2
IMPORTANT
This is a quick guide for the use of MD Stainer and procedure for the fluorescence in situ hybridization
technique (FISH), which include the most relevant information for the use, maintenance and cleaning of
the instrument, as well as instructions for the preparation of tissue sections. For more detailed
information, you can consult the
User Manual
.
For the automated use of the equipment, the user must have been given a previous training by the
equipment supplier.
HYBRIDIZATION AREA
In order to guarantee the quality and reliability of the hybridization, the sections must be prepared
according to the following guidelines:
-
In the picture below,
the obligatory position for the tissue
is marked with a red rectangle.
-
The area highlighted in red shows the
hybridization area
. That is why placing the section in the
indicated area is important.
PREPARATION AND PRETREATMENT OF PARAFFIN-EMBEDDED SECTIONS
Section
Paraffin-embedded tissue sections are recommended to be
2-3 µm
thick maximum and to place
them on the slide respecting the
tissue placement area recommended
in the previous section.
Note: in order to obtain optimal results, it is recommended to prepare the sections a day in advance
maximum. If they are made earlier, it is recommended to heat them for a maximum of 5 minutes so that
they adhere to the slide correctly and to leave them at room temperature in a protected area until they are
to be stained (maximum one week).