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6

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Operation

Page 120

Integral Fraction Collector FT (VF-F20-A) Operating Manual

6.7

Delay Volume Determination (DVD)

6.7.1

Delay Volume and Time

The Delay Volume is the fluidic volume between a detector and the
separation point of the downstream fraction collection valve. It is
essential to determine the volume and set it in the system before
starting fraction collection operation.

The product provides the feature Automated Delay Volume
Determination (DVD), with which the delay volume can be determined
conveniently and set in the system automatically.

The delay time can be automatically calculated and set in the system
based on the delay volume and specified method configuration, e.g.
pump flow rate.

The correct delay time ensures optimal fraction collection of the target
peak with high recovery and low carry over.

Figure 43: Fraction collection valve located at waste position when
running delay volume determination

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Summary of Contents for VF-F20-A

Page 1: ... 4827 6001 EN 1 0 Vanquish Integral Fraction Collector FT VF F20 A Operating Manual 4827 0601 EN Revision 1 0 August 2022 thermoscientific ...

Page 2: ... of Thermo Fisher Scientific Inc This manual is provided as is The contents of this manual are subject to being changed without notice in future revisions Thermo Fisher Scientific Inc makes no representations that this document is complete accurate or error free Thermo Fisher Scientific Inc assumes no responsibility and will not be liable for any errors omissions damage or loss that might result f...

Page 3: ...on or sales support for HPLC products contact your local Thermo Fisher Scientific sales organization For contact information go to Contact Us on http www thermoscientific com Technical Assistance For technical support for HPLC products contact your local Thermo Fisher Scientific support organization For contact information go to Contact Us on http www thermoscientific com ...

Page 4: ...Contacting Us Page 4 Integral Fraction Collector FT VF F20 A Operating Manual ...

Page 5: ...al 19 2 1 2 Observing this Manual 19 2 1 3 Safety Symbols on the Device 20 2 1 4 Applicable Symbols on the Device 20 2 1 5 Rating Plate 20 2 2 Intended Use 21 2 3 Safety Precautions 22 2 3 1 General Safety Information 22 2 3 2 Qualification of the Personnel 23 2 3 3 Personal Protective Equipment 23 2 3 4 Electrical Safety Precautions 24 2 3 5 General Residual Hazards 25 2 3 6 In Case of Emergency ...

Page 6: ...ion 56 5 2 Installing the Device 57 5 3 Site Requirements 59 5 3 1 Power Considerations 59 5 3 2 Power Cord 60 5 3 3 Condensation 61 5 4 Accessing the Interior Components 62 5 5 Setting Up the Hardware 63 5 5 1 System Arrangement 63 5 5 2 Connecting the Device 65 5 6 Setting Up the Flow Connections 68 5 6 1 General Information and Guidelines 68 5 6 2 Guiding Capillaries and Tubing Through the Syst...

Page 7: ...ctor Compartment 107 6 5 2 Loading the Carousel 107 6 5 3 Rack Type Settings 111 6 5 4 Needle Height Setting 111 6 6 Important Operating Parameters 113 6 7 Delay Volume Determination DVD 120 6 7 1 Delay Volume and Time 120 6 7 2 When to Perform DVD Function 121 6 7 3 How to Perform DVD Function 122 6 7 4 Delay Volume Capillary Drop Down Menu 124 6 7 5 Vanquish Modules enabling Delay Volume Determi...

Page 8: ...le Wash Liquid Guidelines 159 7 7 2 Replacing Needle Wash Lines 160 7 7 3 Performing A Needle Wash Cycle 162 7 8 Flush System 164 7 8 1 Flush Solvent Guidelines 164 7 8 2 Replacing Flush Purge Lines 165 7 8 3 Performing A Flush Purge Cycle 171 7 9 Replacing the Needle Capillary 172 7 10 Replacing the Needle Unit 177 7 11 Replacing the Drain Pump Head with Tubing 181 7 12 Replacing the Fraction Col...

Page 9: ...t Be Achieved 214 9 Specifications 216 9 1 Performance Specifications 217 9 2 Physical Specifications 220 10 Accessories Consumables and Replacement Parts 222 10 1 General Information 223 10 2 Ship Kit 224 10 3 Optional Accessories 226 10 4 Consumables and Replacement Parts 228 11 Appendix 232 11 1 Compliance Information 233 11 1 1 Declarations of Conformity 233 11 1 2 WEEE Compliance 234 11 1 3 F...

Page 10: ...Fraction Collector FT VF F20 A Operating Manual 1 Using this Manual This chapter provides information about this manual the conventions used throughout the manual and the reference documentation that is available in addition to this manual ...

Page 11: ...erly Note the following The device configuration may vary therefore not all descriptions necessarily apply to your particular device If some detail applies to only one model or variant the model or variant is identified by name The descriptions in this manual refer to a Vanquish system configuration with optical detector Not all descriptions necessarily apply to your particular system Illustration...

Page 12: ...ection to which they apply Safety messages that apply to only a particular section or procedure appear in the section or procedure to which they apply They appear different from the main flow of text Safety messages are often preceded by an alert symbol and or alert word The alert word appears in uppercase letters and in bold type Make sure that you understand and follow all safety messages presen...

Page 13: ...screen Commands that you enter by the keyboard Names of for example dialog boxes properties and parameters For brevity long expressions and paths appear in the condensed form for example Click Start All Programs Thermo Chromeleon 7 Services Manager Start Instrument Controller References and Messages References to additional documentation appear italicized Messages that appear on the screen are ide...

Page 14: ...nual Electronic Manual Version PDF The electronic version PDF of the manual contains numerous links that you can click to go to other locations within the manual These include Table of contents entries Index entries Cross references in blue text for example to sections and figures ...

Page 15: ...t is required Go to the following web site www thermofisher com HPLCmanuals Software Documentation Additional software documentation includes the following Chromeleon Help and documents The Chromeleon Help provides extensive information and comprehensive reference material for all aspects of the software In addition the following documentation is available availability depends on the software vers...

Page 16: ...0 A Operating Manual TIP The Chromeleon Help and documents are included in the software shipment Third Party Documentation Refer also to the user documentation provided by the manufacturers of third party components and materials for example Safety Data Sheets SDSs ...

Page 17: ...1 Using this Manual Integral Fraction Collector FT VF F20 A Operating Manual Page 17 ...

Page 18: ...2 Safety Page 18 Integral Fraction Collector FT VF F20 A Operating Manual 2 Safety This chapter provides general and specific safety information and informs about the intended use of the device ...

