Models:
3307/3308 and 3310/3311
Steri-Cult CO
2
Incubator
Controlled RH with Sterilization Cycle
Operating and Maintenance Manual
Manual No: 7013307 Rev. 3
Page 1: ...Models 3307 3308 and 3310 3311 Steri Cult CO2 Incubator Controlled RH with Sterilization Cycle Operating and Maintenance Manual Manual No 7013307 Rev 3 ...
Page 2: ...orporation makes no representa tions or warranties with respect to this manual In no event shall Thermo be held liable for any damages direct or incidental aris ing out of or related to the use of this manual MANUAL NUMBER 7013307 4 11 05 Added heat CO2 information pertaining to door openings in Section 5 7 ccs 3 22767 IN 3242 3 9 05 Corrected RH sensor from 290154 to 290174 electrical schematics ...
Page 3: ... qualified persons should perform procedures associated with this symbol Hot surface s present which may cause burns to unprotected skin or to materials which may be damaged by elevated temperatures Always use the proper protective equipment clothing gloves goggles etc Always dissipate extreme cold or heat and wear protective clothing Always follow good hygiene practices Each individual is respons...
Page 4: ...Steri Cult Incubator ___________________________________________________________________________Service iii ...
Page 5: ...l can provide precise calibrations as a billable service at your location Calibration after a warranty repair is covered under the warranty Damage resulting from use of improper quality water chemicals or cleaning agents detrimental to equipment materials Service calls for improper installation or operating instructions Corrections to any of the following are billable services 1 electrical service...
Page 6: ...1 d Setting an Access Code 4 1 e Setting a Low Temperature Alarm Limit 4 2 f Enabling Temperature Alarms to Trip Contacts 4 2 g Setting a Low CO2 Alarm Limit 4 2 h Setting a High CO2 Alarm Limit 4 2 i Enabling CO2 Alarms to Trip Contacts 4 2 j Setting a Low RH Alarm Limit 4 2 k Setting a High RH Alarm Limit 4 3 l Enabling RH Alarms to Trip Contacts 4 3 m Selecting a Primary Tank w Gas Guard Option...
Page 7: ...net plate will not withstand the heat of the sterilization cycle Simply unhook the whole plate from the outer door and set aside before initiating the cycle then reinstall afterward 1 3 Control Panel Keys Displays and Indicators Figure 1 2 1 Silence Silences the audible alarm 2 Alarm Indicator Light pulses on off during an alarm condition in the cabinet 3 Mode Select Switch Used to select Run Setp...
Page 8: ...onditions with the HEPA filter installed the air inside the chamber meets the Class 100 air cleanliness standard for particulates of 0 5 micron size or larger per cubic foot of air For further information on the Class 100 classification of air quality see Appendix A 3 Upper Displays The first upper display shows the tem perature The second display shows the percentage of CO2 The third display show...
Page 9: ... on each side with the tab facing into the center of the chamber with their slots up Press downward on the top section of each pilaster to locate then release when in position Figure 1 8 Fit each pilaster securely into the indentations at each side of the ceiling and the floor of the chamber Figure 1 6 shows the diffuser pan pilasters and shelf channels Note that there is a difference between the ...
Page 10: ...sing cau tion not to touch the filter media 3 Loosen black wingnuts in front corners of the chamber ceiling Figure 1 10 1 11 4 Locate the metal lip at the upper back of the chamber Hook the HEPA filter carefully over the back edge of the metal lip 5 Lift the front of the filter to the ceiling of the chamber Push filter bracket back over the filter frame Tighten the wingnuts 6 Refer to Section 6 4 ...
Page 11: ...de in any eight hour shift of a 40 hour work week shall not exceed the eight hour time weighted average of 5000 PPM 0 5 CO2 The short term exposure limit for 15 minutes or less is 30 000 ppm 3 CO2 Carbon dioxide monitors are recommended for confined areas where concentrations of carbon dioxide gas can accumulate This incubator is designed to be operated with CO2 gas only Connecting a flammable or ...
Page 12: ...ure that the connections are secured with clamps Check all fittings for leaks For units having the CO2 Gas Guard option refer to Section 8 2 k Humidity and Loading the Chamber With the chamber loaded with liquid product and the RH setpoint at less than 90 some evaporation of product will occur If the actual RH reaches 2 above setpoint the incuba tor microprocessor turns on dehumidification This pr...
Page 13: ...is set above the overtemp setpoint the overtemp setpoint will auto matically update to 1 C above the temperature setpoint 2 1 It is recommended that the overtemp setpoint be main tained at 1 C over the operating temperature setpoint To change the Overtemp setpoint 1 Press the Mode key until the Settings indicator lights 2 Press the right arrow until OVERTEMP XX XC is dis played in the message cent...
Page 14: ...Steri Cult Incubator ______________________________________________________________________Incubator Settings 2 2 ...
Page 15: ...he Mode key to return to Run or the right left arrow to go to next previous parameter 3 1 b Calibrating the Infrared CO2 System All models of the Steri Cult incubator have an infrared IR CO2 sensor Infrared CO2 sensors are not effected by chamber atmosphere temperature or humidity CO2 Sensor Stabilization Times Start Up Allow the CO2 of the cabinet to stabilize at least 12 hours before proceeding ...
