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GENESYS™ 10 Series

Spectrophotometers

Operator’s Manual

Summary of Contents for GENESYS 10

Page 1: ...GENESYS 10 Series Spectrophotometers Operator s Manual ...

Page 3: ...el posterior del espectrofotómetro Panneau arrière du spectrophotomètre Rückwand des Spektrophotometer Pannello posteriore dello spettrofotometro Key Leyenda Légende Legende Legenda c A C power connector Conector C A Connecteur courant A C Stromanschluss Connettore alimentazione CA d RS232C port Salida RS232C Port RS232C RS232C Port Porta RS232C e Analog BNC connector Conector analogo Connecteur a...

Page 4: ...e de direction du papier Symbol für die Papierrichtung Indicazione direzione carta e Paper entry slot Ranura de entrada del papel Fente d entrée du papier Papiereinzugsschlitz Fessura entrata carta f Finger tab Lengüeta Languette Lasche Braccetto Key Leyenda Légende Legende Legenda c A C power connector Conector C A Connecteur courant A C Stromanschluss Connettore alimentazione CA d Parallel port ...

Page 5: ...espectrofotómetro GENESYS 10 Clavier du spectrophotomètre GENESYS 10 Tastatur des GENESYS 10 Spektrophotometer La tastiera sullo spettrofotometro GENESYS 10 Key Leyenda Légende Legende Legenda c Cell position keys for 6 Position Cell Holder Teclas de posicion de celda para portaceldas de 6 Posiciones Touches de position des cuves pour porte cuves à 6 emplacements Kuevetten Positionstasten für den ...

Page 6: ...izzato g Aligning the lamp assembly Alineando el ensamble de la lámpara Alignement du bloc lampe Justieren der Lampe Allineamento del portalampada Figure 6 Installing the printer 335988 Instalando la impresora 335988 Installation de l imprimante 335988 Installieren des Druckers 335988 Installazione della stampante 335988 Key Leyenda Légende Legende Legenda c Captive screw on lamp door Tornillo en ...

Page 7: ... Abdeckung Rimozione del coperchio del compartimento fusibili Figure 9 Removing the fuse holder Removiendo el porta fusible Enlèvement du porte fusibles Entfernen des Sicherungshalters Rimozione del portafusibile Figure 10 Removing and replacing the fuses Removiendo y reemplazando los fusibles Enlèvement et remplacement des fusibles Tauschen der Sicherungen Rimozione e sostituzione dei fusibili ...

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Page 11: ...umstances shall Thermo Spectronic be obligated to notify purchasers of any future changes in either this or any other instructions or specifications relating to Thermo Spectronic products nor shall Thermo Spectronic be liable in any way for its failure to notify purchasers of such changes GENERAL SAFETY NOTES USED IN THIS MANUAL This symbol alerts you to important information about using the instr...

Page 12: ...ents may be warranted by their manufacturer Thermo Spectronic is not responsible under this warranty for loss in operating performance due to environmental conditions THIS WARRANTY IS IN LIEU OF ALL WARRANTIES EXPRESSED IMPLIED OR STATUTORY INCLUDING BUT NOT LIMITED TO WARRANTIES OF FITNESS FOR A PARTICULAR PURPOSE OR MERCHANTABILITY OR OTHERWISE and states Thermo Spectronic s entire and exclusive...

Page 13: ...ity section you can lock test setups test parameters so that they may not be altered The password also allows you to remove the security so that you may edit the test parameters Please refer to the appropriate section in this Operator s Manual for more information on locking a test PASSWORD 4 3 6 3 7 9 7 Thermo Spectronic 820 Linden Avenue Rochester NY 14625 585 248 4000 or 800 654 9955 ...

Page 14: ...iv GENESYS 10 Operator s Manual ...

Page 15: ...ng the cell correction program 2 2 Taking measurements 2 4 Scanning tests 2 5 Basic Absorbance T measurements 2 5 Setting the wavelength 2 6 Measuring a blank 2 6 Measuring unknowns 2 6 Basic Concentration measurements 2 6 Setting the wavelength mode 2 6 Measuring a blank 2 6 Measuring a standard 2 6 Entering a factor 2 7 Measuring unknowns 2 7 Advanced A T C Absorbance Transmittance measurements ...

