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CyanoFluor U

ser’s Manual 

Rev. A 

15 

 

4.4 External Calibrations to Track Algal Abundance 
 

Algal abundance, or other concentration estimates such as µg/L, can be calculated using 
RFU values via external calibration.  All that is required for an external calibration is a 
standard or sample that has a known concentration and your CyanoFluor.  To externally 
calibrate the CyanoFluor using natural water samples: 
 
1)  Take a grab sample from your aquatic habitat 
2)  Measure the sample using the CyanoFluor and record the RFU value for chlorophyll 
3)  Use extraction methods (EPA method 445.0) to determine the actual chlorophyll µg/L 

concentration of the water sample measured 

4)  Create a conversion factor that converts RFU to µg/L  
 
Now all chlorophyll RFU values can be converted to µg/L concentration estimates using the 
conversion factor created from the grab sample analyzed.   
 
This kind of a correlation can also be established to convert PC RFU values to part per 
billion (ppb) estimates using a PC extraction method or PC pigment which may be ordered 
from 

www.prozyme.com

 and diluted in water to specific concentrations. 

Summary of Contents for CyanoFluor

Page 1: ...P N 998 8701 Revision A February 17 2017 TURNER DESIGNS 1995 N 1st Street San Jose CA 95112 Phone 408 749 0994 FAX 408 749 0998...

Page 2: ...on Check 9 2 4 Filtrate Blank 9 2 5 Sample Analysis Procedure 10 2 6 Filtering Samples for DOM Correction 11 2 7 Data Display 11 2 8 Downloading Data 11 3 Sample Analysis Guidelines 13 4 Measurement P...

Page 3: ...an end of life product proceed as follows If you purchased your instrument through a Turner Designs Distributor please contact your local representative They will instruct you where to return the end...

Page 4: ...production potentially leading to cyanobacterial blooms This monitoring effort can be used to predict the onset of HABs and alert users so the proper protocols can be implemented to prevent or reduce...

Page 5: ...ips includes CyanoFluor inside a storage pouch batteries are installed 1 Glass 10mm x 10mm Square Cuvette 10 Filter Capsules Data Download cable Laminated Quick Start Guide QSG USB Flash Drive with Us...

Page 6: ...Then wipe the inside area dry with a clean soft towel or tissue followed by a quick wipe using non abrasive material such as Kim Wipes If extra cleaning is needed use a mild detergent to dampen a tow...

Page 7: ...selection made If SEND CLEAR DATA is selected press ENTER and a submenu will display Users can choose using keys to either send data to a connected computer or clear logged data a marker will indicate...

Page 8: ...sing the ON OFF button again will turn the unit off or if left idle on the HOME screen for 3 minutes the unit will automatically turn off to save battery power Note CyanoFluor will NOT automatically t...

Page 9: ...t clean use a Kim Wipe to wipe the sides of the cuvette before inserting it into the sample compartment wipe away any moisture and or fingerprints 2 Insert the standard into the CyanoFluor s sample co...

Page 10: ...or fingerprints 5 Press the ON OFF button to turn the instrument on and wait for the 5 second countdown 6 Press the READ button you will be prompted to insert your sample and press read again 7 Making...

Page 11: ...display Measuring Please Wait NOTE See Sample Analysis Procedure section 2 5 step 9 for result displays 2 7 Viewing Data Data can be viewed on the CyanoFluor s display as well as downloaded to a comp...

Page 12: ...anager window 7 Click the Browse button and select or create a folder in a desired directory and enter a filename 8 Click Save and then click the Start button on the software s screen 9 Press the ON O...

Page 13: ...ill help flush out any residual and decrease the chance for cross contamination Do not use a cuvette cap as it may cause the cuvette to not seat properly in the sample compartment When handling the cu...

Page 14: ...chance that a harmful algal bloom will occur 4 2 Turbidity Effects Suspended sediments may decrease accuracy in measurements Coastal marine environments typically have sediment loads between 5 and 10...

Page 15: ...easure the sample using the CyanoFluor and record the RFU value for chlorophyll 3 Use extraction methods EPA method 445 0 to determine the actual chlorophyll g L concentration of the water sample meas...

Page 16: ...ort turnerdesigns com 2 Carry out any adjustments or tests as suggested by the Technical Support Department 3 If proper performance is not obtained you will be issued a Return Authorization number RMA...

Page 17: ...CyanoFluor User s Manual Rev A 17...

Page 18: ...le batteries Auto Power Off After 3 minutes of inactivity on the HOME screen only Light Source LED Detector Photodiode LCD Display 2 x 16 characters Resolution 12 bits Case Meets IP 67 Standard dustpr...

Page 19: ...lear the data log When memory is full the instrument will display Memory Full When READ is pressed you will not be allowed to analyze samples until data have been downloaded and cleared from memory Ba...

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