Page 19: ...AUTION Indicates a hazardous situation that if not avoided could result in minor or moderate injury WARNING Indicates a hazardous situation that if not avoided could result in serious injury 2 1 2 Observing this Manual Observe the following Before installing or operating the device read this manual carefully to be familiar with the device and this manual The manual contains important information w...

Page 20: ...cates a potential pinch point hazard Keep your hands clear to avoid harming your hands 2 1 4 Applicable Symbols on the Device Symbol Description Need to remove the shipment lock before powering on the device Consult instructions for use 2 1 5 Rating Plate The rating plate is present on the device near the electrical connections The rating plate indicates the serial number part number module name r...

Page 21: ...laboratory environment only The device and Vanquish system are intended to be used as General Laboratory Equipment GLE They are not intended for use in diagnostic procedures Laboratory Practice Thermo Fisher Scientific recommends that the laboratory in which the Vanquish system is used follow best practices for LC analyses This includes among others Using appropriate standards Regularly running ca...

Page 22: ...components options and peripherals specifically authorized and qualified for the device by Thermo Fisher Scientific Perform only the procedures that are described in this operating manual and in supporting documents for the device Follow all instructions step by step and use the tools recommended for the procedure Open the enclosure of the device and other components only if specifically instructe...

Page 23: ...he module the installer is responsible for ensuring the safety of the module and system General Operation The device is designed to be operated only by trained and qualified personnel in a laboratory environment All users must know the hazards presented by the device and the substances they are using All users should observe the related Safety Data Sheets SDSs 2 3 3 Personal Protective Equipment W...

Page 24: ...ectric Shock or Damage to the device High voltages are present inside the device that could cause an electric shock or damage to the device Do not make any changes to the electrical or grounding connections Do not remove the grounding connections and always keep the grounding connections in good contact If you suspect any kind of electrical damage disconnect the power cord and contact Thermo Fishe...

Page 25: ...ce the volume of substances to the minimum volume required for sample analysis Do not operate the device in a potentially flammable environment Avoid accumulation of harmful substances Make sure that the installation site is well ventilated Dispose of hazardous waste in an environmentally safe manner that is consistent with local regulations Follow a regulated approved waste disposal program WARNI...

Page 26: ...tallation site is well ventilated Avoid open flames and sparks Do not operate the device in the presence of flammable gases or fumes WARNING Flammable and Hazardous Vapors Flammable or hazardous vapors can escape from improperly sealed sample containers with flammable or volatile samples and can accumulate inside the device This can pose health and safety risks and lead to wrong results Observe th...

Page 27: ...nformation refer to the technical literature on the chemical resistance of PEEK CAUTION Allergic Reaction Some capillaries in the Vanquish system are made of MP35N a nickel cobalt based alloy Individuals with sensitivity to nickel cobalt may show an allergic reaction from skin contact CAUTION Sparking due to Electrostatic Discharge Liquid flowing through capillaries can generate static electricity...

Page 28: ...Integral Fraction Collector pH values of 2 or less The application time should be as short as possible Flush the system thoroughly after these applications pH values higher than 9 5 with optical detectors Avoid using mobile phases with a pH value higher than 9 5 together with optical detectors This can impair the functionality and optical performance of the detector flow cell pH values higher than...

Page 29: ...sure Vanquish Horizon Vanquish Flex 1 mol L or less 2 4 4 Further Information For details about the materials that are used in the analytical flow path of the device see the Specifications chapter in this manual For information about the materials that are used in the flow path of the other modules in the Vanquish system refer to the Specifications chapter in the Operating Manual for the modules F...

Page 30: ...ges that you make to the device may void compliance with one or more of these EMC and safety standards Changes to the device include replacing a part or adding components options or peripherals not specifically authorized and qualified for the product by Thermo Fisher Scientific To ensure continued compliance with EMC and safety standards replacement parts and additional components options and per...

Page 31: ...2 Safety Integral Fraction Collector FT VF F20 A Operating Manual Page 31 ...

Page 32: ...3 Device Overview Page 32 Integral Fraction Collector FT VF F20 A Operating Manual 3 Device Overview This chapter introduces you to the device and the main components ...

Page 33: ... including septum piercing Support of different collection path mode Vertical SawVertical Horizontal and SawHorizontal Automated delay volume determination in Chromeleon CDS Washing of the outer needle surface and rinse of inner needle surface for minimum fraction collection carry over The sample flow path is built with biocompatible components Temperature control is available in the fraction coll...

Page 34: ...the start of a fraction collection the fraction collection valve is switched to collect position under consideration of the delay volume between the detector outlet and needle tip to direct the flow into the collection sample container The needle can be programmed to stay above the sample container or descend into the sample container during fraction collection When the CDS triggers a tube change ...

Page 35: ...e will be switched to waste position until a new fraction collection is triggered To remove residual sample from the needle external between runs the needle moves to the wash port and descends into it The needle wash pump supplies needle wash liquid to the wash port to wash the outer surface of the needle to the wash waste To remove residual fraction from needle internal between runs the needle mo...

Page 36: ...ion valve 7a Needle wash liquid 3 Needle capillary 8 Flush solvent 4 Needle unit 9 Flush pump 5 Carousel with collection vials 10 Flush buffer loop 6 Needle wash port 11 Air bubble sensor 6a Needle wash waste 12 Y connector Note Waste drainage is routed to a waste container The following figures illustrate how the device operates for collecting fractions flushing between fractions of one run washi...

Page 37: ...Page 37 purging the flush pump The fraction collection valve is switched between three different positions depending on the operation Figure 3 Fraction collection with fraction collection valve in collect position Figure 4 Flush with fraction collection valve in flush position ...

Page 38: ...3 Device Overview Page 38 Integral Fraction Collector FT VF F20 A Operating Manual Figure 5 External Needle Wash in needle wash port with fraction collection valve in waste position ...

Page 39: ...e 6 Internal Needle Rinse in needle wash port with fraction collection valve in collect position Several draw and dispense strokes are executed during the purge flush pump operation to fill the flow path from flush solvent container flush pump to flush buffer loop with fresh flush solvent ...

Page 40: ...iew Page 40 Integral Fraction Collector FT VF F20 A Operating Manual Figure 7 Draw of fresh flush solvent from solvent container to fill flush pump Figure 8 Dispense liquid out to flush buffer loop and drain to waste ...

Page 41: ...ible components of the device are located directly behind the front doors Figure 9 Interior view No Description 1 Keypad with status indicators 2 Fraction collector compartment with carousel 3 Removable front plate 4 Front leak tray 5 Type label indicating the part number module name and serial number 6 Right interior side with flow components ...

