.
1 July 2019
-5-
E-Base
™
protein electrophoresis protocol
Step
Action
14–30 min
4
Run the gel
a. Select the EP program (default run time 14 minutes) for running E-PAGE
™
cassettes by
pressing and releasing the “pwr/prg”.
b. Select the recommended run time for a specific gel type by pressing and releasing the time
button, then press and hold the time button to increase the time. Release time button when
the desired run time for the gel is reached.
Gel type
Recommended run time
Maximum run time
E-PAGE
™
48
gel
25 min
30 min
E-PAGE
™
96
gel
14 min
25 min
c. Start the run by pressing and releasing the “pwr/prg” button. The red indicator light will
change to green.
5
End the run
a. A flashing red indicator light and rapid beeping indicates the end of the run. Press and
release “pwr/prg” to stop the device.
b. For better detection sensitivity, allow the gel to cool down for 10 minutes after the end of
the run.
1–2 hr
6
Stain the gel
a. Open the gel cassette.
b. Visualize the protein by staining the gel using any of the following techniques (See the
for details on staining and imaging).
·
SYPRO
™
Ruby Protein Gel Stain protocol
·
Coomassie R-250 protocol
·
SimplyBlue
™
SafeStain protocol
·
SilverQuest
™
Silver Stain protocol
·
SilverXpress
™
Silver Stain protocol
