Turner BioSystems TBS-380 User Manual Download

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User’s Manual

TBS-380

Mini-Fluorometer

Operating Manual

Dated: 08/14/2007

Rev. E

645 N. Mary Avenue

•

Sunnyvale, CA 94085

Phone: (408) 636-2400

•

Toll Free: (888) 636.2401

•

Fax: (408) 737-7919

www.turnerbiosystems.com

Summary of Contents for TBS-380

Page 1: ...User s Manual TBS 380 Mini Fluorometer Operating Manual Dated 08 14 2007 Rev E 645 N Mary Avenue Sunnyvale CA 94085 Phone 408 636 2400 Toll Free 888 636 2401 Fax 408 737 7919 www turnerbiosystems com ...

Page 2: ...For the most current version of this manual visit our Customer Care Center at www turnerbiosystems com ...

Page 3: ...rd 10 4 General Considerations for Analysis 4 1 Handling Samples 11 4 2 Linear Range and Quenching 11 4 3 Temperature Considerations 11 4 4 Positioning Samples 12 4 5 Data Quality 12 5 Troubleshooting 5 1 Low or No Reading 13 5 2 High or Over Reading 13 5 3 Fluctuating Results 13 6 Warranty 6 1 Terms 14 6 2 Warranty Service 14 6 3 Out of Warranty Service 15 Appendices A Instrument Specifications A...

Page 4: ...User Notes and Information Date Notes Information ...

Page 5: ...let and slide it on to the AC adaptor 3 Plug the power supply into the TBS 380 see Section 2 Quick View Diagram Be sure to use only the power supply provided with your instrument by Turner BioSystems Powering the instrument with an unapproved power supply may damage the unit 4 To turn on the TBS 380 press the ON OFF button The instrument takes 5 seconds to warm up After the warm up the TBS 380 is ...

Page 6: ...r Operating Manual 4 2 Quick View Diagram Sample Compartment LCD Screen ON OFF button 10x10 mm Cuvette Holders Minicell Cuvette Holders Serial Port Power Supply Input Positioning the Minicell Adaptor BLUE Position UV Position ...

Page 7: ...ment on and off UP ARROW button STD VAL button Press to set numeric value for the standard solution DATA button Press to access IDL screens Internal Data Logging Fluorescence Reading SET button Press to select sample analysis mode Discrete or Continuous READ button Press to access READ screens CAL button Press to access CALIBRATION screens DOWN ARROW button ENTER button DIAG button Press to access...

Page 8: ...t Press ENTER button five times to begin download If no data is logged the following screen will appear Press ENTER button five times to begin erasing data Erase Data All data erased Press DATA button once for Data Logging status Press DATA button twice to download data Press DATA button three times to erase data Status Stop ENT to toggle Status Logging ENT to toggle Press ENTER button to toggle b...

Page 9: ...n to a PC or printer for continuous mode readings Press ENTER button Continuous Meas Interval 5 sec Press or buttons to change interval time Cont Meas Count 10 100 XX Press or buttons to change number of samples Press ENTER button XXX X UV Press READ button CONT count XX ENT int X sec Press ENTER button Insert Sample Press ENTER Insert sample and press ENTER button READING X XX WAITING X XX XX X X...

Page 10: ...hen finished press the ENTER button to accept the value and return to the HOME screen 3 5 Calibration 1 Press the CAL button 2 Press the ENTER button to start the calibration 3 Insert your blank and press the ENTER button The TBS 380 will average the blank fluorescence for 5 seconds NOTE Be sure to insert the Minicell adaptor in the correct orientation when the Minicell is used see 1 3 5 4 Insert ...

Page 11: ...trument measures and averages the fluorescence signal over a 5 second interval for each sample measurement The instrument measures the sample repeatedly for a user defined sample number 1 Press the SET button 2 Use the UP or DOWN ARROW button to select the Continuous mode 3 Press the ENTER button 4 Use the UP or DOWN ARROW button to select a measurement interval 5 Press the ENTER button 6 Use the ...

Page 12: ...l logged data 4 Press the ESC button when finished to return to the HOME screen 3 8 Diagnostic Information 1 Press the DIAG button to access the DIAGNOSTIC screen 2 The first screen shows the number of data points available for internal data logging 3 Press the ENTER button to toggle to the RFU Relative Fluorescence Units values from the calibration blank and standard 4 Press the ESC button when f...

Page 13: ... and Quenching The linear range is the concentration range in which the readout of the TBS 380 is directly proportional to the concentration of the fluorophore The linear range begins with the smallest detectable concentration and extends to an upper limit concentration that is dependent upon the properties of the fluorescent material the filters used and the path length See the diagram below A no...

Page 14: ...n give slightly different measurements depending upon their orientation in the sample compartment This is due to defects in the shape of the cuvette that are not visible to the human eye We recommend that the cuvette be marked at the top and positioned in the sample compartment the same way each time to minimize error 4 5 Data Quality The TBS 380 is only as accurate as the standards that are used ...

Page 15: ...icell Is the Minicell adaptor inserted in the correct orientation If the BLUE channel is selected insert the Minicell adaptor in the sample compartment with the tab away from you and the BLUE label facing you If UV channel is selected insert the Minicell adaptor in the sample compartment with the tab close to you and the UV label facing you Is the orientation of the Minicell cuvettes consistent se...

Page 16: ...ted by the Technical Support Department 3 If proper the instrument is still not functioning properly you must obtain an RMA number from the Turner BioSystems Service Department before shipping the instrument This number is necessary for tracking and repair under warranty 4 Reference the RMA number on the outside of the package Pack the instrument well as damage incurred during shipping due to impr...

Page 17: ...Proceed exactly as for Warranty Service above If our service department can assist you by phone or correspondence we will be glad to at no charge Repair service will be billed on a basis of time and materials A complete statement of time spent and materials used will be supplied Shipment to Turner BioSystems should be prepaid Your bill will include return shipment freight charges Address for Shipm...

Page 18: ...gnitude ADC Resolution 12 bits LCD Display 2 x 16 characters Operating Temperature 59 98 F 15 40 o C Detectors Photodiodes measurement capability from 300 1000nm Calibration Type Single point and blank Alarms High blank Cuvette Type 10x10 mm plastic 10x10 mm Minicell Adaptor Warm Up Time 5 seconds A2 Optical Specifications Light Source UV LED Blue LED Excitation Optics 365 395 nm 465 485 nm Emissi...

Page 19: ...rt This is usually COM port 2 5 Click on Start The program will find and open MS Excel and should be ready for data transfer The boxes left of the COM port and MS Excel should both be green 6 Follow the directions from Section 3 7 for collecting and downloading data from the TBS 380 Data will automatically appear in the MS Excel spreadsheet Be sure to save this data before closing the software B5 ...

Page 20: ...645 N Mary Avenue Sunnyvale CA 94085 Phone 408 636 2400 Toll Free 888 636 2401 Fax 408 737 7919 www turnerbiosystems com ...

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