Page 42: ...g and drain pump waste tubing 2 Needle capillary securing plate 9 Drain pump 3 Needle wash port 10 Flush pump 4 Needle wash pump installed behind the panel 11 Y connector PEEK 5 Guide hole for capillary from other module 12 Flush buffer loop connecting between fraction collection valve port 1 and port 4 6 Guide hole for wash port outlet tubing and condensation tubing 13 Fraction collection valve 7...

Page 43: ...r the fraction collector compartment Circulating air is used to cool or heat the fraction collector compartment to the set temperature thus allowing precise equalization of the sample temperature To achieve an optimum thermostatting performance keep the front doors of the device closed during fraction collection Open the front doors only if required for example to load the device with sample conta...

Page 44: ...Description 1 Positioning area Space to position a sample rack or well plate 2 Barcode label empty Label that identifies that the segment is empty for the barcode reader 3 Alignment frame Angles to align the sample rack or well plate within easily 4 Color code for the segment here R for the red segment 3 4 3 Rack Type Identification A barcode reader inside the fraction collector compartment allows...

Page 45: ... rack with barcodes for rack type identification No Description 1 Position A1 2 Vanquish rack type barcode 3 4 4 Condensation Drainage A leak sensor is embedded inside the fraction collector compartment The sensor detects liquid accumulated inside the fraction collector compartment due condensing water when the device is operated in cooling mode under humid conditions If liquid is detected the dra...

Page 46: ...uid is collected in the front leak tray and guided to the drain port From the drain port the liquid is guided to waste through the drain system of the Vanquish system When the front leak sensor detects leakage the status indicators change to red and beeping starts to alert you Follow the instructions in this manual to find and eliminate the source for the leakage For more details see Resolving Fro...

Page 47: ...ment control data acquisition and data management For general information how to set up a system in Chromeleon refer to the Chromeleon Installation Guide For information about setting up the Vanquish system in Chromeleon refer to the Vanquish System Operating Manual Details on control and operation of the device are available in the Chromeleon Help A keypad is available inside the device allowing ...

Page 48: ...3 Device Overview Page 48 Integral Fraction Collector FT VF F20 A Operating Manual ...

Page 49: ...ng and Transport Integral Fraction Collector FT VF F20 A Operating Manual Page 49 4 Unpacking and Transport This chapter provides information for unpacking the device and informs you about the scope of delivery ...

Page 50: ...ulky for one person alone to handle safely To avoid personal injury or damage to the device observe the following guidelines Physical handling of the device including lifting or moving requires a team effort of two persons A team effort is in particular required when lifting the device into the system stack or when removing it Use the carrying handles that were shipped with the device to move or t...

Page 51: ...evice by the carrying handles to the installation site if it is not already there and place it in the system stack See System Arrangement Page 63 7 On each carrying handle loosen the attachment screw until the carrying handle is moveable in the rail Do not remove the screws from the carrying handles completely 8 Slide off the carrying handles from the rails towards the rear of the device Figure 14...

Page 52: ... shipping locks or protective covers from the fraction collector as required Shipping locks are marked for removal Remove the shipping locks and protection foams 1 Remove two white protective foams from R and G segments of the carousel Figure 15 Remove two carousel protection foams 2 Remove the shipping lock fixing needle arm Unscrew three screws on the shipping lock completely with the screwdrive...

Page 53: ...4 Unpacking and Transport Integral Fraction Collector FT VF F20 A Operating Manual Page 53 3 Remove the shipping lock fixing carousel Figure 17 Remove carousel shipping lock ...

Page 54: ...tegral Fraction Collector FT VF F20 A Operating Manual 4 2 Scope of Delivery The following items are included in the delivery Fraction collector Ship kit For details about the kit content see Ship Kit page 224 Safety notes and test report ...

Page 55: ...tor FT VF F20 A Operating Manual Page 55 5 Installation This chapter specifies the requirements for the installation site and describes how to set up install and configure the device in the Vanquish system and in the chromatography software ...

Page 56: ...e including lifting or moving requires a team effort of two persons A team effort is in particular required when lifting the device into the system stack or when removing it Use the carrying handles that were shipped with the device to move or transport the device Never move or lift the device by the front doors This will damage the doors or the device CAUTION Electric Shock or Damage to the Devic...

Page 57: ...up the device hardware See Setting Up the Hardware page 63 3 Set up the flow connections See Setting Up the Flow Connections page 68 4 Turn on the device TIP Before turning on the power to a Vanquish system module for the first time verify that the chromatography software is installed on the data system computer When the power is turned on the required USB drivers are automatically found and the W...

Page 58: ...alled in the Vanquish system prepare the device for transport and move it to the new location Follow the instructions in Transporting or Shipping the Device page 193 TIP When the power is turned off to the device the left front door of the device is opened automatically for proper ventilation of the fraction collector compartment and cannot be closed while the power is turned off ...

Page 59: ...iderations The power supply of the device has wide ranging capability accepting any line voltage in the range specified for the device CAUTION Electric Shock or Damage to the device Connecting the device to a line voltage higher or lower than specified could result in personal injury or damage to the device Connect the device to the specified line voltage only Never connect the device to a power s...

Page 60: ...e a power cord that is designed for the country in which you use the device Do not use extensions cords Never plug the power cord to a power socket that is shared with other equipment for example multiple sockets Operate the device only from a power outlet that has a protective ground connection In case of emergency it must be possible to reach the power cord easily at any time to disconnect the d...

Page 61: ...ing the device avoid or minimize conditions that can lead to a build up of condensation in the device For example avoid significant or fast changes in environmental conditions If you suspect that condensation is present allow the device to warm up to room temperature This may take several hours Wait until the condensation is gone completely before connecting the device to the power line ...

Page 62: ...vice are located directly behind the doors Figure 18 Opening the front doors Front Door Opening Mechanism The left front door of the device is equipped with a mechanism that opens the door automatically for proper ventilation of the fraction collector compartment when the device is turned off When the device is turned on the left front door can be closed If a power failure occurs or if the power c...

Page 63: ...are arranged in a system stack with the arrangement depending on the system configuration and the lab environment For reducing the overall height of the system a two stack configuration is recommended Two stacks require a second System Base A second Solvent Rack can be added optionally Figure 19 Vanquish system standard configuration examples left one stack right two stacks No Description No Descr...

