Thermo Scientific VF-F20-A, Operating Manual

Page: 171 / 240

7
•
Maintenance and Service
Integral Fraction Collector FT (VF-F20-A) Operating Manual Page 171
13. In Chromeleon, make sure to check the installed flush buffer loop
compatible with the installed needle capillary. Both the needle
capillary and flush buffer loop shall match with the application of
flow rate range.
Flow rate [mL/min] Needle Capillary ID x L
[mm] Flush Buffer Loop ID x L
[mm]
0 - 5.0 0.18 × 415 0.5 × 255 (V = 50 µL)
5.0 - 10.0 0.25 × 415 0.5 × 510 (V = 100 µL)
NOTICE The needle capillary must match with installed flush buffer loop
as shown in above table in order to achieve good fraction collection
performance with low carry over and high recovery when flush function
is enabled. The fluidic volume of flush buffer loop shall be around
double volume of matched needle capillary.
14. Flush the flush pump solvent lines to ensure they are filled with
fresh flush solvent, see Performing A Flush Purge Cycle (

Page 171).
7.8.3 Performing A Flush Purge Cycle
When
•
Routine and preventive maintenance for the flush purge system
•
Replacing the flush solvent
•
Replacing flush purge lines
Preparations
•
Observe the Flush Solvent Guidelines . See Flush Solvent Guidelines
(

page 164) .
Follow the step
In Chromeleon, select Purge Flush Pump on the ePanel FC tab to fill the
flush pump with the fresh flush solvent. During purging, the flush pump
and the flush buffer loop is washed continuously until all flush purge
lines filled with fresh flush solvent.