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ThermoFisher Scientific 75003602, Instruction Manual

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Page 1: ...Thermo Scientific Microliter 48 x 2 sealed Instruction Manual 50120031 e 05 2020 Visit us online to register your warranty thermofisher com labwarranty ...

Page 2: ...WEEE Conformity This product is subject to the regulations of the EU Waste Electrical Electronic Equipment WEEE Directive 2012 19 EU It is marked by the following symbol ...

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Page 5: ...Hz refrigerated 1 9 100 V 50 60 Hz ventilated 1 10 100 V 50 60 Hz refrigerated 1 11 230 V 50 60 Hz ventilated 1 12 230 V 50 60 Hz refrigerated 1 12 120 V 60 Hz ventilated 1 13 120 V 60 Hz refrigerated 1 13 Chapter 2 Accessories 2 1 Chapter 3 AutoLock 3 1 Rotor Installation 3 2 Removing the Rotor 3 3 Aerosol tight rotors 3 3 Chapter 4 Rotor Loading 4 1 Before Run 4 2 Proper Loading 4 2 Improper Loa...

Page 6: ...2 sealed Thermo Scientific Chapter 6 Maintenance and Care 6 1 Cleaning intervals 6 2 Cleaning 6 2 Disinfection 6 3 Decontamination 6 4 Autoclaving 6 5 Service of Thermo Fisher Scientific 6 6 RCF Values A 1 Chemical Compatibility Chart B 1 ...

Page 7: ...will result in the expiration of the seller s warranty Scope of Supply If any parts are missing please contact the nearest Thermo Fisher Scientific representative Precautions In order to ensure safe operation of the Microliter 48 x 2 sealed the following general safety regulations must be followed Do not remove the magnet at the rotor bottom Do not use rotors which show any signs of corrosion and ...

Page 8: ...lease pay particular attention to the following aspects Rotor installation Check that the rotor is locked properly into place before operating the centrifuge Always balance the samples Maximum sample density at maximum speed 1 2 g ml This symbol refers to general hazards ATTENTION means that material damage could occur WARNING means that injuries or material damage or contamination could occur Thi...

Page 9: ...Thermo Scientific 75003602 Microliter 48 x 2 sealed 1 1 1 Rotor Specifications Contents Technical Data on page 1 2 ...

Page 10: ... 45 45 Accel braking time s 45 45 45 45 45 45 Sample heating at nmax C referred to ambient temperature of 23 C running time 120 minutes 19 19 16 Aerosol tight Tested by HPA Porton down UK yes yes yes Permissible temperature range autoclavable C 121 121 121 Centrifuge Megafuge 40 Catalog 75004503 Weight empty kg 2 4 Max cycle number 50 000 Maximum permissible load g 48 x 4 Maximum speed nmax rpm 15...

Page 11: ... value at nmax 25 314 25 314 25 314 max min radius cm 9 8 5 9 9 8 5 9 9 8 5 9 Pitch angle 45 45 45 Accel braking time s 45 45 45 45 45 45 aerosol tight yes yes yes permissible temperature range autoclavable C 121 121 121 Tested by HPA Porton down UK Centrifuge Megafuge 40R Catalog 75004518 Weight empty kg 2 4 max cycle number 50 000 Maximum permissible load g 48 x 4 maximum speed nmax rpm 15 200 m...

Page 12: ...cel braking time s 45 45 45 45 55 50 Sample heating at nmax C referred to ambient temperature of 23 C running time 120 minutes 19 19 16 Aerosol tight Tested by HPA Porton down UK yes yes yes Permissible temperature range autoclavable C 121 121 121 Centrifuge Megafuge 40 Catalog 75004518 Weight empty kg 2 4 Max cycle number 50 000 Maximum permissible load g 48 x 4 Maximum speed nmax rpm 15 200 Maxi...

Page 13: ...alue at nmax 25 314 25 314 25 314 Max min radius cm 9 8 5 9 9 8 5 9 9 8 5 9 Pitch angle 45 45 45 Accel braking time s 45 45 45 45 55 50 Aerosol tight yes yes yes Permissible temperature range autoclavable C 121 121 121 Tested by HPA Porton down UK Centrifuge Megafuge 40R Catalog 75004519 Weight empty kg 2 4 Max cycle number 50 000 Maximum permissible load g 48 x 4 Maximum speed nmax rpm 15 200 Max...