Page 64: ...tilting system stack As for one stack configuration the overall height exceeds 100 cm It is required to use a stack stabilizer kit to prevent any potential risk of tilting for the one stack configuration For instructions how to set up the system stack refer to the Vanquish System Operating Manual ...

Page 65: ...mber if any line and fuse rating and the manufacturer s address 2 Main power switch on off control 3 Fuse holder 4 Power inlet connector 5 System Interlink port Only allows power on off control for the device from the Vanquish system base and device communication so far 6 Digital I O ports Dig I O Allow exchange of digital signals with external instruments Each digital I O port provides one input ...

Page 66: ... system or the computer on which the data management system is installed such as the Chromeleon software TIP Thermo Fisher Scientific recommends using the USB ports only as described above If the USB ports are used for any other purpose Thermo Fisher Scientific cannot ensure proper functionality Connecting the USB and system interlink cables Figure 21 USB and system interlink cable connections ...

Page 67: ...onnect the required interface cables to the device For information about how to connect the device to other modules in the Vanquish system or to the chromatography data system computer refer to the Vanquish System Operating Manual 3 Verify that the power switch on the device is set to OFF 4 Connect the power cord to the power inlet connector on the device NOTICE Before connecting the device to the...

Page 68: ...d precautionary statements presented in Safety Precautions page 22 Dirty components can contaminate the chromatography system Contamination leads to poor performance of the modules and entire system or can even cause damage to the modules and system Therefore Always wear appropriate gloves Place the components only on a clean lint free surface Keep your tools clean Use only lint free cloth for cle...

Page 69: ... wash reservoir See Connecting the Needle Wash Reservoir page 81 3 Set up the fraction collection valve connections See Fraction Collection Valve page 85 5 6 2 Guiding Capillaries and Tubing Through the System Flow connections between the modules of the Vanquish system are guided through either the tubing chase in the devices or the guide holes or capillary clips of the devices Tubing Chase Tubing...

Page 70: ...al wash fraction collector needle wash 4 Detector and fraction collector waste lines Tubing brackets are available for holding the tubing in place Slip the bracket side onto the drain pipe Figure 23 Tubing bracket left tubing bracket installed right Guide Holes and Capillary Clips Guide holes and capillary clips are provided at specific positions on the system modules Route flow connections from o...

Page 71: ...hermo Fisher Scientific The connectors must be free from contaminants Even minor particles may cause damage to the system or lead to invalid test results Do not install capillaries or tubing that is stressed nicked kinked or otherwise damaged Install capillaries and fittings only at the positions for which they are intended 5 6 3 2 Overall Flow Path Connection A delay capillary is used to connect ...

Page 72: ... path connection are as follows No Description Shipped with 1 0 10 x 350 mm MP35N System Base Vanquish Horizon Flex 2 Active or passive pre heater MP35N System Base Vanquish Horizon Flex 3 Depending on detector 0 10 x 300 mm MP35N insulated or Post Cooler 0 10 x 300 mm MP35N 0 10 x 350 mm MP35N 0 10 x 450 mm MP35N Diode Array Detector HL Diode Array Detector FG Variable Wavelength Detector F Fluor...

Page 73: ...x 415 mm PEEK Integral Fraction Collector FT Needle capillary for 5 10 mL min 0 25 x 415 mm PEEK Optional needs be ordered separately 6 Depending on flow rate Flush buffer loop for up to 5 mL min 50 µL PEEK Integral Fraction Collector FT Flush buffer loop for 5 10 mL min 100 µL PEEK Optional needs be ordered separately 7 Waste fluidic Detector For a one stack configuration with a Vanquish Core HPL...

Page 74: ... 5 mL min 0 18 x 1200 mm MP35N 0 5 1 mL min 0 25 x 1500 mm MP35N 1 2 mL min 0 5 x 800 mm PEEK 2 10 mL min 1 x 1000 mm PEEK Optional needs be ordered separately 5 Depending on flow rate Needle capillary for up to 5 mL min 0 18 mm PEEK Integral Fraction Collector FT Needle capillary for 5 10 mL min 0 25 mm PEEK Optional needs be ordered separately 6 Depending on flow rate Flush buffer loop for up to...

Page 75: ... MP35N System Base Vanquish Horizon Flex 3 Depending on flow rate Up to 2 mL min 0 1 x 550 mm MP35N 2 10 mL min 0 18 x 550 mm MP35N Integral Fraction Collector FT 4 Depending on fraction collection mode and flow rate Delay capillary for time based fractionation Up to 2 mL min 0 1 x 350 mm MP35N 2 10 mL min 0 18 x 350 mm MP35N Integral Fraction Collector FT Delay capillaries for peak based fraction...

Page 76: ...ption Shipped with 1 0 18 x 350mm SST System Base Vanquish Core 2 Passive pre heater SST System Base Vanquish Core 3 Depending on flow rate Up to 2 mL min 0 1 x 550 mm MP35N 2 10 mL min 0 18 x 550 mm MP35N Integral Fraction Collector FT 4 Depending on fraction collection mode and flow rate Delay capillary for time based fractionation Up to 2 mL min 0 1 x 350 mm MP35N 2 10 mL min 0 18 x 350 mm MP35...

Page 77: ...ibes how to connect Viper capillaries All Viper flow connections in the Vanquish system are designed to be finger tight To connect Viper capillaries with knurl follow these steps NOTICE Tighten or loosen Viper capillaries only with your fingers Do not use tools other than the knurl that comes with the capillary To avoid damage to the capillary or connection tighten and loosen the Viper capillaries...

Page 78: ...ree tissue wetted with isopropanol 5 Reinstall the capillary 6 If the connection continues to leak install a new Viper capillary 5 6 4 Guiding Liquids to Waste Waste liquids from the needle wash system and the drain pump in the device are routed through separate drain ports directly into the drain system Leaking liquids of the device are collected in the leak tray and flow off through the funnel a...

Page 79: ...t for needle wash system 2 Drain port for drain pump 3 Funnel to drain system 4 Waste line from the device Y connector to waste container TIP If you have to cut tubing to length use a tubing cutter Make sure that the cut is at right angle to the length of the line The waste line should go straight to the system base and to waste Make sure that the line is positioned straight in the tubing guides ...

Page 80: ... needle wash lines needle wash port and waste line When the device is shipped the needle wash components are installed within the device Figure 28 Needle wash components in the device No Description 1 Needle wash inlet line up to needle wash reservoir 2 Needle wash port 3 Needle wash outlet line from wash pump outlet to needle wash port 4 Needle wash waste line 5 Needle wash pump installed behind ...

