Waters 2475, Operator'S Manual, Page: 101 / 228

Operating the detector 3-29
Two operating modes
You can use the detector in either single-channel or multichannel mode over a
range of 200 to 900 nm. The detector defaults to its mode of operation when
last shut down.
When the detector is operating in single-channel mode, you can configure
analog outputs on channel B. In single-channel mode, the detector tracks a
single wavelength on both channels A and B. You can use channel B for these
purposes:
• Track fluorescence (EU) at an alternate EUFS.
• Monitor sample or reference energy outputs.
• Set a different time-constant.
The table titled “Primary and secondary function (method) parameters” on
page 3-21 includes more information on operating parameters adapted in
single or multichannel mode. When an Empower or Millennium
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data system
controls the detector, it functions as a Waters 474 detector (see page 3-29 ).
Standalone operation
When using the detector as a standalone instrument, you can store up to 10
methods, each containing up to 48 timed and 2 threshold events (see
page 3-43 ). An asterisk in the method number field on the home screen
indicates conditions at the time of a run, not a stored method.
Remote control operation for 474 emulation mode via RS-232
The remote control icon appears on the home screen (see the figure “Finding
parameters in the fluorescence home screen” on page 3-4 and the table titled
“2475 detector home and message screen icons” on page 3-5 ) when an external
data system controls the detector. Under Empower or Millennium
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control for
474 emulation, the detector uses the RS-232 connector (see page 2-18 ), and an
“R” appears within the remote icon, which itself appears on the busLAC/E
data system’s configuration screen as a 474 detector when the Emulate 474
option is enabled and operating in single-channel mode.
To connect the detector to an HPLC system, see
page 2-3 . To connect the
detector to an external system, see page 2-5 .