PC-505B Manual, Rev. 201013
Warner
Instruments
A Harvard Apparatus Company
7
outside-out patch and whole cell preparations, this corresponds to the conventional
physiological definition of outward transmembrane current. With inside-out or cell-attached
patches, the physiological transmembrane current equals minus the indicated I
m
.
Membrane potential (Vm)
Membrane potential is defined as pipet potential minus bath potential. With outside-
out patch and whole cell preparations, this corresponds to the conventional physiological
definition of transmembrane potential. With inside-out or cell-attached patches, the
physiological transmembrane potential equals minus the indicated V
m
.