Page 81: ...dle sufficiently After shipment of the device Check if the needle wash waste line is properly connected to the drain port and has not popped out during shipment of the device 5 7 2 Connecting the Needle Wash Reservoir Parts and tools required Needle wash reservoir Needle wash inlet line the silicon tubing Retaining guide 1 8 Reservoir cap Cap plugs Tubing cutter optional Preparations Prepare the n...

Page 82: ...e Fraction Collector 1 Connect the needle wash line to the needle wash pump outlet port in the device Figure 29 Needle wash line connection No Description 1 Needle wash line up to needle wash reservoir 2 Route the needle wash inlet line from the device to the solvent rack through the tubing guides of the modules and the guide hole in the solvent rack Figure Guide hole and tubing guides in the solv...

Page 83: ...eservoir thoroughly with a high purity solvent b Fill the needle wash reservoir with fresh needle wash liquid c Feed the needle wash line through the retaining guide and through an open hole in the cap of the needle wash reservoir The retaining guide keeps the tubing in place in the reservoir Close any open holes in the reservoir cap with cap plugs Figure 30 Securing the needle wash line with the ...

Page 84: ... straight in the tubing guides 6 Check the needle wash line across the entire flow path a Make sure the line is not bent pinched or squeezed at any point in the flow path b If you have to cut tubing to length use a tubing cutter Make sure that the cut is at right angle to the length of the line Purging the Needle Wash System After the device has been turned on purge the needle wash system using Ch...

Page 85: ...unit inside fraction collector compartment 5 8 1 Port assignments of the Fraction Collection Valve Figure 31 Fraction collection valve with connected capillaries except port 6 The fraction collection valve ports are assigned as follows Port Connected Component Port Connected Component 1 to flush buffer loop pre installed 5 to Y connector pre installed 2 to needle Capillary 6 Central port inlet fro...

Page 86: ...on and that removes residual fraction from the flush buffer loop sufficiently 5 9 2 Connecting Flush Solvent Reservoir Parts required Flush solvent reservoir Flush solvent tubing Retaining guide 1 16 Solvent line filter Filter frit Solvent line adaptor 1 8 1 16 Reservoir cap Cap plugs Preparations 1 Prepare the flush solvent and flush solvent reservoir Observe the flush solvent guidelines in Choos...

Page 87: ...nt line filter top holder 4 Solvent line adapter 1 16 Follow these steps Setting up the flush system comprises the following steps 1 Setting up the flush solvent tubing in the fraction collector 2 Connecting the flush solvent reservoir 3 Purging the flush pump Follow the steps in the respective sections further down Setting up the Flush Solvent Tubing in the Fraction Collector 1 Connect the flush ...

Page 88: ...e flush solvent tubing from the device to the solvent rack through the tubing guides of the modules and the guide hole in the solvent rack Figure 34 Guide hole and tubing guides in the solvent rack No Description A Guide hole 1 2 Not to be used for wash liquid line reserved for other tubing 3 Tubing guide for wash liquid line 4 Not to be used for wash liquid line reserved for other tubing ...

Page 89: ...place in the reservoir Close any open holes in the reservoir cap with cap plugs Figure 35 Securing the flush solvent tubing with the reservoir cap 2 Close any open holes in the reservoir cap with cap plugs 3 Slide the filter holder and adapter onto the end of the flush solvent tubing 4 Tighten the reservoir cap hand tight Press the retaining guide into the hole in the reservoir cap to ensure that ...

Page 90: ...After the device has been turned on purge the flush pump using Chromeleon to fill the flush pump with the fresh flush solvent During purging the flush pump and flush buffer loop are washed continuously until all flush purge lines are filled with the fresh flush solvent 5 9 3 Connecting the Delay Capillary Parts required Delay Capillary Follow these steps 1 Remove the detector waste line if it has ...

Page 91: ...tion panel of the detector 4 Route the delay capillary between detector and the fraction collector depending on length of the delay capillary that is used 5 9 4 Connecting the Flush Buffer Loop Parts required Needle capillary TIP Make sure to check the flow rate of application and choose the proper needle capillary according to application flow rate range Meanwhile the installed flush buffer loop ...

Page 92: ...d separately Tools required Screwdriver Torx T10 NOTICE The device shall be powered off while replacing the needle capillary Follow these steps Install the needle capillary See Replacing the Needle Capillary page 172 NOTICE Make sure no extra stress damage and kink defects for needle capillary during the installation process ...

Page 93: ...und and the Windows operating system can detect the device To turn on the power to the device follow these steps 1 Check that the power button on the front left of the Vanquish system base system power button is pressed in If the power button stands out press the power button to turn on the power on the system base 2 Turn on the device with its main power switch Turn the device off with the main p...

Page 94: ...illary The NeedleCapillary property must be set correctly in the software according to needle capillary installed on device see Connecting Fittings Capillaries and Tubing page 71 The needle capillary included in the default shipment is ID 0 18 mm This is also the default setting for needle capillary in the Chromeleon If the needle capillary with ID 0 25 mm is installed the new capillary dimensions...

Page 95: ...eCapillary parameter in Property and check if the correct needle capillary is set and select the correct needle capillary if required The capillary marker on the needle capillary indicates the dimensions Figure 39 Set the type of needle capillary For more details see Optional Accessories Page 226 ...

Page 96: ... Operation Page 96 Integral Fraction Collector FT VF F20 A Operating Manual 6 Operation This chapter describes the elements for device control provides information for routine operation and for shutdown ...

Page 97: ...e case refer to the instructions in Installation page 55 before proceeding For a basic description of instrument control and automated sample analysis with the Chromeleon software refer to the Vanquish System Operating Manual Details on control and operation of the device are available in the Chromeleon Help Software descriptions in this manual refer to Chromeleon 7 Terminology may be different to...

Page 98: ...ors from Spills Flammable or hazardous vapors from sample spills can accumulate inside the device This can pose health and safety risks Ensure that the sample racks and well plates are properly positioned in the segments When you use sample racks fill the sample rack with the vials before placing the sample rack in the carousel Do not open the device front doors and remove any sample racks or coll...

Page 99: ...he needle arm and carousel with fraction collection valve switched to waste position as well If the doors are not closed within two minutes then the sequence will stop too If the doors are closed within two minutes the fraction collection processing will continue by following the right moment collection requirement from the system When operating the chromatography system always set the lower press...