Page 16: ...aseline scan 2 20 Scanning an unknown 2 21 Viewing manipulating scan data 2 21 3 Point Net 2 23 Recalling a test 2 23 Setting up test parameters 2 24 Taking measurements 2 24 Multiple Wavelengths 2 24 Recalling a test 2 25 Setting up test parameters 2 25 Taking measurements 2 25 CHAPTER 3 Using the Performance Validation Program Overview 3 1 Accessing the Performance Validation tests 3 1 Troublesh...

Page 17: ... in the internal printer 4 3 External printers 4 3 External computers 4 3 CHAPTER 5 Performing Maintenance Procedures Routine care 5 1 Cleaning 5 1 Cleaning and maintenance of cells 5 1 Cleaning the windows of the sample compartment 5 2 Changing the fuse 5 2 Replacing the tungsten lamp 5 2 Replacement parts 5 3 Appendix A Specifications Appendix B Parameters Appendix C Calculations Index Table of ...

Page 18: ...Table of Contents viii GENESYS 10 Operator s Manual ...

Page 19: ...ve any obstructions or materials that could hinder the flow of air under behind and around the instrument 4 Connect the female end of the power cord into the connector labeled A C power on the back panel of the instrument see Figure 2 5 Plug the other end of the power cord into a grounded outlet with the appropriate voltage 6 Ensure that the sample compartment does not contain any samples and that...

Page 20: ...ight Set Date and press ENTER Press Set Day type the date then press ENTER Press Set Month highlight the correct month then press ENTER Press Set Year type the year then press ENTER When the date is correct press ESC to save the settings and return to the Utility screen To select the time format You can set up the spectrophotometer to display the time in either am pm format or in 24 hour format To...

Page 21: ...in Figure 3 When installed correctly they will fit flush with the top of the instrument Cut the paper so the edge is even Note Arrows on the paper roll holders indicate the direction of the paper feed see Figure 3 Feed the paper straight into the paper entry slot The printer grabs the end of the paper and pulls it in In Basic Absorbance Transmittance T when the paper stops press ENTER to continue ...

Page 22: ...n in the light beam A fiducial mark on the test tube helps you orient the test tubes consistently and correctly When using small aperture cells Always use masked cells Use the same cell or cuvette for your blank and your samples Correcting for cell variability Due to variability in cell manufacture each cell may have slightly different absorbance and reflection characteristics Although it would be...

Page 23: ... to select the characters for the filename from a character list 1 After setting up the values for the test parameters press Save Test The Create Test Name screen appears You can use this screen to Delete the name of a test Delete a character in the name of a test Add a character to the name of a test Accept the name of a test 2 Use the arrow keys to highlight the first character you want to use f...

Page 24: ...l the characters for the name 4 Press Accept Name to accept the name and return to the previous screen The name of the new custom unit appears on the list of basic units Using the SmartStart feature GENESYS 10 UVscanning model only The SmartStart feature enables you to select the test methods you use most frequently and have them appear when you start up your instrument If your laboratory runs onl...

Page 25: ...e Cell Correction program is not active in the Main Basic Absorbance T Basic Concentration screen Note The Cell Correction feature is active only when the 6 Position Sample Holder is set to either Auto 6 or Auto 3 The feature is not active when the cell holder is set to 1 Cell Platform or Manual 6 nor when the Single Cell Holder is installed Every test setup screen provides access to the cell corr...

Page 26: ...crete nms mode first specify the wavelengths using the procedures which follow and then the Cell Correction program The Cell Correction program will measure the other cells against the blank and will record store and date the measurements From these measurements the Cell Correction program establishes the required correction factors which then are automatically applied during all subsequent tests ...

Page 27: ... do not function when you select Single Cell Platform even if you have a 6 Position Cell Holder installed in the sample compartment Manual 6 You can take up to six measurements without changing the samples in the cell holder using the cell position buttons on the keypad to advance the cell holder to the appropriate position for the next measurement You place the blank in the blank position and you...

Page 28: ...he cell holder If your instrument has a 6 Position Cell Holder be sure to place the blank in the B position 2 If you want to enter an absorbance or transmittance value for the blank press a number key and enter the concentration in the Entry field 3 Press Measure Blank to measure the blank When the instrument is finished measuring the absorbance of the blank the message disappears Measuring unknow...