Page 16: ...Steri Cult Incubator ________________________________________________________________________Calibration 3 2 ...
Page 17: ... access code 4 Press Enter to save the access code 5 Press the Mode key to return to the Run mode or right left to go to next previous parameter Section 4 Configuration 4 1 Configuration Mode Several features available in the Configuration Mode allow custom setup of the incubator These features are listed and described below All features may not be necessary in all appli cations but are available ...
Page 18: ...ow temperature alarm limit is the deviation from the temperature setpoint which causes a low temp alarm The low temp alarm is variable from one half degree to five degrees below setpoint The factory setting is 1 below setpoint A minus sign in the display indicates that the alarm setting is below the setpoint 1 Press the Mode key until the Configuration indicator lights 2 Press the right arrow unti...
Page 19: ...tion indicator lights 2 Press the right arrow until TANK SELECT X is dis played in the message center 3 Press up down arrow to toggle setting between 1 and 2 4 Press Enter to save the setting 5 Press the Mode key to return to Run mode or right left to go to next previous parameter Steri Cult Incubator ______________________________________________________________________Configuration 4 3 n Disabli...
Page 20: ...Steri Cult Incubator _______________________________________________________________________Configuration 4 4 ...
Page 21: ...Steri Cult Incubator _______________________________________________________________________Configuration 4 5 ...
Page 22: ...Steri Cult Incubator _______________________________________________________________________Configuration 4 6 ...
Page 23: ...min No CO2 CO2 High Tracking Alarm CO2 IS HIGH 0 min 15 min Prog CO2 CO2 Low Tracking Alarm CO2 IS LOW 15 min 15 min Prog No water flow through the water valve NO WATER FLOW 5 min 15 min No TEMP TEMP Low Tracking Alarm TEMP IS LOW 15 min 15 min Prog RH RH Low Limit Alarm RH IS LOW 30 min 15 min Prog RH RH High Limit Alarm RH IS HIGH 0 min 15 min Prog RH System Fault CHECK RH SYSTEM 60 min 15 min N...
Page 24: ...he other tank However an audible alarm and warning light activate indicating the low tank condition Pressing Silence clears the alarm condition and does not ringback The warning light on the display remains activat ed until the tank is replaced and both tanks are normal If the tank not in use goes low the warning light is activat ed If both banks go low the audible alarm and warning light are acti...
Page 25: ...t filter assembly If it is older than six months replace with new 2 Humidifier tubing pinched or 2 Check to ensure tubing is not pinched or disconnected disconnected 3 Humidifier has heavy build up 3 Clean the humidifier see Section 6 3 4 RH sensor not reinstalled correctly 4 Ensure RH sensor bottoms out in hole Condensation on cabinet face 1 Removable gasket not reinstalled 1 Ensure there are not...
Page 26: ...aved 3 Wash the inner door gasket with disinfectant This gas ket may be removed to be cleaned or replaced 4 Wash the cabinet interior with disinfectant starting at the top and working down Refer to the disinfectant label for instruction on the length of time needed before rinsing Wash the inner door s both inside and out The cabinet and door must be rinsed with sterile water until the disinfectant...
Page 27: ...ith sterile water and spray with 70 alcohol Steri Cult Incubator ______________________________________________________________________Maintenance 10 Also behind the access door is the humidifier See Figure 7 4 Lift the latches on both sides of this small unit 11 Separate the top and bottom sec tions Carefully clean the inside of both sections and spray with 70 alcohol Do not touch the metal disk ...
Page 28: ...Model 3307 3308 ______________________________________________________________________Maintenance 7 3 Figure 7 5 orientation for 3310 3311 orientation for 3307 3308 ...
Page 29: ...ll be fixed at setpoint A HEPA filter replacement kit see the spare parts list should be on hand prior to initiating the Sterilization Cycle Information About the Cycle Pre cleaning may be required In order to avoid odors stains on the interior baked on material etc wipe off all visible signs of spills Odor may occur during the Sterilization Cycle and is considered normal The Sterilization Cycle i...
Page 30: ...E resumes if the chamber temperature was less than 90 C when power was HEAT PHASE interrupted HEAT PHASE resumes if the chamber temperature was greater than 90 C when power was interrupted and the temperature dropped less than 1 C CANCELED COOL PHASE starts if the chamber temp drop is more than 1 C Power interruption during the HEAT PHASE begins if the chamber temperature has not dropped below 139...
Page 31: ... shows the bottle removed for clarity Locate the RH and CO2 sensors Figure 8 2 6 Pull the sensors out ward carefully Allow them to hang See Step 12 for the sensor disinfecting procedure 7 Secured in the same area are sensor hole plugs Install a plug into each sensor location Close the access door Steri Cult Incubator _______________________________________________________________________Sterilizat...
Page 32: ...ach the hole limit RH and CO2 sensors must be reinstalled correctly for systems to properly sense chamber conditions If not RH and CO2 levels may be uncontrolled 14 Open the chamber and replace the present HEPA with a new one For instructions to install see Section 1 5f 15 Install the new access port filter assembly into the upper left corner of the back of the chamber Section 1 5e Press ENTER 16 ...
