4
Nucleic Acid Applications
Measure using a Custom Factor
Thermo Scientific
NanoDrop Eight User Guide 29
Detection Limits for Nucleic Acid Measurements using a Custom
Factor
The lower detection limits and reproducibility specifications for nucleic acids are
provided
. The upper detection limits are dependent on the
of the instrument and the user-defined extinction coefficients.
To calculate upper detection limits for nucleic acid samples
To calculate upper detection limits in ng/µL, use the following equation:
(upper absorbance limit
instrumen
t
* extinction coefficient
sample
)
For example, for a sample measurement using an extinction coefficient of 31, the
equation looks like this:
(200 AU * 31 ng-cm/µL) = 6,200 ng/µL
Related Topics
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Summary of Contents for NanoDrop Eight
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