Page 29: ...ation for the blank press a number key and enter the concentration in the Entry field 3 Press Measure Blank to measure the blank When the instrument is finished measuring the absorbance of the blank the message disappears Measuring a standard 1 If necessary press Change Mode to switch to the Concentration with Standard mode 2 If your instrument is equipped with the 6 Position Cell Holder place the...

Page 30: ... appears on the display If your instrument is equipped with the Single Cell Holder Platform remove the blank and place the unknown in the cell holder The measurement appears on the display Advanced A T C Absorbance Transmittance measurements When you use the Advanced A T C program for absorbance or transmittance measurements you can perform these tasks Select the measurement mode you want to use A...

Page 31: ...The Advanced A T C measurement screen appears prompting you to place your samples in the cell holder 2 Place the blank and unknowns in the cell holder If your instrument is equipped with a 6 Position Cell Holder be sure to place the blank in the B position You can place up to five samples in the cell holder 3 Press Measure Blank to measure the blank If your instrument is equipped with a 6 Position...

Page 32: ... a standard automatically using Auto 6 or Auto 3 1 With the Advanced A T C screen displayed and the Measurement Mode set to Conc Std press Run Standard The Measure Standard screen appears 2 Use the number keys to enter the concentration value of the standard and press ENTER 3 Press Measure Standard 4 Place the blank and standard in the correct cell positions 5 Press ENTER to measure the blank and ...

Page 33: ...ly using Manual 6 or Single Cell Platform 1 With the Advanced A T C screen displayed and the Measurement Mode set to Conc Std or Conc Factor press Run Test The Advanced A T C measurement screen appears 2 Place the blank and unknown in the correct cell position If your instrument is equipped with a 6 Position Cell Holder you can place up to five samples in the cell holder 3 Press Measure Blank to m...

Page 34: ... When you ve entered all the concentration values the Standards screen appears 4 Press Measure Standards to measure the blank and standards The instrument automatically measures the blank first then measures the standards When the instrument has measured all the standards the Standards screen appears showing the absorbance of each standard along with the slope intercept and correlation coefficient...

Page 35: ...ch between tabular and graphical displays press View Graph Tabular Measuring unknowns manually using Manual 6 or Single Cell Platform 1 With the Standards screen displayed press Run Test The Samples screen appears 2 Place the blank and unknowns in the correct cell positions 3 Press Measure Blank to measure the blank If your instrument is equipped with a 6 Position Cell Holder it automatically move...

Page 36: ...he standard To clear measurements 1 With the standard curve displayed on your screen press Edit Standards 2 Use the arrow keys to highlight Clear Measurements and press ENTER All the absorbance measurements will be removed from the screen To reset standards 1 With the standard curve displayed on your screen press Reset Standards and press ENTER 2 Use the arrow keys to highlight Reset Standards and...

Page 37: ...ameter you want to set 2 When the parameters are set you can press Save Test to save the test or Run Test to measure a blank or unknowns Measuring unknowns Measuring unknowns automatically using Auto 6 or Auto 3 1 Place the blank and unknowns in the correct cell positions 2 Press ENTER to start the measurement The instrument automatically measures the blank first then measures the unknowns and dis...

Page 38: ... this screen you can Set up test parameters Set up Cell Correction program GENESYS 10 UVscanning model only Save a test View the list of stored tests Measure a blank Measure unknowns Setting up test parameters 1 With the Absorbance Difference screen displayed use the arrow keys to highlight the name of the parameter you want to set 2 When the parameters are set you can press Save Test to save the ...

Page 39: ... on the type of display you choose Note The Kinetics program allows you to measure only one unknown at a time Note The Kinetics program allows you to collect up to 400 data points per run When you set up your test parameters be sure to select the interval time and total run time accordingly To get started press the TEST key on the keypad When the Test Types screen appears highlight Kinetics and pr...

Page 40: ...aphical form or in tabular form When your results are displayed on the screen you can modify the range start and stop time and the instrument automatically recalculates the reaction rate When you are working with graphical kinetics results you need to press Edit Graph before you can rescale and recalculate You can modify the scale of your kinetics data plot in two ways automatically or manually Wh...