Page 14: ...5 45 Accel braking time s 45 45 45 45 45 45 Sample heating at nmax C referred to ambient temperature of 23 C running time 120 minutes 19 19 16 Aerosol tight Tested by HPA Porton down UK yes yes yes Permissible temperature range autoclavable C 121 121 121 Centrifuge Sorvall ST 40 Catalog 75004509 Weight empty kg 2 4 Max cycle number 50 000 Maximum permissible load g 48 x 4 Maximum speed nmax rpm 15...

Page 15: ...m RCF value at nmax 25 314 25 314 25 314 Max min radius cm 9 8 5 9 9 8 5 9 9 8 5 9 Pitch angle 45 45 45 Accel braking time s 45 45 45 45 45 45 Aerosol tight yes yes yes Permissible temperature range autoclavable C 121 121 121 Tested by HPA Porton down UK Centrifuge Sorvall ST 40R Catalog 75004524 Weight empty kg 2 4 Max cycle number 50 000 Maximum permissible load g 48 x 4 Maximum speed nmax rpm 1...

Page 16: ... 45 Accel braking time s 45 45 45 45 55 50 Sample heating at nmax C referred to ambient temperature of 23 C running time 120 minutes 19 19 16 Aerosol tight Tested by HPA Porton down UK yes yes yes Permissible temperature range autoclavable C 121 121 121 Centrifuge Sorvall ST 40 Catalog 75004510 Weight empty kg 2 4 Max cycle number 50 000 Maximum permissible load g 48 x 4 Maximum speed nmax rpm 15 ...

Page 17: ...RCF value at nmax 25 314 25 314 25 314 Max min radius cm 9 8 5 9 9 8 5 9 9 8 5 9 Pitch angle 45 45 45 Accel braking time s 45 45 45 45 55 50 Aerosol tight yes yes yes Permissible temperature range autoclavable C 121 121 121 Tested by HPA Porton down UK Centrifuge Sorvall ST 40R Catalog 75004525 Weight empty kg 2 4 Max cycle number 50 000 Maximum permissible load g 48 x 4 Maximum speed nmax rpm 15 ...

Page 18: ...45 45 Accel braking time s 45 45 45 45 60 50 Sample heating at nmax C referred to ambient temperature of 23 C running time 120 minutes 19 19 21 Aerosol tight Tested by HPA Porton down UK yes yes yes Permissible temperature range autoclavable C 121 121 121 Centrifuge Sorvall ST 40 Catalog 75004511 Weight empty kg 2 4 Max cycle number 50 000 Maximum permissible load g 48 x 4 Maximum speed nmax rpm 1...

Page 19: ...m RCF value at nmax 25 314 25 314 25 314 Max min radius cm 9 8 5 9 9 8 5 9 9 8 5 9 Pitch angle 45 45 45 Accel braking time s 45 45 45 45 60 60 Aerosol tight yes yes yes Permissible temperature range autoclavable C 121 121 121 Tested by HPA Porton down UK Centrifuge Sorvall ST 40R Catalog 75004526 Weight empty kg 2 4 Max cycle number 50 000 Maximum permissible load g 48 x 4 Maximum speed nmax rpm 1...

Page 20: ...e heating at nmax C referred to ambient temperature of 23 C running time 120 minutes 19 19 Aerosol tight Tested by HPA Porton down UK yes yes Permissible temperature range autoclavable C 121 121 Table 1 12 230 V 50 60 Hz refrigerated Centrifuge SL 40R SL 40 FR Catalog 75004527 75004543 Weight empty kg 2 4 2 4 Max cycle number 50 000 50 000 Maximum permissible load g 48 x 4 48 x 4 Maximum speed nma...

Page 21: ...5 45 Sample heating at nmax C referred to ambient temperature of 23 C running time 120 minutes 19 Aerosol tight Tested by HPA Porton down UK yes Permissible temperature range autoclavable C 121 Table 1 14 120 V 60 Hz refrigerated Centrifuge SL 40R Catalog 75004528 Weight empty kg 2 4 Max cycle number 50 000 Maximum permissible load g 48 x 4 Maximum speed nmax rpm 15 200 Maximum RCF value at nmax 2...

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Page 23: ...Thermo Scientific 75003602 Microliter 48 x 2 sealed 2 1 2 Accessories Contents Rotor data on page 2 2 Accessories on page 2 2 ...

