4
Nucleic Acid Applications
Measure using a Custom Factor
Thermo Scientific
NanoDrop Eight User Guide 25
Measure using a Custom Factor
Measure Nucleic Acid using a Custom Factor
Use the Custom Factor application to quantify purified DNA or RNA samples that
absorb at 260 nm with a user-defined extinction coefficient or factor. The application
reports nucleic acid concentration and two absorbance ratios (A260/A280 and
A260/A230). A single-point baseline correction can also be used.
To measure nucleic acid samples using a custom factor
Before you begin...
Before taking pedestal measurements with the NanoDrop Eight instrument, lift the
instrument arm and clean the upper and lower pedestals. At a minimum, wipe the
pedestals with a new laboratory wipe. For more information, see
To measure using a custom factor
1. From the Home screen, select the
Nucleic Acids
tab and select
Custom
Factor
.
Measures the concentration of
purified nucleic acids using a
custom factor for the
calculations.
NOTICE
• Do not use a squirt or spray bottle on or near the instrument as liquids will
flow into the instrument and may cause permanent damage.
• Do not use hydrofluoric acid (HF) on the pedestals. Fluoride ions will
permanently damage the quartz fiber optic cables.
Summary of Contents for NanoDrop Eight
Page 6: ...Contents vi NanoDrop Eight User Guide Thermo Scientific...
Page 24: ......
Page 56: ...5 Protein Applications Measure Protein A280 56 NanoDrop Eight User Guide Thermo Scientific...
Page 76: ...6 Custom Applications Measure Custom 76 NanoDrop Eight User Guide Thermo Scientific...
Page 142: ...8 Maintenance 142 NanoDrop Eight User Guide Thermo Scientific This page is intentionally blank...
