8.
If the contamination remains, clean the contaminated wells with a 10% bleach
solution.
Repeat step 2 to step 6 with a 10% bleach solution (see “Detailed procedures
for cleaning the sample block” on page 148).
IMPORTANT!
Always use deionized water to rinse wells after cleaning with
bleach or ethanol solution.
9.
Use Smart Help if the contamination remains (see “Request technical support
with Smart Help” on page 159).
Detailed procedures for cleaning the sample block
IMPORTANT!
Use these cleaning procedures
only
in conjunction with the complete
decontamination procedure (see “Clean the sample block” on page 147).
• Rinse the sample block with deionized water.
a.
Pipet a small volume of deionized water into each contaminated well.
b.
In each well, pipet the water up and down several times to rinse the well.
c.
Pipet the water to a waste beaker.
d.
Use a cotton swab to scrub inside of each contaminated well.
e.
Use a lint-free cloth to absorb the excess deionized water.
• Clean the sample block with 95% ethanol.
a.
Pipet a small volume of 95% ethanol solution into each contaminated well.
b.
In each well, pipet the solution up and down several times to rinse the well.
c.
Pipet the ethanol solution to a waste beaker.
IMPORTANT!
Always use deionized water to rinse wells after cleaning with
bleach or ethanol solution.
• Clean the sample block with 10% bleach.
a.
Pipet a small volume of 10% bleach solution into each contaminated well.
b.
In each well, pipet the solution up and down several times to rinse the well.
c.
Pipet the bleach solution to a waste beaker.
IMPORTANT!
Always use deionized water to rinse wells after cleaning with
bleach or ethanol solution.
Chapter 10
Maintain the instrument
Decontaminate the sample block
10
148
QuantStudio
™
6 Pro and 7 Pro Real-Time PCR Systems User Guide
