22
iSort
™
Automated Cell Sorter User Guide
Install the nozzle
tip
Before starting up the iSort
™
Automated Cell Sorter, you must reinstall the nozzle
tip that has been removed from the nozzle assembly after the last Shut Down
procedure (page 98). For instructions on how to install the nozzle tip on the nozzle
assembly, see page 101.
Install the
deflection plates
If the deflection plates have been removed from the sort chamber after the last Shut
Down (page 97), you must reinstall them before Startup.
1.
Open the sort chamber door in the sorting compartment to access the location
of the deflection plates (page 11).
2.
With the side engraved with “TOP” facing out and on top, place one of the
deflection plates into position so that the white plastic post in the sort chamber
fits through the bottom hole of the deflection plate. The deflection plates are
directional and can only be installed in one way.
3.
Align the top hole of the deflection plate with the top metal post in the sort
chamber, then push the deflection plate firmly until it clicks securely into place.
4.
Repeat the installation process for the other deflection plate.
Deflection plates removed
Deflection plates reinstalled
IMPORTANT!
The deflection plates must be removed and cleaned on a regular
basis to prevent any buildup of deposit (page 97). Significant buildup of sheath
fluid deposits due to clogs or misalignment of the nozzle tip can cause arcing
between the deflection plates, which can damage the instrument.
Clean the sort
chamber and the
sample input port
Before running a sort or analysis, clean the sample input port and the sort chamber,
including the sort drawer, door, sort window, and deflection plates (which can be
removed). To clean the instrument surfaces, you can use Kimwipes
™
laboratory
tissues (or other soft, lint free cloth). Do
not
use abrasive cloths or sponges and
harsh or corrosive cleansers.
IMPORTANT!
Do
not
attempt to clean the lenses inside the laser interrogation
chamber. This should only be done by trained service professionals.
1.
Wipe the sort drawer, the door, the stream watch camera window, and the
deflection plates with sterile deionized water to remove dried salt residue, then
wipe dry with Kimwipes
™
tissues.
Note:
The deflection plates must remain clean and dry for optimal deflection.
2.
Blot any liquid from the nozzle tip, the floor of the sort chamber, and the
sample input port with Kimwipes
™
tissues.
Summary of Contents for invitrogen iSort Series
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