Page 24: ... Description Cat No Qty Pkg No of Places 2 Microtube 11 x 45 1 5 Microtube 11 x 45 1 5 Millipore Ultra MC 11 x 50 0 6 Microtube 7 x 32 76003758 12 1 0 4 Microtube 7 x 29 76003759 12 1 0 2 PCR Reaction Tube 6 x 24 76003750 12 1 Tube Vol ml Vol Fill ml Description Qty Max Speed rpm Microliter 48 x 2 Fixed Angle Aluminium Sealed Rotor 45 15 200 Angle o Maximum Speed rpm 556 2 4 Dims Ø x L mm Capacity...

Page 25: ...Thermo Scientific 75003602 Microliter 48x2 sealed 3 1 3 AutoLock Contents Rotor Installation on page 3 2 Removing the Rotor on page 3 3 ...

Page 26: ...entrifuge spindle The rotor clicks automatically into place 3 Check if the rotor is properly installed by lifting slightly on the handle If the rotor can be pulled up then it must be reclamped to the centrifuge spindle CAUTION Unapproved or incorrectly combined accessories can cause serious damage to the centrifuge CAUTION Do not force the rotor onto the centrifuge spindle If the rotor is very lig...

Page 27: ...h both hands and remove it from the centrifuge spindle Make sure not to jam the rotor while doing this Figure 3 2 Handling of AutoLock Aerosol tight rotors CAUTION Check that the rotor is properly locked on the centrifuge spindle before each use by pulling its handle Be sure to check all seals before starting any aerosol tight applications CAUTION Rotors supplied with a lid for aerosol tight appli...

Page 28: ...3 AutoLock Aerosol tight rotors 3 4 75003602 Microliter 48 x 2 sealed Thermo Scientific Figure 3 3 AutoLock lid for aerosol tight rotors WARNING Mind the AutoLock mandril inside the lid Do not touch ...

Page 29: ...Thermo Scientific 75003602 Microliter 48 x 2 sealed 4 1 4 Rotor Loading Contents Before Run on page 4 2 Proper Loading on page 4 2 Improper Loading on page 4 3 Maximum Loading on page 4 3 ...

Page 30: ... use 2 Check the rotor and all accessory parts for damages such as cracks scratches or traces of corrosion 3 Check the rotor chamber the centrifuge spindle and the AutoLock 4 Check the rotor s suitability using the chemical compatibility chart on page B 1 5 Make sure the tubes or bottles do not touch the lid Proper Loading To ensure safe operation of the centrifuge the rotor must be evenly loaded ...

Page 31: ...reserve stability even when spinning at top speed The safety system of the centrifuge requires that you do not overload the rotor There are two options available for centrifuging samples whose weight including adapter exceeds the maximum permissible load Reduce the fill level Reduce the speed Use the table or the formula Actual Load g cavity RPM max 2 15200 2 2 14493 2 4 13876 2 6 13331 ...

Page 32: ...rotor table in the rotor specification section The maximum number of cycles for buckets is marked on the buckets themselves Service life examples 2 8 12846 3 12411 3 2 12017 3 4 11658 3 6 11329 3 8 11027 4 10748 Actual Load g cavity RPM max nadm nmax Maximum permissible load Effective load WARNING Replace the rotor when the specified number of cycles is reached Due to the mechanical load a rotor c...

Page 33: ...Thermo Scientific 75003602 Microliter 48 x 2 sealed 5 1 5 Aerosol tight Applications Contents Basic Principles on page 5 2 Fill Level on page 5 2 Checking the Aerosol Tightness on page 5 2 ...

Page 34: ...brittlement are extremely important Aerosol tight applications are not possible if the lids are open Aerosol tightness requires the correct operation when filling the sample vessels and closing the rotor lid As a quick test it is possible to test the aerosol tightness of fixed angle rotors using the following process 1 Lubricate all seals lightly Always use the special grease 76003500 when lubrica...

Page 35: ...he test 5 Dry the rotor rotor lid and the cover seal CAUTION Prior to each use the seals in the rotor are to be inspected in order to assure that they are correctly seated and are not worn or damaged Damaged seals are to be replaced immediately When loading the rotor ensure that the rotor lid closes securely Damaged or clouded rotor covers are to be replaced immediately ...

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Page 37: ...oliter 48 x 2 sealed 6 1 6 Maintenance and Care Contents Cleaning intervals on page 6 2 Cleaning on page 6 2 Disinfection on page 6 3 Decontamination on page 6 4 Autoclaving on page 6 5 Service of Thermo Fisher Scientific on page 6 6 ...

Page 38: ...me of the parts Use only disinfectants with a pH of 6 8 Dry aluminum parts off with a soft cloth After cleaning treat the entire surface of aluminum parts with corrosion protection oil 70009824 Also treat the cavities with oil Store the aluminum parts at room temperature or in a cold storage room with the cavities pointing down Maintenance Recommended interval Clean rotor chamber Daily or when pol...