Page 100: ... the STATUS LED on the keypad provide a quick visual check of the operational status of the device 6 3 1 Keypad The keypad inside the device allows you to perform certain functions directly from the device When you press a button a short beep confirms that the function is performed When the device is connected in the Chromeleon software some functions may not be available from the keypad see furth...

Page 101: ... Pressing the SERVICE button a second time allows the device to move needle to parking position VALVE The VALVE button allows switching the fraction collection valve between Waste and Collect position The LEDs next to the VALVE button indicate the position of the fraction collection valve when they are illuminated green If both LEDs are off it means the fraction collection valve is in Flush positi...

Page 102: ...ble from the keypad allowing you to turn off the beep for the current alarm In addition the LIGHT button remains available from the keypad allowing you to control the cabin light All other buttons are disabled The device is running a manual flush purge function The VALVE and SERVICE buttons cannot not be operated 6 3 2 Status Indicators The status LED bar on the front side of the device and the ST...

Page 103: ...emperature is achieved Blue running The device performs fraction collecting while a sequence is running Blue A sample or sequence is running including data acquisition Red A problem or error has occurred For the related message check the Chromeleon Audit Trail For remedial action see the Troubleshooting section in this operating manual STATUS LED The STATUS LED on the left side of keypad provides ...

Page 104: ...en the power is off the system power button stands out If the main power switch on a device is off you cannot turn on the device with the system power button To turn off a device completely you have to turn it off with the main power switch on the device Pressing the system power button will not be sufficient to turn off the power to the device completely Upon power up the device performs a self t...

Page 105: ...e components of the device are filled with isopropanol when the device is shipped from the manufacturing site When operating the device for the first time use solvents that are miscible with isopropanol If they are not use an appropriate intermediate solvent Inspect components and fluidic connections prior to fluidic operation 1 Verify that all necessary solvents are available in the solvent rack ...

Page 106: ...n solvent is ready in both flush pump and flush buffer loop See Flush System Page 164 Flushing the entire chromatography system with the starting solvent to rinse out any solvent from a previous analysis run In Chromeleon run a manual needle internal rinse cycle with the starting solvent to rinse out any other liquids from the internal surface of needle capillary and needle of the fraction collect...

Page 107: ...trol To thermostat the fraction collector compartment enable the temperature control Temperature Control On Target temperature Define the temperature setpoint to which the fraction collector compartment is to be cooled or heated Temperature Nominal Observe the following Ensure that the thermostatting temperature is suitable for your samples and within the specified temperature range of the device ...

Page 108: ...ootprint Rack type identification To allow the identification of the sample rack or well plate type with the barcode reader in the device use sample racks and well plates with such barcodes for rack type identification Figure 41 Sample rack with barcodes for rack type identification No Description 1 Position A1 2 Vanquish rack type barcode TIP For ordering information of sample racks and well plat...

Page 109: ...type barcodes to the carousel the rack type and rack orientation will not be identified automatically It may be helpful to write down the rack types that are installed in the carousel to enter them manually in Chromeleon Follow these steps 1 Rotate the desired segment of the carousel to the front You can rotate the carousel in the following ways Select the Rotate button on the keypad Pressing the ...

Page 110: ...2 Correct orientation in the carousel here with a sample rack 3 Rotate to the next loading position and load it as required When you have loaded the segments as required continue with the next step 4 Close the front doors of the device The device starts an inventory scan of the sample racks or well plates in the fraction collector compartment 5 Continue as required by the sample racks or well plat...

Page 111: ...ification or rack orientation is available Make sure that the sample rack or well plate is installed in correct orientation The rack type will be identified as Unknown Manually select the rack types for each segment in Chromeleon To change a sample rack or well plate use the Change Rack command for the respective segment in Chromeleon For details on the rack type settings refer to the Chromeleon H...

Page 112: ... 10 45 WellPlate96 n a 10 50 3 3 41 5 WellPlate384 n a 10 50 3 3 41 5 For AboveVial mode the default needle height value considers a distance of 2 mm between the height of the vial and the needle tip to ensure no needle obstruction can happen For the use of well plates in AboveVial mode the plate height must be configured into the system manually For details of needle positioning mode settings ref...

Page 113: ...remove a sample rack or well plate Tube position The tube position identifies the position into which the next fraction is collected The Tube Position consists of the color code for the segment and the position on the sample rack or well plate which are separated by a colon for example B E8 for segment B sample position E8 Reset start position Set the start position to the first available position...

Page 114: ...example after replacing the needle wash liquid you can select the Purge Needle Wash button on the ePanel FC tab for the device Chromeleon turns on the needle wash pump and performs a needle wash cycle with dedicated wash settings for purging the needle wash system After purging the needle wash pump is turned off again and the previous wash settings are restored As a standard the needle wash pump i...

Page 115: ...s a standard the wash mode is set to No Wash in Chromeleon Rinse mode The rinse mode defines if and when an automatic needle rinse is to take place during the collecting sequence The following rinse modes are available No Rinse The needle rinse in the needle wash port is not part of the collecting injection Before Collection The needle is rinsed at the beginning of the collecting injection End of ...

Page 116: ...s to waste position when the needle moves from one vial to next vial Interrupt default In this mode the fraction collection valve switches to waste position when the needle moves to the next vial during which the flow is directed to waste container Continue In this mode the fraction collection valve remains at collection position when the needle moves from vial to vial during which the flow keeps ...

Page 117: ...ecked to keep the needle 2mm above vials based on automatic rack type identification function If this option is checked Needle Height for Vials and Needle Height for Well Plates become read only otherwise they can be changed for optimized heights In Above Vial mode well plate heights range between 10 mm and 50 mm and cannot be automatically recognized so it is necessary to manually input the speci...

Page 118: ...pillary IDxL Select either Custom or predefined Delay Capillary IDxL If the predefined Delay Capillary IDxL is selected the Delay Volume is automatically updated Delay capillary ID Delay capillary L If Custom as Delay Tubing Capillary IDxL is selected then need to manually input Delay Capillary ID and Delay Capillary L The Delay Volume is calculated and displayed accordingly Fraction collection op...

Page 119: ...position whenever a new sample is started This is also referred to as pooling Concatenating Concatenating fraction collection can be enabled by using custom injection variables in Chromeleon sequence view and associate the values to StartFractionPosition and FractionRange properties in the Script Editor of Chromeleon Console Refer to Chromeleon Online Help for details ...

Page 120: ...n collection operation The product provides the feature Automated Delay Volume Determination DVD with which the delay volume can be determined conveniently and set in the system automatically The delay time can be automatically calculated and set in the system based on the delay volume and specified method configuration e g pump flow rate The correct delay time ensures optimal fraction collection ...

