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Warner Instruments BC-535, Manual, Page: 10 / 41

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Warner Instruments BC-535 Manual Download Page 10

BC-535 Preliminary, Rev. 060126 

 

10

 

 

Note: We recommend separating the circuit and chassis grounds by disconnecting the bridging bar 
between the associated ground posts. Loosen the posts and slide the bridge to one side. 

Gain Telegraph  

The 

GAIN TELEGRAPH

 is a stepped voltage output designed to communicate the instrument gain 

setting to your acquisition software. DC voltages are stepped from 0.0 V to 5.5 V, in steps of 500 mV. 

GAIN TELEGRAPH

 voltage outputs for the associated amplifier  I

m

 

GAIN

 are specified below. (I

m

 

GAIN

 

settings are selectable in the front panel 

OUTPUTS

 block, see page 8) 

 

I

m

 Gain (mV/pA) 

Gain Telegraph (V) 

standby 0.0 

0.5 0.5 

1 1.0 

2 1.5 

5 2.0 

10 2.5 

20 3.0 

50 3.5 

100 4.0 

200 4.5 

500 5.0 

1000 5.5 

 

Filter Telegraph 

The 

FILTER TELEGRAPH

 is a stepped voltage output designed to communicate the instrument filter 

cutoff frequency setting to your acquisition software. DC voltages are stepped from 0.5 V to 5.0 V, in 
steps of 500 mV and are specified below. (

FILTER

 settings are selectable in the front panel 

OUTPUTS

 

block, see page 8) 

 

Filter Frequency (Hz) 

Filter Telegraph (V) 

50 0.5 

100 1.0 

200 1.5 

500 2.0 

1k 2.5 

2k 3.0 

5k 3.5 

10k 4.0 

20k 4.5 

bypass 5.0 

 

 

            Warner

 Instruments 

A Harvard Apparatus Company   . 

Summary of Contents for BC-535

Page 1: ...ne er r I In ns st tr ru um me en nt ts s B Bi il la ay ye er r C Cl la am mp p A Am mp pl li if fi ie er r M Mo od de el l B BC C 5 53 35 5 Warner Instruments 1125 Dixwell Avenue Hamden CT 06514 800...

Page 2: ...r panel 9 Headstage 9 Circuit and chassis grounds 9 Gain Telegraph 10 Filter Telegraph 10 Im output 11 External Command In 11 Capacitance Output 11 Cap Sync Out 11 External speaker 11 ADDITIONAL INFOR...

Page 3: ...ozero 21 Capacity compensation 22 OPERATION 23 Setup of the bilayer chamber 23 Input offset 24 Input offset adjustment 24 Bilayer formation 24 Commands 25 APPENDIX 26 Theoretical considerations 26 Shi...

Page 4: ...nge of the digital hold control has been extended to 400 mV for internally generated commands and the amplifier supports up to 1 V at the external command input for a sum capability of 1400 mV hold po...

Page 5: ...MALL CAPS References to specific controls within a block are specified using NON UNDERLINED SMALL CAPS Finally references to individual control settings are specified in italic type Special comments a...

Page 6: ...ential The maximum range for this control is 400 mV The internally generated HOLD command can be disabled by selecting the off position on the ON OFF TOGGLE SWITCH to the right of the meter Note The M...

Page 7: ...tment is only available when the offset circuit is armed Fine adjustment of the rotary control can be achieved by pressing the control in while turning Low high LED s are provided to indicate which di...

Page 8: ...FILTER TELEGRAPH BNC on the instrument rear panel The AUDIO section is comprised of an on off toggle and volume control Audio output is useful during membrane formation to monitor the successful appl...

Page 9: ...ops Circuit and chassis grounds CIRCUIT and CHASSIS GROUND binding posts are provided at the rear of the amplifier to allow modification of instrument grounding The CHASSIS GROUND binding post is inte...

Page 10: ...d below Im GAIN settings are selectable in the front panel OUTPUTS block see page 8 Im Gain mV pA Gain Telegraph V standby 0 0 0 5 0 5 1 1 0 2 1 5 5 2 0 10 2 5 20 3 0 50 3 5 100 4 0 200 4 5 500 5 0 10...

Page 11: ...f the resulting square wave The SYNC OUT signal is a standard TTL square wave and is 100 s in duration External speaker A standard RCA jack is provided for attachment to an external speaker for use in...

Page 12: ...mplifier The MC 1 contains a 100 pF capacitor connected in parallel with a 1 G resistor The precision of this resistor is 5 The MC 1 connects to the two 1 mm mini jacks on the headstage marked INPUT a...

Page 13: ...a basic BLM layout is shown in Figure 1 Warner Instruments provides all components used in the assembly of a BLM workstation including Faraday cages vibration isolation tables a dedicated bilayer clam...

Page 14: ...cal noise is critical to increasing the signal to noise ratio of a BLM workstation The significance of this becomes apparent when one considers that the acoustic coupling of normal speech to the buffe...

Page 15: ...gn employed by Warner cups Another aperture geometry commonly used is that of a conical hole with the small end of the hole supporting the bilayer membrane This geometry often employed on custom made...

