ThermoFisher Scientific Vanquish VC-D50, Operating Manual

Page: 83 / 188

5 • Installation
Fluorescence Detectors (VC-D50, VC-D51, VF-D50, VF-D51)
Operating Manual Page 83
See also
2
Flow Cells (
}
page 168)
5.7.4 Determining the Backpressure of the Flow Cell
This procedure first describes how to measure the backpressure of the
flow cell (p4) including that of the column, detector inlet capillary and
waste line. Afterward, the backpressure of the column, detector inlet
capillary and the waste line (p1) is subtracted.
Figure 30: Measuring the backpressure of the flow cell including column,
detector inlet capillary and waste line
No. Description No. Description
1 Detector 3 Detector inlet capillary
2 Flow cell 4 Waste container
2a Outlet port of the flow cell 5 Waste line
2b Inlet port of the flow cell
1. Set up the flow connections as described in Flow Connections to the
Flow Cell ( } page 71) .
2. Start the pump flow at the flow rate of your application.
3. Check all flow connections for leakages:
¨
If a leakage has occurred : See Resolving Liquid Leaks
( } page 162) .
¨
If no leakage was found and the pressure value has stabilized :
Proceed with the next step.
4. When the system pressure has stabilized, read the system pressure
in Chromeleon and write down the value for p4.
p4: Pressure drop at the flow cell including the column, detector
inlet capillary and waste line