Page 33: ...ified service technician The following is a condensed list of preventive maintenance requirements See the specified section of the instruction manual for further details We have qualified service technicians using NIST traceable instruments available in many areas For more information on Preventive Maintenance or Extended Warranties please contact us at the number listed below Cleaning and calibra...
Page 34: ...ly 3 to 6 Months Yearly Check CO2 tank levels Inspect door latch hinges and door gasket seal 2 Verify and document CO2 humidity and temperature calibration as applicable 6 Thoroughly clean the interior of the incubator 1 5e f Replace HEPA and access port filters or as needed 11 3 Replace CO2 and RH air filters 5 3 Clean humidity system 7 Perform Sterilization Cycle as needed Qualified service tech...
Page 35: ...bator display is 1 unit There is no cal ibration from the incubator Calibration to the incubator display must be at the instrument con nected to the output board Section 9 Factory Options 9 1 Connections to External Equipment a Connecting the Remote Alarm Contacts A set of relay contacts is provided to monitor alarms through an RJ11 telephone style connector on the back of the cabinet Refer to Fig...
Page 36: ...uipped with two stage pressure regulators with gauges The high pressure gauge at the tank should have a 0 2000 psig range and the low pressure gauge should have a 0 30 psig range The gas supply to the incubator must be maintained at 15 psig 1 034 bar Gas pres sures below 15 psig will cause nuisance alarms to occur on incubators equipped with the built in Gas Guard a Connecting the CO2 Gas Supplies...
Page 37: ...k Low indicator on the control panel will stay light ed until the condition is resolved The unit will operate normally Both the audible and visual alarms described above do not ring back after the Silence key is pressed If the Gas Guard system does not detect an adequate gas supply at CO2 Inlet 1 or Inlet 2 a visual and audible alarm will again occur on the control panel The visual alarm in the Me...
Page 38: ... system 95 RH at 37 C Setpoint Digital touch pad 1 resolution Display Digital LED 1 resolution CO2 Control 0 1 5 0 Sensor Infrared Setpoint Digital touch pad 0 1 C resolution Range 0 to 20 factory default 0 disabled Controller Microprocessor Display Digital LED 0 1 increments Shelves Standard 5 Maximum 22 Construction Stainless steel perforated Clearance Adjustable on 1 160 centers Dimensions 3307...
Page 39: ...l 3307 3308 400 lbs 181 4kg Model 3310 3311 480 lbs 217 7kg Certifications Refer to the Declaration of Conformity at the end of this manual Safety Specifications Indoor Use Only Altitude Up to 2 000 meters Temperature 5 C to 40 C Humidity Maximum 80 RH for temperatures up to 31 C decreasing linearly to 50 RH at 40 C Mains Supply Fluctuations Mains supply voltage fluctuations not to exceed 10 of th...
Page 40: ...uard option 250121 Solenoid Gas valve 12VDC Gas Guard option Steri Cult Incubator ________________________________________________________________________Servicing 11 1 Parts List continued Part Number Description 113008 Leveling foot 770001 CO2 filter 1900161 VOC HEPA filter kit includes 760208 1900185 1900185 Access port filter with stopper 760213 RH air filter 760207 Chamber main HEPA only 2500...
Page 41: ...partment 11 3 Replacing the CO2 and RH Air Filters The following procedure requires accessing the electronics area Qualified personnel only should perform this procedure Wearing a electrostatic discharge wrist band or other grounding device is recommended The CO2 and RH air filters are recommended to be replaced annually Follow the steps below to replace the filters 1 Turn off the incubator s powe...
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Page 53: ...ither the equipment or to the component part beyond the original warranty period The Technical Services Department must give prior approval for return of any components or equipment At Thermo s option all non conforming parts must be returned to Thermo Electron Corporation postage paid and replacement parts are shipped FOB destination THIS WARRANTY IS EXCLUSIVE AND IN LIEU OF ALL OTHER WARRANTIES ...
Page 54: ... warranty to either the equipment or to the component part beyond the original warranty period The Technical Services Department must give prior approval for return of any components or equipment At Thermo s option all non conforming parts must be returned to Thermo Electron Corporation postage paid and replacement parts are shipped FOB destination THIS WARRANTY IS EXCLUSIVE AND IN LIEU OF ALL OTH...
Page 55: ...Steri Cult Incubator ___________________________________________________________________________Appendix A Appendix A ...
Page 56: ... Push locking sleeve forward on connector until it is flush with the front of the connector 4 If sleeve is difficult to slide on a new connector exercise it a few times Do not use tools With TURCK s patented Locking Sleeve pulled back any picofast sensor slides on without any difficulty After sensor is connected push Locking Sleeve for ward to create a watertight connection It s a snap 1 Do not tw...
Page 57: ...Thermo Electron Corporation Controlled Environment Equipment Millcreek Road P O Box 649 Marietta Ohio 45750 U S A _________________________ Telephone 740 373 4763 Telefax 740 373 4189 ...
