9. Operation of Kronos
9.1 Start-Up
1.
Switch on the Kronos. The LED will start immediately, and you should be able to see a white
glow inside the enclosure
2.
Start the Kronos software
9.2 Choose Experiment Mode and Set Parameters
1.
Choose Absorption or Emission mode for your experiments
2.
Set the number of times to average and the time window
9.3 Finding the Signal
1.
Place the appropriate excitation and detection filters for your sample. A prior UV/vis absorption
measurement would help determine which are appropriate
2.
Prepare your sample and place it in the Kronos sample holder
9.4 Acquiring Data
1.
Click on the <Run> button and allow the scan to complete
2.
Click on the <Save> button to export data
9.5 Shutdown
1.
Remove the sample and any filters added
2.
Click on the <Exit> button to close the Kronos software
3.
Unplug the USB connector
27