PC-505B Manual, Rev. 201013
Warner
Instruments
A Harvard Apparatus Company
24
10.
Set
GAIN
to
10 mV/pA.
11.
Verify that oscilloscope reads +1 V (1 div at these settings)
12.
Set
VOLTAGE HOLD
to
negative
(
–
).
13.
Verify that
METER
reads
–
10 pA
.
14.
Verify that oscilloscope reads
–
1 V (1 div at these settings)
15.
Return amplifier to initial settings
Cap Comp checkout
1.
Connect model cell to headstage in
patch mode
.
2.
Set
PROBE RESISTOR
to
high
3.
Set
VOLTAGE HOLD
control to
off
.
4.
Set scope to 5 V/div.
5.
Set
COMMAND SELECT
to
internal command
.
6.
Set
COMMAND SENSITIVITY
toggle to
on.
7.
Scope should now display large amplitude positive and negative pulses. Clipping LED
will be lit.
8.
Using
FAST CAP COMP
controls, minimize amplitude and duration of pulses. (Leading edge
transients will remain due to characteristics of model cell.)
9.
Set
PROBE RESISTER
to
low
.
10.
Connect model cell to headstage in
whole
cell mode
.
11.
Turn
C
–
SLOW
toggle switch
on.
12.
Adjust
C
–
SLOW
and
SERIES R
controls until leading edge of square wave transitions
smoothly from one peak to the other. Full transition should take approximately 1 ms.
Remaining leading edge transients can be minimized by small adjustments to the
FAST
CAP COMP
controls.
13.
Turn %
CORRECTION
toggle switch
on
. Verify that control decreases transition time
without increasing amplitude of spike.
14.
Turn %
CORRECTION
off
.
15.
Turn
LEAK SUBTRACTION
on
.
16.
Verify that control adjusts amplitude of square wave.
17.
Remove model cell from headstage.
18.
Return amplifier to initial settings.
Current clamp checkout
1.
Attach model cell to headstage in
bath mode
.
2.
Set
MODE
toggle to
I
o.
3.
Set
METER SELECT
to
V
m
.
4.
Using
JUNCTION ZERO
control adjust
METER
to
0 mV.