Page 41: ...ult Rescaling recalculating tabular kinetics results When you are working with tabular kinetics results you need to press Edit Data before you can rescale and recalculate After collecting your kinetics data you can use all the data for the rate calculation or you can select specific start and end times Whenever you modify the start and end times or select all the data the instrument automatically ...

Page 42: ...anning and press ENTER Recalling a test 1 With the Survey Scan Scanning screen displayed press Stored Tests A list of stored tests appears 2 Use the arrow keys to highlight the name of the test you want to recall and press ENTER The parameters for the selected test appear on the screen From this screen you can Set up test parameters Set up Cell Correction program GENESYS 10 UVscanning model only S...

Page 43: ...u are working with graphical scan data you need to press Edit Graph before you can perform other functions on the scan data When the edit graph screen appears you can Rescale the graph Perform mathematical operations on the graph Rescaling graphical scan data You can modify the scale of your scan data plot in two ways automatically or manually When you select Auto Scale the instrument automaticall...

Page 44: ...ss the appropriate function key Min Y Max Y Min X Max X to accept it The instrument redraws the plot using the minimum and maximum values you have entered To use the Auto Scale function 1 With your scan data displayed on the edit scale screen press Auto Scale The instrument automatically adjusts the minimum and maximum values for the X and Y axes so all the data appears on the plot Performing calc...

Page 45: ...The instrument calculates the area under the curve for the selected wavelengths 4 Continue selecting the other wavelengths by pressing Next Cursor to activate the next cursor line Select the wavelength by positioning the cursor with the Cursor and Cursor keys 5 Press Set Options to access the set options window 6 Use the up and down arrows to highlight Factor Enter the desired factor and press ENT...

Page 46: ...started press the TEST key on the keypad When the Test Types screen appears highlight 3 Point Net and press ENTER Recalling a test 1 With the 3 Point Net screen displayed press Stored Tests A list of stored tests appears 2 Use the arrow keys to highlight the name of the test you want to recall and press ENTER The parameters for the selected test appear on the screen From this screen you can Set up...

Page 47: ... cell position 4 Press Measure Sample to measure the unknowns The sample measurement appears on the screen If your instrument is equipped with a 6 Position Cell Holder press the cell position buttons on the keypad to reposition the cell holder and measure the rest of the unknowns manually Multiple Wavelengths When you use the Multiple Wavelengths program you can perform these tasks Recall a test O...

Page 48: ...velength and factor pair 3 Press Add nm 4 Enter the values for the wavelength and factor and press ENTER 5 When the values are correct press Add nm 6 Continue until you have entered all the wavelengths and factors Deleting wavelengths factors 1 With the Multiple Wavelengths screen displayed use the arrow keys to highlight Set nms The wavelength screen appears listing the wavelengths and factors sp...

Page 49: ...ll Holder it automatically moves to the B position to measure the blank When it completes the measurement it returns to its previous cell position 4 With the list of wavelengths and factors displayed press Measure Samples to measure the unknowns The instrument measures the absorbance at each wavelength and displays it on the screen If you have set the measurement mode to Concentration Factor the i...

Page 50: ...Using the Instrument 2 28 GENESYS 10 Operator s Manual ...

Page 51: ...r you have tried all the recommendations in the list follow the troubleshooting list for the test being run included with the description of each test Make sure you are following the instructions for the test properly Make sure filters and standards are clean Make sure the sample compartment door is closed while the test is running Make sure the sample compartment is clear of any obstructions Make...

Page 52: ...nge them to the values on the certificate of calibration included with your standards Note Only the middle wavelength of the SPECTRONIC Standards wavelength standard is certified The wavelength accuracy test passes or fails based on this wavelength alone The other two wavelengths may be used for long term repeatability testing The initial target values may be determined by running the wavelength a...

Page 53: ... and compares the results with specified tolerances The absorbances and tolerances are preset but you should change them to the values on the report of calibration included with your standards Note You can display the tolerances for this test in either absorbance or transmittance 1 With the Performance Validation screen displayed use the arrow keys to highlight Photometric Accuracy 2 Press ENTER T...