Page 121: ...w start Delay time End of fraction collection t t collection time window end Delay time 6 7 2 When to Perform DVD Function It is necessary to re measure the delay volume if any of the below conditions are met Initial installation of a new HPLC system with an Integral Fraction Collector VF F20 A Replacing a detector flow cell Replacing a delay capillary Replacing a fraction collection valve Replaci...

Page 122: ...ater to remove air from the fluidic path 1 Use purified water as mobile phase for running DVD function 2 The procedure requires user intervention after 1 min to install the DVD capillary included in ship kit P N 6040 2330 Viper Cap ID x L 0 13 x 850 mm SST and put an empty vial at position R A1 issued by respective messages in Chromeleon 3 After the DVD capillary was installed and the empty vial w...

Page 123: ...g the measured delay volume in the FC ePanel tab 6 After the successful DVD the method specific delay volume and delay time will be automatically updated in the Instrument Method Wizard The method specific delay time considers the set pump flow rate Figure 45 Method specific delay volume and time ...

Page 124: ...e of the delay capillary volume can only be changed by running DVD or selecting the capillary from the dropdown in Command list F8 If a method specific delay time is set manually it must be larger than 0 Delay volume has to be determined for the first time installation otherwise the fraction collection operation will possibly fail For DVD further information refer to Chromeleon Help and documents ...

Page 125: ...Vanquish model numbers Module Model No Pump Binary Dual Isocratic Quaternary VH P10 A VF P10 A VC P10 A VF P32 A VC P32 A VC P33 A VC P40 A VF P20 A VC P20 A VC P21 A Autosampler VH A10 A VF A10 A VH A40 A VF A40 A VC A12 A VC A13 A Charger VH A90 A Detector Diode Array Variable Wavelength Multi Wavelength VH D10 A VF D11 A VC D11 A VC D40 A VF D40 A VC D12 A Fraction Collector VF F20 A Delay volu...

Page 126: ...age 127 The DVD function is not enabled when an HPLC configuration with two detectors is present If the system configures more than one detector Delay Volume Determination option will not be displayed in the Instrument Qualification Wizard 6 7 6 Vanquish Detector Flow Cells enabling Delay Volume Determination The table below shows the list of Vanquish detector flow cells that enable DVD measuremen...

Page 127: ...uish Fraction Collector in case an automated delay volume determination is not possible due to the presence of a Vanquish Fluorescence Detector or a flow cell in the instrument configuration disabling the delay volume determination measurement It is an independent tool additionally executed besides the Chromeleon 7 Chromatography Data System It is recommended to run the calculator on the same comp...

Page 128: ... 6083 0530A Semi micro flow cell SST 7 mm VF D11 VC D11 VC D12 6083 0540 6083 0540A Standard flow cell biocompatible 10 mm VF D11 VC D11 VC D12 6083 0550A Semi micro biocompatible flow cell MP35N 7 mm VF D11 VC D11 VC D12 The calculator is distributed along with the device firmware and Chromeleon driver in the Vanquish Fraction Collector software package and can be executed from any directory of c...

Page 129: ...he installed Delay Capillary IDxL from the drop down list This will automatically fill the Delay Capillary Diameter and Delay Capillary Length If Custom is selected as Delay Capillary IDxL the values for Delay Capillary Diameter and Delay Capillary Length need to be entered manually 4 Define the Pump Flow Rate to be applied in the instrument method 5 Define the Detector Response Time to be applied...

Page 130: ... 7 Click the Copy button next to the calculated values for Delay Time or Delay Volume 8 Create or open the desired instrument method and paste the value on the Collecting Options page in the Method Specific Delay section Figure 48 Collecting Options page in the instrument method ...

Page 131: ...on Integral Fraction Collector FT VF F20 A Operating Manual Page 131 Figure 49 Pump Flow Rate on Flow Gradient page in instrument method Figure 50 Detector Response Time in Channel Settings in Instrument Method ...

Page 132: ...the sample container is 2 mm with needle tip protruding out of the pusher as the lowest point of the whole needle unit For InVial mode the pusher needs to be the lowest point The purpose is to hold the vial down when the needle is pulled out from the vial septa Between different injections in one sequence the system will detect if the physical pusher position aligns with needle positioning mode If...

Page 133: ...ons 1 Open the left front door 2 Press the service button on keypad to move the needle unit into service position NOTICE To avoid personal injury do not put hands into the compartment when the needle unit is moving Follow these steps 1 Raise the lock pin upwards by hand The pusher will be moved up accordingly Figure 52 Moving the pusher up by hand No Description No Description 1 Lock tab 2 U shape...

Page 134: ...ngage the lock tab with the U shape slot Figure 53 Engaging the lock tab with U shape slot 3 Release the lock pin and check that the needle tip protrudes out of the pusher Then close the left front door Figure 54 Checking the needle tip below the pusher 4 Verify the correct pusher position is set correctly in Chromeleon ...

Page 135: ...personal injury do not put hands into the compartment when the needle unit is moving Follow these steps 1 Raise the lock pin upwards by hand The Lock tab will pop out from the U shape slot accordingly TIP Do not press the lock pin Just lift it up Figure 55 Moving the lock pin up by hand 2 Release the lock pin The pusher is released to InVial position 3 Physically check that the needle tip does not...

Page 136: ...Operation Page 136 Integral Fraction Collector FT VF F20 A Operating Manual Figure 56 Checking the needle tip higher than the pusher tip 4 Verify the correct pusher position is set correctly in Chromeleon ...

Page 137: ...e ensure that the needle is properly cleaned from the remaining last fraction both inside and outside of the needle According to application purpose flush function helps to achieve both higher recovery and minimize carryover Before you switch the fraction collection valve make sure that the pump flow is turned on Avoid switching the fraction collection valve without flow i e switching the fraction...

Page 138: ...ired by your system arrangement TIP Purge the needle wash port with an organic solvent such as isopropanol before you interrupt operation to avoid organic growth in the needle wash system Purge the flush pump to flush buffer loop with an organic solvent such as isopropanol before you interrupt operation to avoid organic growth in the flush buffer loop system Run an internal needle rinse cycle with...

Page 139: ...tion refer to the Operating Manuals for the modules To interrupt operation for a longer period follow the instructions below 1 Check that the fraction collection valve is set to Collect position 2 Flush the system with an appropriate pure solvent minimum HPLC grade Observe the following Situation after Shutdown If no additive is used If an addititive is used Device remains in the laboratory after ...

