Sterilization Cycle
Information You Need to Know Before Starting a Cycle
• If the Sterilization Cycle is to be performed on initial start-up with new unused filters, do
not install the access port or HEPA filters until AFTER the Sterilization Cycle. These fil-
ters should be sterilized only to decontaminate them for disposal. The filters’ ability to
capture particulates may be degraded after sterilization.
• The Sterilization Cycle requires approximately 14 hours -
heat-up (2.5-5 hours), sterilize (2 hours), and cool down (8-10 hours).
Additional time is needed to verify the calibration of temperature and CO
2
after the cycle is complete.
• During the Sterilization Cycle, the incubator updates the temperature to the analog out-
put board and the 1535, however CO
2
and RH will be fixed at setpoint.
• A HEPA filter replacement kit (see the spare parts list) should be on hand
prior to initiating the Sterilization Cycle.
Information About the Cycle
• Pre-cleaning may be required. In order to avoid odors, stains on the interior, baked-on
material, etc., wipe off all visible signs of spills.
• Odor may occur during the Sterilization Cycle and is considered normal.
• The Sterilization Cycle is not intended to sterilize other items; instruments, etc., from the
lab.
• During the cycle, the unit chamber becomes hot enough to melt samples, instruments,
dishes, etc., left inside the unit. The RH and CO
2
sensors also need to be removed.
• During the Sterilization Cycle, discoloration of some materials may occur. For example,
stainless steel turns a straw color after a period of exposure to high temperatures. This
is normal.
Steri-Cult Incubator ______________________________________________________________________Sterilization Cycle
8 - 1
Section 8 - Sterilization Cycle