Page 54: ... consistently Make sure you follow the guidelines provided with the standard reference materials Contact technical support for more troubleshooting advice Noise Measurement This test measures the amount of noise peak to peak at specified wavelengths UV Vis instruments measures noise at 230 and 340nm Vis instruments measures noise at 500nm All parameters are preset and cannot be changed When runnin...

Page 55: ...u may use other filters but they must have 0 1 T at the wavelength of interest 1 With the Performance Validation screen displayed use the arrow keys to highlight Stray Light 2 Press ENTER The Stray Light screen appears Running the test 1 With the Stray Light screen displayed make sure that the wavelengths and tolerances are set correctly 2 Press Start Test to run the test The results of the test a...

Page 56: ... Performance Validation screen displayed use the arrow keys to highlight RS232C Test 3 Press ENTER The RS232C Test screen appears 4 Press Start Test to run the test When the test is complete the message RS232 OK or RS232 Failed appears on the screen While the test is running you can press Stop Test to stop it If the test fails follow these guidelines Make sure that the test plug is installed corre...

Page 57: ...Holder holds three or 335916 Single Cell Holder Platform holds one 335079 Thermal Block Controls the temperature of one 10mm pathlength square cuvette within the range of 20 to 100ºC fits on 335927 6 Position Cell Holder holds three or 335916 Single Cell Holder Platform holds one requires external water bath and 335920 Accessory Door for routing circulating water 336028 Single Cell Holder Accommod...

Page 58: ...s not aligned correctly you will not be able to tighten the screws Connecting Using Accessories 4 2 GENESYS 10 Operator s Manual Catalog Description 335982 Peristaltic Pump Includes set of Norprene pump tubing used with 335171 Optical Glass flowcell or 335172 Quartz flowcell and 335920 Accessory Door Kit 115V 50 60Hz 335982 02 Same as 335982 except with Europlug 230 VAC 335982 04 Same as 335982 ex...

Page 59: ...ure 6 and faces away from the printer and towards the plastic door 9 Use the clip on the hinge to secure the wires 10 Install the printer door by sliding it back onto the hinge 3 Figure 6 11 Close the lamp door 12 Tighten the captive screw 1 Figure 6 on the printer door to hold it securely in place 13 Load the paper into the printer see instructions in Setting up the instrument Loading the paper i...

Page 60: ...Connecting Using Accessories 4 4 GENESYS 10 Operator s Manual ...

Page 61: ...e to the instrument If concentrated acids or bases or any hydrocarbon materials are spilled on the instrument be sure to clean up the affected area immediately Use water alcohol or other common laboratory cleaning agents to clean the keyboard It is recommended that you clean spills off the keyboard as soon as they occur Cleaning Cleaning and maintenance of cells Cleaning of cells both inside and o...

Page 62: ...t Figure 2 120VAC 2 5A Slo Blo 240VAC 1 25A Slo Blo 2 required See Replacement parts on page 5 3 for a list of replacement part numbers WARNING The instrument fuse must be replaced with the same type and rating fuse WARNING If the fuse fails repeatedly it may indicate a serious problem with the instrument Contact your service representative as soon as possible 1 Turn off and unplug the instrument ...

Page 63: ...foot roll 335923 Tungsten Lamp Assembly For all GENESYS 10 Vis spectrophotometers Replacement parts 1 Turn off and unplug the instrument 2 Make sure that the lamp has had time at least 10 minutes to cool off 3 Loosen the captive screw 1 Figure 7 on the lamp door by rotating it counterclockwise about turn 4 Raise the lamp door 5 Unscrew the thumbscrew 2 Figure 7 holding the lamp assembly 3 Figure 8...

Page 64: ...Performing Maintenance Procedures 5 4 GENESYS 10 Operator s Manual ...

Page 65: ...nm Standard Interface Bi directional RS232C Bi directional RS232C parallel output in PCL format Cell Holder 1 position OR automatic 6 position cell holder Keyboard Membrane keypad Software Absorbance Transmittance Concentration As G10 UV but with full Standard Curve Absorbance range scanning Ratio Absorbance Difference Kinetics Scanning speed 200 Survey Scan 3 Point Net Multiwavelength 1000nm min ...

