Operating Manual of Inverted Microscope
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a
①
③
④
⑤
b
c
d
Fig.20
②
3.
Centering of phase contrast device
(1) Remove one eyepiece from the eyepiece tube, and insert the centering eyepiece
②
into the
eyepiece tube, as shown in Fig. 20-a.
(2) A dark ring
④
and a bright ring
⑤
can be observed in the field of view of the centering
eyepiece, as shown in Fig. 20-c.
(3) If the edge of the dark rings and bright rings is unclear in the field of view, the adjusting ring
①
of the centering eyepiece can be adjusted to make the edge clear.
(4) In the phase contrast observation mode, the center of the dark ring should coincide with that of
the bright ring. If their centers do not coincide, as shown in Fig. 20-c, it is necessary to center the
phase contrast. This can be done by adjusting the centering screws
③
of the puller phase contrast.
The adjusted the field of view is as shown in Fig. 20-d.
(5) After the phase contrast device is centered, the centering eyepiece can be removed, and the
working eyepiece inserted again.
Caution
!
In case of changeover to a phase-contrast objective of a different magnification, the
phase-contrast device must be centered again, otherwise the effect of observation
phase-contrast would be affected.
