THORLABS OTKB Manual Download Page 10

 

Optical Trap Kit 

 

19590-D04-V15; Apr 12, 2012 

Page 10 

www.thorlabs.com 

7)

 

The sample stage consists of a microscope slide holder mounted to a 3-axis (X, Y, and Z) 
translation stage that is mounted on a 1-axis long travel translation stage and to a translating 
breadboard which results in the following capabilities: 

A)

 

2" (50 mm) and 2.4" (60mm) of travel perpendicular to the beam path. This makes it easy 
to load the sample and coarsely position it near the 
trap.  The mounting configuration shown can easily 
be modified to suit other desired translations. 

B)

 

4 mm of travel in the X, Y, and Z directions using the 
modular nanomax differential micrometer drive with 
coarse knobs providing 0.5 mm/rev.  

C)

 

300 µm of travel in the X, Y, and Z directions using 
the differential knobs (50 µm/rev) on the 3-axis 
differential drives.  

D)

 

20 µm of travel in the X, Y, and Z directions using 
the piezo actuators (20 nm resolution without using 
feedback from the internal strain gauge sensors, 5 nm 
resolution using the internal strain gauges for positional feedback) on the 3-axis stage. 
Three T-Cube Piezo Drivers (Item # TPZ001) are included in the kit. (Two T-Cube Strain 
Gauge (Item # TSG001) are included with the OTKBFM force module.) 

 

8)

 

The  visible  light  emitted  from  the  LED  passes  through  the  dichroic  mirror  and  illuminates  the 
sample  while  the  980  nm  laser  beam  is  reflected  towards  the  optional  OTKBFM  Force 
Calibration Module. (If the OTKBFM is not being used, the laser is blocked by a cap, SM1CP2). 
 

9)

 

A single emitter white light LED (Item # LEDWE-15,7.5

o

 half angle forward radiating) is built 

into the component OTKB-LS-SP. The light from the LED illuminates the sample. 
 

10)

 

The  OTKBFM  force  measurement  module  contains  the  hardware  needed  to  calibrate  the  trap. 
The  iris  at  the  back  focal  plane  of  the  objective  (used  as  the  condenser)  is  imaged  on  the 
PDQ80A  Quadrant  Position  Detector  (QPD)  using  a  40  mm  focal  length  biconvex  BK7  lens 
(Item # LB1027-B). The detector is silicon based segmented quadrant position-sensing detector 
with  a  rise  time  of  40  ns  and  a  bandwidth  of  150  kHz.  The  signal  generated  by  the  QPD  is 
sensitive to the relative displacement of the trapped particle from the laser beam axis. As a result 
the output of the detector can be used to calibrate the position, stiffness, and force of the optical 
trap. A TQD001 T-Cube Quadrant Detector Reader is included with this optional force module. 

 

 

 

 

 

Summary of Contents for OTKB

Page 1: ...Optical Trap Kit Manual...

Page 2: ...ety 3 2 Introduction 4 3 Setup Description and Segments 4 4 Optical Trap Kit Components 8 5 Force Measurement Module OTKBFM 11 6 Setup and Alignment in Detail 12 7 Software Package 24 8 Sample prepara...

Page 3: ...this will result in damage to the controllers Set TEC and max LD current parameters according to the specifications of the trapping laser diode before operating the Optical Trapping Kit Failure to do...

Page 4: ...e system allows you to adapt it for a wide variety of applications All of the components needed to construct a fully functional optical trapping system are included in this cost effective kit OTKB con...

Page 5: ...nt the Camera segment and the Vertical segment all connect to this at its three ports as shown in Figure 5 2 Remember to screw the CPB1 to the table as indicated in picture After connecting the Collim...

Page 6: ...eight is right Slide the four ER1 5 rods with vertical post base sliding also into C6W just enough such that the rods don t interfere with the rotation of the B4C in the cube Use the four set screws o...

Page 7: ...gment mainly has the CCD camera for live imaging The imaging lens is already connected to the camera port on the cube C6W To complete this segment just slide the four ER1 rods into the four cage rod h...

Page 8: ...is important to maintaining a constant trapping force 2 The FiberPort Item PAF X 7 B collimates the output of the trapping laser The FiberPort is a versatile collimator since it allows the aspheric c...

