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7.2 Volume error limits for piston operated pipettes

Table 7.2.1: Excerpt from DIN EN ISO

Nominal volume

µl

A

 ± %

CV*

 %

10

20

100

200

1000

5000

10000

Nominal volume

µl

A

 ± %

CV*

 %

8655, Part 2

Table 7.2.2: Values from operating manual

VITLAB   micropipette

®

The stated volume error limits for the VITLAB   micropipette (Table 7.2.2) are final test values relative
to the nominal capacity. These error limits refer to new instruments under optimised test conditions

(qualified operators and standardised ambience conditions). Typically these error limits are two times

®

better under ideal testing conditions (experience of the manufacturer). For partial volumes the absolute
value (µl) corresponding to the nominal volume is applied.

 

1

 

1.6

 

7

0,5
1
4

 

0.8

 

1.2

 

5

0.4
0.7
2

0.6
0.8
3

0.2
0.4
1

0.6
0.8
3

0.2
0.3
0.6

0.6
0.8
3

0.2
0.3
0.6

0.6
0.8
3

0.2
0.3
0.6

0.6
0.8
3

0.2
0.3
0.6

10

5
1

20
10

2

100

50
10

200
100

20

1000

500
100

5000
2500

500

10000

5000
1000

1.2

0.8

1.0

0.5

0.8

0.3

0.8

0.3

0.8

0.3

0.8

0.3

0.6

0.3

For calibration, the error limits to be observed by the operator must individually defined by the user.
For this purpose, the following methods can be applied:

If required by the application and if the optimised conditions for measuring are present, the stated

In correspondence to the German regulations for official testing, it is also admissible to apply limits

The user may also define his own individual tolerance limits corresponding to his particular applicat-

error limits can also be expected in the case of used volumetric instruments are in good working
order.

which are typical for practice. These practice limits correspond to double the limits for official testing.

In this case, the values in table 7.2.2. should be doubled!

ion, and apply these error limits for the calibration of the instrument.

The above procedures fulfil the requirements of DIN EN ISO 9001, DIN EN ISO 10012 and DIN EN 
ISO / IEC 17025.

Summary of Contents for micropipette

Page 1: ...Standard Operating Procedure SOP VITLAB micropipette VITLAB GmbH Linus Pauling Str 1 63762 Grossostheim Germany tel 49 6026 97799 0 fax 49 6026 97799 30 info vitlab com www vitlab com...

Page 2: ...Table of contents 1 Introduction 3 2 Preparation of the pipette for testing 4 3 Test instruments and environment 6 4 Functional testing 7 5 Calibration Checking the volume 8 6 Evaluation 9 7 Tables 1...

Page 3: ...01 DIN EN ISO 10012 and DIN EN ISO IEC 17025 Owing to its 8 respectively 12 channels the VITLAB micropipette 8 12 can carry out 8 resp 12 pipettings in one action However this advantage results in an...

Page 4: ...ready VITLAB micropipette Use only manufacturerer s original parts Pipette tips Use only appropriate tips Optimum results can only be obtained with VITLAB pipette tips 2 3 Cleaning Clean pipette shaf...

Page 5: ...ing manual Error Possible cause Measures Pipette tip is no longer tight Scratches on the pipette shaft tip Obtain spare parts see operating manual Device is stiff and or leaking Seal Piston soiled or...

Page 6: ...ent error of 0 2 C Hygrometer Considering the measuring tolerance of the hygrometer a relative atmospheric humidity of 40 60 should be reached Balance Recommended specifications see table Selected vol...

Page 7: ...wall of the vessel and wipe off against the wall jerk free Eject the tip Enter results in the test record Possible errors and the resulting measures For other measures and more troubleshooting advice...

Page 8: ...standards to the national standard under defined conditions 5 Calibration Checking the volume 1 Set the nominal volume at 10 or 20 Enter temperature into test record 2 Determine temperature of the tes...

Page 9: ...8 values for the 12 channel pipette 6 Evaluation The obtained weighing values from the gravimetric test are only the mass values of the pipetted volume without the correction of the air buoyancy To ob...

Page 10: ...n the operating manual Recalibrate the VITLAB micropipette according to the steps in the operating manual If these measures are not successful we recommend you to make use of the VITLAB calibration se...

Page 11: ...tor z ml g Temperature C 1 0020 1 0020 23 23 5 1 0035 1 0036 16 16 5 1 0021 1 0022 24 24 5 1 0038 1 0039 17 17 5 1 0023 1 0024 25 25 5 1 0040 1 0041 18 18 5 1 0025 1 0026 26 26 5 1 0043 1 0044 19 19 5...

Page 12: ...6 10 5 1 20 10 2 100 50 10 200 100 20 1000 500 100 5000 2500 500 10000 5000 1000 1 2 0 8 1 0 0 5 0 8 0 3 0 8 0 3 0 8 0 3 0 8 0 3 0 6 0 3 For calibration the error limits to be observed by the operator...

Page 13: ...1 2 3 0 0 3 0 6 1 5 10 5 1 50 25 5 100 50 10 200 100 20 300 150 30 2 4 1 6 2 0 0 8 1 6 0 6 1 6 0 6 1 6 0 6 For calibration the error limits to be observed by the operator must individually defined by...

Page 14: ...ominal volume Serial number Customers identification 5 Weighing values and results of the gravimetric test VITLAB continuous E RS VITLAB genius VITLAB simplex VITLAB TA VITLAB micropipette Weighing va...

Page 15: ...st 35 Correction factor Z 1 Instrument Type VITLAB micropipette 8 12 8 channel 12 channel Nominal volume Serial number Customers identification Channel No Weighing value 1 2 3 4 5 6 7 8 9 10 11 12 X1...

Page 16: ...inal volume mg A nominal CV nominal Channel No Weighing values 1 2 3 4 5 6 7 8 9 10 11 12 X1 mg X2 mg X3 mg X4 mg X5 mg X6 mg X7 mg X8 mg X9 mg X10 mg X mean mg V mean l A found CV found A nominal CV...

Page 17: ...nal Channel No Weighing values 1 2 3 4 5 6 7 8 9 10 11 12 X1 mg X2 mg X3 mg X4 mg X5 mg X6 mg X7 mg X8 mg X9 mg X10 mg X mean mg V mean l A found CV found A nominal CV nominal Result A Result CV The t...

Page 18: ...e been carefully cleaned and decontaminated before shipment That the instruments pose no danger through bacteriological chemical radiological or viral contamination That she he is aware that shipment...

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