Thermo Scientific VF-F20-A, Operating Manual

Page: 118 / 240

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Operation
Page 118 Integral Fraction Collector FT (VF-F20-A) Operating Manual
Parameters Description
Delay volume The Fraction Delay Volume is the fluidic
volume travelled by a peak after triggering
the set start threshold conditions between a
detector and the separation point of the
downstream fraction collection valve.
The volume can be logged automatically if the
Vanquish Fraction Collector Delay Volume
Determination
operational qualification is
performed. Before running the OQ test, it is
recommended to perform first an Instrument
Installation Qualification to ensure that the
standard HPLC system is basically ready to
operate in its intended use.
For details, see Delay Volume Determination
(DVD) (

page 120).
The Delay Volume can be changed by
specifying delay Capillary inner diameter (ID)
and length (L).
Delay capillary
IDxL Select either Custom or predefined Delay
Capillary IDxL . If the predefined Delay
Capillary IDxL is selected , the Delay Volume
is automatically updated.
Delay capillary
ID
Delay capillary L
If Custom as Delay Tubing Capillary IDxL is
selected, then need to manually input Delay
Capillary ID and Delay Capillary L . The Delay
Volume is calculated and displayed
accordingly.
Fraction collection options
Off (do not
collect at all) Select this option to disable fraction
collection.
Collect by peak When this option is selected, fraction
collection starts depending on the specified
detection channels and peak detection
settings.
Collect by time Fraction collection is independent of peak
detection.
When this option is selected, use the
Collection period input field to specify the
time in [s] after which a new tube will be
used.
Tube wrapping Select this check box to return to the first
tube after the last tube is filled. If this check
box is cleared, the Chromeleon Queue will be
aborted when the last tube is reached.