Page 39: ...nfectious material has spilled during centrifugation Use a sprayer whenever possible so that all surfaces are covered evenly The rotor chamber and the rotor should be treated preferably with a neutral disinfectant A disinfectant spray would be most suitable for this purpose so that the rotor and accessory surfaces are covered evenly CAUTION When cleaning do not allow liquids especially organic sol...

Page 40: ...th or in a warm air cabinet at a maximum temperature of 50 C 11 After cleaning treat the entire surface of aluminum parts with corrosion protection oil 70009824 Also treat the cavities with oil 12 Treat the bolt of the swing out rotor with bolt grease 75003786 Decontamination Decontaminate the centrifuge immediately whenever radioactive material has spilled during centrifugation For general radioa...

Page 41: ...aning with a cloth or in a warm air cabinet at a maximum temperature of 50 C 11 After cleaning treat the entire surface of aluminum parts with corrosion protection oil 70009824 Also treat the cavities with oil 12 Treat the bolt of the swing out rotor with bolt grease 75003786 Autoclaving 1 Before autoclaving clean the rotor and accessories as described above 2 Place the rotor on a flat surface Rot...

Page 42: ...ecommends having the centrifuge and accessories serviced once a year by an authorized service technician The service technicians check the following the electrical equipment the suitability of the set up site the lid lock and the safety system the rotor the fixation of the rotor and the drive shaft Thermo Fisher Scientific offers inspection and service contracts for this work ...

Page 43: ...00 5 9 9 8 66 110 1100 5 9 9 8 80 133 1200 5 9 9 8 95 158 1300 5 9 9 8 111 185 1400 5 9 9 8 129 215 1500 5 9 9 8 148 247 1600 5 9 9 8 169 280 1700 5 9 9 8 191 317 1800 5 9 9 8 214 355 1900 5 9 9 8 238 396 2000 5 9 9 8 264 438 2100 5 9 9 8 291 483 2200 5 9 9 8 319 530 2300 5 9 9 8 349 580 2400 5 9 9 8 380 631 2500 5 9 9 8 412 685 2600 5 9 9 8 446 741 2700 5 9 9 8 481 799 2800 5 9 9 8 517 859 2900 5...

Page 44: ...277 2121 4500 5 9 9 8 1336 2219 4600 5 9 9 8 1396 2318 4700 5 9 9 8 1457 2420 4800 5 9 9 8 1520 2524 4900 5 9 9 8 1584 2631 5000 5 9 9 8 1649 2739 5100 5 9 9 8 1716 2850 5200 5 9 9 8 1784 2963 5300 5 9 9 8 1853 3078 5400 5 9 9 8 1923 3195 5500 5 9 9 8 1995 3314 5600 5 9 9 8 2069 3436 5700 5 9 9 8 2143 3560 5800 5 9 9 8 2219 3686 5900 5 9 9 8 2296 3814 6000 5 9 9 8 2375 3944 6100 5 9 9 8 2454 4077 ...

Page 45: ... 7188 8200 5 9 9 8 4435 7367 8300 5 9 9 8 4544 7548 8400 5 9 9 8 4654 7731 8500 5 9 9 8 4766 7916 8600 5 9 9 8 4879 8103 8700 5 9 9 8 4993 8293 8800 5 9 9 8 5108 8485 8900 5 9 9 8 5225 8679 9000 5 9 9 8 5343 8875 9100 5 9 9 8 5462 9073 9200 5 9 9 8 5583 9273 9300 5 9 9 8 5705 9476 9400 5 9 9 8 5828 9681 9500 5 9 9 8 5953 9888 9600 5 9 9 8 6079 10097 9700 5 9 9 8 6206 10309 9800 5 9 9 8 6335 10523 ...

Page 46: ... 5 9 9 8 9341 15515 12000 5 9 9 8 9499 15777 12100 5 9 9 8 9657 16041 12200 5 9 9 8 9818 16308 12300 5 9 9 8 9979 16576 12400 5 9 9 8 10142 16847 12500 5 9 9 8 10307 17119 12600 5 9 9 8 10472 17394 12700 5 9 9 8 10639 17672 12800 5 9 9 8 10807 17951 12900 5 9 9 8 10977 18233 13000 5 9 9 8 11148 18516 13100 5 9 9 8 11320 18802 13200 5 9 9 8 11493 19090 13300 5 9 9 8 11668 19381 13400 5 9 9 8 11844 ...