Page 16: ...ata has been acquired and stored it must be analyzed for its biophysical characteristics Since the volume of data collected is often exceedingly large analysis is usually performed by dedicated softwa...

Page 17: ...membrane Several techniques for solution exchange are available These include gravity feed pump driven devices or manually applied pressure driven systems In general fresh solution is added to the bo...

Page 18: ...plifier and can also be used re assess the performance of the amplifier at a later time To perform these tests you will need BC 535 Bilayer Clamp amplifier included headstage included model membrane F...

Page 19: ...auto trigger DC coupling on the input channel Turn on both the BC 535 and the oscilloscope Hold voltage test Connect the model membrane MC 1 to the headstage inputs and the green wire from the model...

Page 20: ...UP DOWN TOGGLE switches in the HOLD block adjust hold potential until the meter reads 0 mV Place the amplifier into standby Input noise test without model membrane Remove the model membrane Insure tha...

Page 21: ...st With the model membrane still connected set the oscilloscope voltage base to 50 mV div Set the Im GAIN on the amplifier to 1 mV pA Place the amplifier into operate Activate CAP TEST mode by setting...

Page 22: ...observe the resulting signal on the oscilloscope You will see a large transient on the leading edge of both the upward and downward excursions of the signal This transient or spike represent the larg...

Page 23: ...ngenuity and training once the hole has been coated the cup is inserted into the chamber and both the cup and chamber filled with the appropriate solutions Two methods are presented below One method t...

Page 24: ...CTOR SWITCH is in the offset position If using the manual approach the control should be advanced slowly since a small change in rotation will result in a large change in the current through the open...

Page 25: ...a thin lipid film across the open aperture reminiscent of making bubbles when you were a kid Alternatively the membrane can be formed by momentarily occluding the hole with the end of the lipid coate...

Page 26: ...then connected via a 14 16 gauge braided copper wire to an external central ground point which acts as the absolute reference for all devices A bar of this type is provided in Faraday cages supplied b...

Page 27: ...ground Doing so will create a ground loop and increase noise levels within the data This condition can be avoided by disconnecting the oscilloscope chassis ground from the common ground point when con...

Page 28: ...h in meters Likewise we know that that the steady state charge on a capacitor can be expressed as CV q 2 where q is the charge on one capacitor plate in Coulombs and V is the potential between the pla...

Page 29: ...nstrument gain For example a 100 pF membrane would yield a 1000 mV square wave p p when the amplifier gain is set to 10 mV pA Suggested References 1 Ion Channel Reconstitution edited by C Miller Plenu...

Page 30: ...l selectable from 0 5 to 200 kHz in 1 2 5 steps or bypassed for full amplifier bandwidth Capacity compensation FAST 0 10 s and SLOW 0 10 ms with adjustment of amplitude and time constant for each rang...

Page 31: ...1999 pA full scale Power 100 125 or 220 240 VAC 50 60 Hz Dimensions H W D Case 9 42 25 cm 3 5 16 5 10 in Headstage 2 3 2 8 5 8 cm 0 9 1 1 2 25 in 1 8 m connecting cable Operating Conditions Equipment...

Page 32: ...unused wire should be cleaned with ETOH before chloriding Previously chlorided wire should be cleaned before re chloriding Two methods for chloriding most commonly used are the plating techniques des...

Page 33: ...als and workmanship for a period of three years from the date of shipment If a failure occurs within this period we will either repair or replace the faulty component s This warranty does not cover in...

Page 34: ...panel will loosen after long use These are collet style knobs and are tightened with a screw located under the knob cap To gain access to the adjustment screw pry the cap off with a thin bladed screwd...

Page 35: ...000 4 8 EN610000 4 11 Manufacturer s Name Warner Instruments Manufacturer s Address 1125 Dixwell Avenue Hamden CT 06514 Tel 203 776 0664 Equipment Description Instrument Amplifier Equipment Class ITE...

Page 36: ...ments Manufacturer s Address 1125 Dixwell Avenue Hamden CT 06514 Tel 203 776 0664 Equipment Description Instrument Amplifier Safety requirements for electrical equipment for measurement and laboratory...

Page 37: ...BC 535 Preliminary Rev 060126 37 BC 535 Preliminary Rev 060126 37 Warner Instruments A Harvard Apparatus Company Warner Instruments A Harvard Apparatus Company...

Page 38: ...ar or become black Alternatively Bilayer Lipid Membrane Many researchers now observe membrane formation electrically and have altered the acronym to mean the molecular bilayer formed from the orientat...

Page 39: ...ransmembrane potential It should be noted that by this definition a transmembrane potential of 100 mV is still depolarized digital Quantized or discrete Normally refers to information manipulated by a...

Page 40: ...in the closed state mean open time The average length of time a gating channel will remain in the open state mini jack A small plug on the headstage to which the electrodes are attached model membrane...

Page 41: ...le variable resistor used for making fine adjustments to a circuit TTL Transistor Transistor Logic Voltage ranges used to define an on or off state in binary devices 0 0 8 V defines a logic 0 state an...

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