V&P Scientific, Inc.
7
Technical Note 370B
USING the 850 mL SpinVessel
®
SYSTEM
The optimal stirring mode is dependent upon the application and needs to be empirically
determined.
Factors to consider in determining optimal 850 mL SpinVessel operation are the density
of the particulates, their size and shape, as well as the volume and viscosity of the liquid. Start with
(300 RPM) and 3.1 rotations before reversing direction, then test to determine the appropriate RPMs
and number of rotations for the particulate’s density, fragility, and soluti
on viscosity. We have found
that smaller circumference SpinVessels® require higher RPMs than larger circumference SpinVessels,
because the critical factor is the linear speed generated at the circumference not the RPMs. See page
501 of our
for a full discussion of this phenomenon.
The following settings are ones to use when starting out with the VP 418SV3-1-850RB-CC:
Speed: 300 RPM Rotate: 3.1 times Pause: 200 milliseconds Ramp: 10
It is always a good idea to practice with water in the SpinVessel® to determine safe RPM, rotation,
pause and ramp numbers before using expensive reagents.
The VP 418SV3-1-850CB-CC SpinVessel® is designed to be used with VP 830SV-850CB SpinVessel®
tubes. Please contact V&P Scientific for pricing.
See below for an example of low versus high RPM for a solution of yeast cells in a large circumference,
6-liter SpinVessel®. Note that, when given a longer mixing time, even the slower speed (20 RPM for
more gentle mixing) was able to suspend the yeast cells.
RPM
Fraction of
Rotation
<
300 3, 1, 200, 10 >
Pause
ASCII Command String API For SpinVessel®
Number of
Rotations
Ramping