Plumbing the instrument
2-9
Top view of tubing guide
Tip:
For Waters connections, the startup kit includes stainless steel and
PEEK plastic fittings.
To connect the tubing
1.
Connect the pump inlet line (blue) to the pump.
Tip:
Route the tubing through one of the square cut-outs on either side of
the instrument.
2.
Place the red line in a waste container.
3.
Set the mobile phase pump to deliver 1 mL/min of 100% methanol or
acetonitrile for 10 minutes.
Recommendation:
Before connecting the column, flush the system
according to the following procedure.
4.
Route the red line through one of the square cut-outs on either side of
the instrument, and then connect it to the column.
Tip:
When a Waters 1500 series pump and column heater are used, both
the red and blue lines are likely to exit the instrument through the
left-hand cut-out.
5.
Set pump flow, and check for leaks.
Recommendation:
Allow the system to stabilize for 5 minutes.
