer to Waters Corporation for the Waters 996 PDA Detector Disclaimers THE LIMITED WARRANTY SET FORTH HEREIN IS EXCLUSIVE AND IN LIEU OF AND CUSTOMER HEREBY WAIVES ALL OTHER REPRESENTATIONS WARRANTIES AND GUARANTEES EXPRESS OR IMPLIED INCLUDING WITHOUT LIMITATION ANY IMPLIED WARRANTIES OF MERCHANTABILITY FITNESS...

Page 67: ...ountry of the selling company Warranty service is provided during business hours 8 AM to 5 PM EST Monday through Friday Service is not available when Waters offices are closed in observance of legal holidays Warranty Service Exceptions Warranty service is not performed on Any product or part that has been repaired by others improperly installed altered or damaged in any way Products or parts ident...

Page 68: ...rranty period the replacement part is warranted for the balance of the original warranty period Table C 1 summarizes the warranty periods for applicable components Table C 1 Warranty Periods Lamp Replacement Warranty The Waters 996 PDA Detector deuterium lamp is warranted to light and pass powerup verification tests for 1000 hours Item Warranty Waters 996 PDA Detector 1 year Deuterium lamp 1000 ho...

Page 69: ...discovered cease further unpacking of the unit involved and request immediate inspection by local agent or carrier and secure written report of his findings to support claim This request must be made within 15 days of receipt Otherwise the claim will not be honored by the carrier Do not return damaged goods to the factory without first securing an inspection report and contacting Waters for a retu...

Page 70: ...ance that is autozeroed out Wavelength pH and concentration of the mobile phase will affect its absorbance Examples of several mobile phases are provided below Table D 1 Mobile Phase Absorbance Measured Against Air or Water Absorbance at Specified Wavelength nm 200 205 210 215 220 230 240 250 260 280 Solvents Acetonitrile 0 05 0 03 0 02 0 01 0 01 0 01 Methanol not degassed 2 06 1 00 0 53 0 37 0 24...

Page 71: ...2 50 2 45 2 37 1 96 0 50 0 12 0 04 0 01 Buffers and Salts Ammonium acetate 10 mM 1 88 0 94 0 53 0 29 0 15 0 02 0 01 Ammonium bicarbonate 10 mM 0 41 0 10 0 01 0 01 EDTA disodium 1 mM 0 11 0 07 0 06 0 04 0 03 0 03 0 02 0 02 0 02 0 02 HEPES 10 mM pH 7 6 2 45 2 50 2 37 2 08 1 50 0 29 0 03 0 01 MES 10 mM pH 6 0 2 42 2 38 1 89 0 90 0 45 0 06 0 01 Table D 1 Mobile Phase Absorbance Measured Against Air or...

Page 72: ...te 10 mM 2 48 2 84 2 31 2 02 1 49 0 54 0 12 0 03 0 02 0 01 Sodium formate 10 mM 1 00 0 73 0 53 0 33 0 20 0 03 0 01 Sodium phosphate 100 mM pH 6 8 1 99 0 75 0 19 0 06 0 02 0 01 0 01 0 01 0 01 0 01 Tris HCl 20 mM pH 7 0 1 40 0 77 0 28 0 10 0 04 0 01 Tris HCl 20 mM pH 8 0 1 80 1 90 1 11 0 43 0 13 0 01 Table D 1 Mobile Phase Absorbance Measured Against Air or Water Continued Absorbance at Specified Wa...

Page 73: ... 02 0 02 0 02 0 01 Detergents BRIJ 35 1 0 06 0 03 0 02 0 02 0 02 0 01 0 01 CHAPS 0 1 2 40 2 32 1 48 0 80 0 40 0 08 0 04 0 02 0 02 0 01 SDS 0 1 0 02 0 01 0 01 Triton X 100 0 1 2 48 2 50 2 43 2 42 2 37 2 37 0 50 0 25 0 67 1 42 Tween 20 0 1 0 21 0 14 0 11 0 10 0 09 0 06 0 05 0 04 0 04 0 03 Table D 1 Mobile Phase Absorbance Measured Against Air or Water Continued Absorbance at Specified Wavelength nm ...

Page 74: ...fluidic 23 non IEEE 488 19 rear panel 16 terminal strip 22 Contact closures 19 Contacting Waters Technical Service 27 31 D Damage warranty 69 Dark current 51 Data acquisition Auto Exposure parameter 49 Exposure Time parameter 49 Derived vectors 56 56 Diagnostics 30 DIP switch setting 18 E Electrical connections 15 Events connections 19 22 22 electrical specifications 21 terminal strip connections ...

Page 75: ...nce flow cell 33 fuse 43 lamp 39 PDA detector 33 43 Match Angle photometric error effects 61 Maximum absorbance 61 Millennium Chromatography Manager connections 16 Mobile phase absorbances 70 wavelengths 70 N Network address 18 Noise effects 60 Nonidealities 60 Non IEEE 488 connections 19 O Outputs 19 21 22 22 P Parts spare 64 Photodiode array 47 Photometric error 61 61 Power connections 15 Purity...

Page 76: ...ld angle 60 Troubleshooting 27 32 Tubing cutting 23 V Vectors derived from multiple wavelengths 56 derived from two wavelengths 56 spectra representing 55 spectral contrast 55 W Warranty claims 69 damages 69 disclaimers 66 exclusions 65 information 65 68 lamp replacement 68 limited 65 period 68 returns 69 service 67 service exceptions 67 Waters 996 absorbance calculations 50 52 aperture width 47 d...

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