Page 9: ...Optical Trap Kit 19590 D04 V15 Apr 12 2012 Page 9 www thorlabs com 5 3 4 8 2 7 9 Figure 3 2 Model of Thorlabs Optical Trapping system 10...

Page 10: ...TPZ001 are included in the kit Two T Cube Strain Gauge Item TSG001 are included with the OTKBFM force module 8 The visible light emitted from the LED passes through the dichroic mirror and illuminates...

Page 11: ...am axis As a result the output of the detector can be used to calibrate the position stiffness and force of the optical trap The detector is connected to the cage cube above the condenser as in Figure...

Page 12: ...to put the Type 1 PCB card into the LM14S2 mount please refer to the LM14S2 manual for details 2 Mount the laser diode in the butterfly controller mount and connect to the laser diode controllers LDC2...

Page 13: ...e desired beam centration is obtained Do not force the screws past their normal operating range if collimation is not easily achieved please contact Tech Support for assistance F Ensure that the beam...

Page 14: ...that external retaining rings on the SM1V10 are on both sides of CP02 Make sure that the lens is mounted in the lens tube so that the collimated beam from the FiberPort is incident on the curved surfa...

Page 15: ...y then you can put segments of the lens cover SC1L24 not included over the two open sections in Figure 6 2 1 You will need a two inch long cut for the first section and a one inch long cut for the sec...

Page 16: ...an iris SM1D12D to this left end port of the C6W Then rotate the B4C on the C6W until the beam is going through the iris when it is almost closed and through the center hole of the VRC4CPT Then use th...

Page 17: ...the KCB1 is used to align the beam through the upper diaphragm Neither should require much adjustment First the lower iris is wide open Then you gradually close this and if necessary use the kinematic...

Page 18: ...from four ER05 cage rods 4 Place an LCP02 on a flat surface and insert the four ER05 cage rods in the 30 mm cage rod holes and secure them at such a level that only one of the two set screws in each...

Page 19: ...adjusters on the HPT1 are oriented as shown in Figure 6 4 2 11 Attach an SM1D12 to the XY translating face of the HPT1 This face has a line marker for each adjuster The condenser will be connected to...

Page 20: ...e slide 17 Lock both C1500 to the DP14 as shown in Figure 6 5 1 18 Use the other two ER6 rods to complete the upper vertical segment as shown in Figure 5 5 1 right 19 If you don t have to install the...

Page 21: ...ssembly is shown in Figure 5 6 1 4 Mount the IR filter FES0750 in an SM1L05 5 Thread in an SM1D12 to the vertical face of the KCB1 then the SM1L05 from step 4 then an SM1T2 and the adapter SM1A9 which...

Page 22: ...immersion objective might push the slide from the bottom during the imaging process the clips are used to hold the slide in place 6 8 The Light Source 1 Remove one half of the SM1Q quick release adap...

Page 23: ...de of the connector on the cable which is marked with a 1 will connect to pin 6 please see LD1255R manual 7 Now gradually turn the Current Control Potentiometer and the LED should light up after a few...

Page 24: ...lane Review of Scientific Instruments 77 103101 2006 The screen shots on the right hand side show the QPD and Strain Gauge Controller software on top included with OTKBFM and the piezo controller soft...

Page 25: ...Following parts will be helpful to setup and operate the trap They are not part of the Optical Trap Kit a Immersion Oil e g Cargille non drying immersion oil for microscopy Type B Cat No 16484 Immers...

Page 26: ...xis Sample Positioning Stage CCD Camera with USB Interface for Video Imaging Position Sensing Detector Module Available Specifications Trap Force 1 pN with Trap Laser Output Power 16 mW Spot Size 1 1...

Page 27: ...4440 Fax 44 0 1353 654444 www thorlabs de email sales uk thorlabs com France Thorlabs SAS 109 rue des C tes 78600 Maisons Laffitte France Tel 33 0 970 444 844 Fax 33 0 811 381 748 www thorlabs de emai...

Page 28: ...Thorlabs Inc 435 Route 206N Newton NJ 07860 USA Phone 973 579 7227 Fax 973 300 3600 www thorlabs com...

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