Page 47: ...14300 5 9 9 8 13489 22405 14400 5 9 9 8 13678 22719 14500 5 9 9 8 13869 23036 14600 5 9 9 8 14060 23355 14700 5 9 9 8 14254 23676 14800 5 9 9 8 14448 23999 14900 5 9 9 8 14644 24324 15000 5 9 9 8 14841 24652 15100 5 9 9 8 15040 24982 15200 5 9 9 8 15240 25314 Speed rpm Rmin Rmax RCF Rmin RCF Rmax ...

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Page 49: ... U U S S S S U S S S S M S S S S S S S S S M U U S S Formic Acid 100 S M U U U S M U U S S U S U S U Ammonium Acetate S S U S S S S S S S S S S U S S S S S S S S S S Ammonium Carbonate M S U S S S S S S S S S S S U U S S S S S S M S S S Ammonium Hydroxide 10 U U S U S S M S S S S S S U M S S S S S S S S S M S Ammonium Hydroxide 28 U U S U S U M S S S S S U S U M S S S S S S S S S M S Ammonium Hydr...

Page 50: ...U S S S M S S S S S S Dimethylsulfoxide S S U U S S S S U S S U S U U S S U U S S S S U U Dioxane M S U U S S M M S U U S U M U U M M M U S S S S U U Ferric Chloride U U S M S M S S S S M U S S Acetic Acid Glacial S S U U S S U M S U S U U U U U M S U M U S U U S U Acetic Acid 5 S S M S S S M S S S S S M S S S S S S S M S S M S S M Acetic Acid 60 S S U U S S U S M S U U M U S M S M S M S M U S M U...

Page 51: ...sium Hydroxide conc U U M U M M S S U M U U U S M M U U U U Potassium Permanganate S S S S S S S S S U S S S M S M S U S S M S U S Calcium Chloride M U S S S S S S S S S S S S M S S S S S S S M S S S Calcium Hypochlorite M U S M M S M S S M S S S S M S M U S S Kerosene S S S S S S U S M U S U M M S M M M S S U S S U S Sodium Chloride 10 S S S S S S S S S S S S S S S S S S M S Sodium Chloride sat d...

Page 52: ...e S S S S S S S M S S S S S S M S S S S S S S S S S Nickel Salts U S S S S S S S S S S S S S S S S S S M S S S Oils Petroleum S S S S U S S S S U U M S M U U S S S U S S S S Oils Other S S S M S S S S U S S S S U S S S S S S M S Oleic Acid S U S S S U U S U S S M S S S S S S S S S M U S M M Oxalic Acid U U M S S S U S S S S S U S U S S S S S S S S U M S S Perchloric Acid 10 U U S U U S M M M U M S...

Page 53: ... S S U U Toluene S S U U S S M U S U U S U U U S U M U U U S U S U U M Trichloroacetic Acid U U U S S U M S U S U U S M M S S U U S U U U M U Trichloroethane S U M U U S U U U U U U U U U S U S S Trichloroethylene U U U U S U U U U U U U U U S U U S Trisodium Phosphate S M S S S S S S S Tris Buffer neutral pH U S S S S S S S S S S S S S S S S S S S S S S S S S Triton X 100 S S S S S S S S S S S S ...

Page 54: ...a exists for materials under the stress of centrifugation when in doubt we recommend pretesting sample lots Key S Satisfactory M Moderate attack may be satisfactory for use in centrifuge depending on length of exposure speed involved etc suggest testing under actual conditions of use U Unsatisfactory not recommended Performance unknown suggest testing using sample to avoid loss of valuable materia...

Page 55: ... Checking the Aerosol Tightness 5 2 Cleaning Agents 6 2 Contact Information D 1 Cycle Counter 4 4 D Decontamination 6 4 Disinfection 6 2 F Fill Level 5 2 I Improper Loading 4 3 Intervals 6 2 M Maintenance 6 1 Maximum Loading 4 3 P Precautions v Preface v Proper Loading 4 2 Q Quick Test 5 2 R Removing the Rotor 3 3 Rotor Installation 3 2 Rotor Loading 4 1 S Scope of Supply v Service 6 5 Index ...

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Page 57: ...terms and pricing are subject to change Not all products are available in all countries Please consult your local sales representative for details Shown pictures within the manual are examples and may differ considering the set parameters and language Pictures of the user interface within the manual are showing the English version as example Australia 61 39757 4300 Austria 43 1 801 40 0 Belgium 32...

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