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7

4. Functional testing

Mount new pipette tip and

set the nominal volume

Take up the test liquid

  Aspiration of the liquid not possible or very slow:

If a drop forms: 

 

The pipetting button must move smoothly and 

See notes in the table below

Leak test: Hold the filled pipette
vertically for approx. 10 seconds and 
observe if a drop forms at the tip

See notes in the table below

Release the test liquid. Hold pipette tip
against wall of the vessel and wipe off
against the wall

jerk-free

Eject the tip

Enter results in the test record

Possible errors and the resulting measures

(For other measures and more troubleshooting advice, please check the operating manual)

Error

Possible cause

Measures

Aspiration not possible

or very slow

Pipette shaft or pipette shaft
tip blocked

Clean the instrument

(see operating manual)

Drop forming at the

pipette tip

Pipette tip not properly attached

Seal or piston damaged

Attach new pipette tip

Clean or replace seal and / or

piston
(see operating manual)

Replace V- resp. O-ring

(see operating manual)

V- resp. O-ring damaged

Summary of Contents for micropipette

Page 1: ...Standard Operating Procedure SOP VITLAB micropipette VITLAB GmbH Linus Pauling Str 1 63762 Grossostheim Germany tel 49 6026 97799 0 fax 49 6026 97799 30 info vitlab com www vitlab com...

Page 2: ...Table of contents 1 Introduction 3 2 Preparation of the pipette for testing 4 3 Test instruments and environment 6 4 Functional testing 7 5 Calibration Checking the volume 8 6 Evaluation 9 7 Tables 1...

Page 3: ...01 DIN EN ISO 10012 and DIN EN ISO IEC 17025 Owing to its 8 respectively 12 channels the VITLAB micropipette 8 12 can carry out 8 resp 12 pipettings in one action However this advantage results in an...

Page 4: ...ready VITLAB micropipette Use only manufacturerer s original parts Pipette tips Use only appropriate tips Optimum results can only be obtained with VITLAB pipette tips 2 3 Cleaning Clean pipette shaf...

Page 5: ...ing manual Error Possible cause Measures Pipette tip is no longer tight Scratches on the pipette shaft tip Obtain spare parts see operating manual Device is stiff and or leaking Seal Piston soiled or...

Page 6: ...ent error of 0 2 C Hygrometer Considering the measuring tolerance of the hygrometer a relative atmospheric humidity of 40 60 should be reached Balance Recommended specifications see table Selected vol...

Page 7: ...wall of the vessel and wipe off against the wall jerk free Eject the tip Enter results in the test record Possible errors and the resulting measures For other measures and more troubleshooting advice...

Page 8: ...standards to the national standard under defined conditions 5 Calibration Checking the volume 1 Set the nominal volume at 10 or 20 Enter temperature into test record 2 Determine temperature of the tes...

Page 9: ...8 values for the 12 channel pipette 6 Evaluation The obtained weighing values from the gravimetric test are only the mass values of the pipetted volume without the correction of the air buoyancy To ob...

Page 10: ...n the operating manual Recalibrate the VITLAB micropipette according to the steps in the operating manual If these measures are not successful we recommend you to make use of the VITLAB calibration se...

Page 11: ...tor z ml g Temperature C 1 0020 1 0020 23 23 5 1 0035 1 0036 16 16 5 1 0021 1 0022 24 24 5 1 0038 1 0039 17 17 5 1 0023 1 0024 25 25 5 1 0040 1 0041 18 18 5 1 0025 1 0026 26 26 5 1 0043 1 0044 19 19 5...

Page 12: ...6 10 5 1 20 10 2 100 50 10 200 100 20 1000 500 100 5000 2500 500 10000 5000 1000 1 2 0 8 1 0 0 5 0 8 0 3 0 8 0 3 0 8 0 3 0 8 0 3 0 6 0 3 For calibration the error limits to be observed by the operator...

Page 13: ...1 2 3 0 0 3 0 6 1 5 10 5 1 50 25 5 100 50 10 200 100 20 300 150 30 2 4 1 6 2 0 0 8 1 6 0 6 1 6 0 6 1 6 0 6 For calibration the error limits to be observed by the operator must individually defined by...

Page 14: ...ominal volume Serial number Customers identification 5 Weighing values and results of the gravimetric test VITLAB continuous E RS VITLAB genius VITLAB simplex VITLAB TA VITLAB micropipette Weighing va...

Page 15: ...st 35 Correction factor Z 1 Instrument Type VITLAB micropipette 8 12 8 channel 12 channel Nominal volume Serial number Customers identification Channel No Weighing value 1 2 3 4 5 6 7 8 9 10 11 12 X1...

Page 16: ...inal volume mg A nominal CV nominal Channel No Weighing values 1 2 3 4 5 6 7 8 9 10 11 12 X1 mg X2 mg X3 mg X4 mg X5 mg X6 mg X7 mg X8 mg X9 mg X10 mg X mean mg V mean l A found CV found A nominal CV...

Page 17: ...nal Channel No Weighing values 1 2 3 4 5 6 7 8 9 10 11 12 X1 mg X2 mg X3 mg X4 mg X5 mg X6 mg X7 mg X8 mg X9 mg X10 mg X mean mg V mean l A found CV found A nominal CV nominal Result A Result CV The t...

Page 18: ...e been carefully cleaned and decontaminated before shipment That the instruments pose no danger through bacteriological chemical radiological or viral contamination That she he is aware that shipment...

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