Page 66: ...80 VA max Operating environment The instrument meets the specifications on the previous page under the following conditions after a 30 minute warm up period Ambient temperature 5 to 40 C 23 F to 104 F Relative humidity 20 80 RH Storage environment 20 C to 70 C 24 8 F to 158 F Relative humidity not to exceed 0 040 pounds moisture per pound of dry air Allow instrument to adjust to room temperature f...

Page 67: ...first measurement allows for sample equilibration Factor Abs x Factor 1 Concentration Abs min x Factor 2 Result Factor 1 Abs WL1 x Factor Result Factor 2 Abs WL2 x Factor Result Factor 3 31 Abs WL3 31 x Factor Result ID Numeric Identifier autoincrements during test until reset or test is exited Intercept Where line crosses the y axis abs where conc 0 Interval Time Time between repeated readings La...

Page 68: ...min for a scan Setup Correction Initiates the procedure to collect the data necessary to correct for cuvette variances Slope Abs Concentration SRE Tolerance Stray radiant energy acceptable Standby Time since last keystroke or instrument activity powers down unit to minimum energy required Start Wavelength Beginning wavelength for a scan Statistics Turns stats on or off if ON calculates average and...

Page 69: ...ard N number of standards A A c where A absorbance c concentration A c is defined by an equation of the form A c a4c4 a3c3 a2c2 a1c a0 where a0 Y axis intercept a1 a4 coefficients The coefficients are computed using the least squares method A a1 c where A absorbance c concentration a1 slope The slope is calculated as a1 SXY SXX This model requires Slope is not equal to zero At least one standard d...

Page 70: ...n and the message Curve cannot be used to determine unknown concentrations it may produce ambiguous results will appear when the curve is viewed where n degree of polynomial Note The calculation for the correlation coefficient only applies to first order linear regression curves first degree polynomials Absλ1 or Absλ1 Absref Absλ2 Absλ2 Absref Result Absλ1 factor 1 Absλ2 factor 2 or Absλ1 Absλref ...

Page 71: ...GENESYS 10 Operator s Manual C 3 Calculation Calculation s Graphs 3 Point Net 3 Point Net Absorbance sample curve Baseline corrected absorbance A2 A3 A1 A3 λ3 λ2 λ3 λ1 Appendix C Calculations ...

Page 72: ...Appendix C Calculations C 4 GENESYS 10 Operator s Manual ...

Page 73: ... 1 Calibration 1 1 Cell Correction program 1 4 2 3 2 4 2 5 2 8 2 9 2 12 2 15 2 16 2 17 2 18 2 20 2 24 2 25 2 26 Cell holders cell holder accessories 2 4 4 1 4 2 5 3 Cleaning 5 1 5 2 Concentration measurements 2 6 2 10 Concentration units 2 1 2 2 2 6 2 7 Custom units 2 1 2 2 D Date and time setting 1 2 E External computers 4 3 F Factor entering 2 7 2 8 2 10 2 11 2 26 B 1 Fuses changing 5 2 G Glassw...

Page 74: ... 2 25 2 26 A 1 Screen contrast setting 1 3 Single Cell Platform 2 5 Single Position Cell Holder installing 5 Slope 2 12 2 13 B 2 C 1 SmartStart 2 2 Software password iii Specifications A 1 SPECTRONIC Standards 3 2 3 3 3 5 4 2 5 3 A 1 Standard Curve 2 11 editing 2 14 measuring the standards for 2 12 Standard measuring 2 7 Standby settings selecting 1 2 Start Wavelength B 2 Stop Wavelength B 2 Stray...

Page 75: ...W Warranty ii Wavelength accuracy 3 1 internal 3 1 standards 3 2 Z Z dimensions 1 2 Index GENESYS 10 Operator s Manual I 3 ...

Page 76: ...Index I 4 GENESYS 10 Operator s Manual ...

Page 77: ...8HY UK Telephone Int 44 0 1223 446655 Fax Int 44 0 1223 446644 E Mail info thermospectronic co uk Web Site www thermospectronic com THERMO SPECTRONIC Asia Pacific Middle East Africa India and Latin America Contact Rochester New York USA SPECTRONIC is a registered trademark and GENESYS is a trademark of Thermo Spectronic Thermo Spectronic is an ISO 9001 Systems Certified Company Themo Electron Spec...

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