5
Aerosol-tight Applications
Basic Principles
5-2
75003602 Microliter 48
x
2 sealed
Thermo Scientific
Basic Principles
•
Check that the sample containers are well suited for the desired centrifugation process.
Fill Level
The tubes are only to be filled to a level which ensures that the sample is unable to reach the top of
the tube during centrifugation. Therefore
,
fill the tube only 2/3 of the rated level.
Checking the Aerosol-Tightness
Quick Test
The aerosol tightness testing of the rotors and buckets depend on the microbiological test process in
accordance with the EN 61010-2-020 Appendix AA.
Whether or not a rotor is aerosol-tight
,
depends primarily on proper handling.
Check as needed to make sure your rotor is aerosol-tight.
The careful inspection of the seals and seal surfaces for signs of wear and damage such as cracks,
scratches and embrittlement
are
extremely important.
Aerosol-tight applications are not possible if the lids are open.
Aerosol-tightness requires the correct operation when filling the sample vessels and closing the rotor
lid.
As a quick test, it is possible to test the aerosol-tightness of fixed-angle rotors using the following
process:
1.
Lubricate all seals lightly
.
Always use the special grease 76003500 when lubricating the seals
.
2.
Fill the cavities with approx. 10 ml of carbonated mineral water
.
3.
Close the rotor as explained in the handling instructions
.
4.
Shake the rotor vigorously using your hands
.
This releases the carbonic acid gas
,
which is bound in the water, resulting in excess pressure.
Do
not apply pressure to the lid when doing so
.
Leaks can be detected by escaping water or the sound of escaping gas
.
CAUTION
When centrifuging hazardous samples, do not open aerosol-tight rotors or
buckets unless placed in a safety cabinet.
Always bear in mind the maximum permitted fill levels.
Be sure to check all seals before starting any aer
o
sol-tight applications.
