iSort
™
Automated Cell Sorter User Guide
97
Remove and clean
the deflection
plates
The deflection plates must be removed and cleaned on a regular basis to prevent
any buildup of deposits. Significant buildup of sheath fluid deposits due to clogs
or misalignment of the nozzle tip can cause arcing between the deflection plates,
which can damage the instrument.
We recommend removing and cleaning the deflection plates every day after the
daily Shut Down procedure.
DANGER!
ELECTRICAL SHOCK HAZARD.
The deflection plates in the sort
chamber are charged when sorting. A safety interlock on the sort chamber
door disables the deflection plates when the sort chamber door is opened.
Do not disable the safety interlock on the sort chamber door.
1.
Make sure that the instrument is powered off, then open the sort chamber
door to access the deflection plates (see page 11).
2.
Wearing gloves, firmly grab one of the deflection plates and pull it out at a
right angle to remove it from the sort chamber.
Note:
Each deflection plate is secured to the sort chamber by two posts (one
metal, one white plastic) that fit snugly into the holes on the plates. You may
have to apply some force and wiggle the plates as you pull them out.
Sort chamber with deflection plates
Deflection plates removed
3.
Wipe the deflection plates with sterile deionized water to remove dried salt
residue, then wipe dry with Kimwipes
™
laboratory tissues (or other soft, lint
free cloth).
4.
To clean contamination from biological spills, wipe the deflection plates with
Kimwipes
™
tissues moistened with isopropyl alcohol or ethanol, then air dry.
Alternatively, spray the deflection plates with isopropyl alcohol or ethanol
from a spray bottle, then wipe dry with Kimwipes
™
tissues.
IMPORTANT!
The deflection plates must remain clean and dry for optimal
deflection. Before using the iSort
™
Automated Cell Sorter, reinstall the
deflection plates as described on page 22).
Decontaminate the
fluidics lines
To decontaminate the fluidics lines, run the
Sheath Fluidics Cleaning
function.
We recommend that you run the sheath fluidics cleaning procedure at least once
every six months or as needed to decontaminate the fluidics lines. If you plan to
ship your instrument for service, you must also perform the decontamination
procedure. For instructions on how to run Sheath Fluidics Cleaning, see page 60.
Summary of Contents for invitrogen iSort Series
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