Touchdown PCR Run Method
Use this run method if the optimal annealing temperature is not known. This method incrementally
decreases the annealing temperature in early cycles to maximize the yield of specific products.
XL PCR Run Method
Use this run method for amplification of 5 to 40 kb PCR products using
rTth
DNA Polymerase, XL
and unique reaction conditions. By providing longer templates, XL PCR complements technologies for
rapid, long-range PCR. More complete genes can be amplified in one reaction from known expressed
sequences, allowing more introns to be spanned. You can use XL PCR to amplify the control target (a
20.8
‑
kb product from Lambda DNA) supplied in the kit.
Limited product warranty
Life Technologies Corporation and/or its affiliate(s) warrant their products as set forth in the
Life Technologies' General Terms and Conditions of Sale at
www.thermofisher.com/us/en/home/
global/terms-and-conditions.html
. If you have any questions, please contact Life Technologies at
Appendix E
Predefined run methods
Limited product warranty
E
96
Veriti
™
Thermal Cycler User Guide