Page 140: ... shall be transported or shipped after shutdown Flush the needle wash system with an organic needle wash solvent such as isopropanol before you interrupt operation to avoid organic growth in the needle wash system Purge flush pump to flush buffer loop with an organic solvent such as isopropanol before you interrupt operation to avoid organic growth in the flush buffer loop Run an internal needle r...

Page 141: ...roper ventilation of the fraction collector compartment and cannot be closed while the power is turned off Restarting the device after long term shutdown To restart the device follow these steps 1 Remove all shipping protective parts and connecting required capillaries and tubing if the protective parts were installed and fluidics uninstalled before For details see Unpacking and Transport page 49 ...

Page 142: ...nance and Service Page 142 Integral Fraction Collector FT VF F20 A Operating Manual 7 Maintenance and Service This chapter describes the routine maintenance and the service procedures that the user may perform ...

Page 143: ...c for brevity referred to as Thermo Fisher Scientific service personnel The device is designed for easy maintenance and service The user serviceable parts of the device can be accessed from the front If not stated otherwise the maintenance procedures do not require that you remove the device from the system The maintenance procedures do not require that you remove the doors However it is possible ...

Page 144: ...jury to the skin To avoid personal injury never touch the needle tip WARNING Tilting Liquid Reservoirs Liquids in the reservoirs on the solvent rack might contain harmful substances Spilling of these substances can pose health and safety risks To prevent the reservoirs from tilting be careful not to pull on the liquid lines when performing maintenance WARNING Escape of Hazardous Substances from Fl...

Page 145: ...ressure flow path Turn off the pump flow and wait until the system pressure is down to zero Unscrew the caps of the solvent reservoirs and remove the solvent lines together with the caps from the reservoirs Empty the solvents lines Refer to the Operating Manual for the pump Retighten the reservoir caps CAUTION Electric Shock or Damage to the Device After the power to the device is turned off the d...

Page 146: ...Collector FT VF F20 A Operating Manual TIP When the power is turned off to the device the left front door of the device is opened automatically for proper ventilation of the fraction collector compartment and cannot be closed while the power is turned off ...

Page 147: ...ath to replace capillaries in the system turn off the pump flow and wait until the system pressure is down to zero Dirty components can contaminate the chromatography system Contamination leads to poor performance of the modules and entire system or can even cause damage to the modules and system Therefore Always wear appropriate gloves Place the components only on a clean lint free surface Keep y...

Page 148: ...s Check the liquid level of the needle wash and flush solvent reservoir Fill the solvent reservoirs with fresh liquid if required Purge needle wash system to fill the needle wash port with the fresh needle wash liquid During purging the needle wash port is flushed continuously until the fresh needle wash liquid is present In Chromeleon purge the flush pump to remove potential air in flush fluidics...

Page 149: ...placement Annually Have Thermo Fisher Scientific service personnel perform preventive maintenance once a year TIP Chromeleon supports functions for estimating the lifetime of consumables See Predictive Performance page 151 7 4 2 Cleaning or Decontaminating the Device Cleaning and decontamination must be performed by qualified personnel wearing suitable personal protective equipment Always observe ...

Page 150: ... system Never use sharp tools or brushes for cleaning any surfaces Do not use sprays for cleaning Prevent cleaning detergent from entering the flow path Do not use excessively wetted cloth or wipes for cleaning Prevent any liquids from entering the functional components of the device Liquids can cause a short circuit when getting in contact with the electronic components Preparations Open the left...

Page 151: ...e These functions which are called Predictive Performance allow you to schedule maintenance procedures based on the actual operating and usage conditions of the device On special wellness service and qualification panels you can define intervals for replacing components that are subject to wear or stress and for service procedures or qualification procedures In addition you can set limits to alert...

Page 152: ...e Parameters for the Fraction Collector The list shows the Predictive Performance counter for the device in Chromeleon Consider resetting the parameter after performing the related maintenance procedure Predictive Performance Command To perform ServiceDone After annual maintenance by service personnel ...

Page 153: ...ution is part of the mobile phase flush the device with several volumes of methanol water 50 50 before shutting down the device 3 Turn off the pump flow Wait until the system pressure is down to zero before you continue the shutdown of the device 4 Empty the flush purge system a Unscrew the cap of the flush solvent reservoir b Together with the cap remove the flush solvent tubing from the reservoi...

Page 154: ... 7 5 2 Securing the Needle Drive When Before maintenance procedures or for transport make sure to secure the needle drive NOTICE If the needle drive is not secured during maintenance or transport it can damage the device Secure the needle drive when instructed to do so to avoid damage to the device When the device is powered off the needle drive may move inside the fraction collector compartment w...

Page 155: ...ement if case of broken front plate cable Parts required in case repair Replacement front plate Tools required Screwdriver Torx T10 Preparations Turn off the device with its main power switch NOTICE The device shall be powered off while replacing the front plate Follow these steps 1 Loosen the two screws that attaches the removable front plate with the screwdriver The screws shall remain in the fr...

Page 156: ...Description 1 Front plate 2 Screws 2 Pull the front plate outward and remove it from the slide in module Figure 58 Pulling out the front plate 3 Pull out the cable connector from the front plate which is located at the top Disconnect the cable connector Figure 59 Disconnecting the cable connector ...

Page 157: ...ble connector and insert the connector and cables into the top slot of the front plate Figure 60 Connecting the cable connector and putting into the foam top slot 5 Align the removable front plate into place and push it slightly downward to the bottom Figure 61 Putting front plate into place by slightly pushing downward to the bottom ...

Page 158: ...e and Service Page 158 Integral Fraction Collector FT VF F20 A Operating Manual 6 Tighten the attachment screw to fix the front plate with the screwdriver Figure 62 Tightening two screws and secure the front plate ...

Page 159: ...le wash reservoir rinse the reservoir thoroughly Make sure that no particles dust or algae are present Recommended when using 100 water as needle wash liquid Replace the needle wash liquid daily Use a needle wash liquid that is suitable for your application and that removes residual sample from the needle sufficiently Check the liquid level of the needle wash reservoir Fill the needle wash reservo...

Page 160: ... the cut is at right angle to the length of the line Preparations 1 Prepare fresh needle wash liquid Observe the guidelines for the needle wash liquid in Needle Wash Liquid Guidelines Page 159 2 Empty the needle wash system a Unscrew the cap of the needle wash reservoir b Together with the cap remove the needle wash inlet line from the reservoir c When you remove the needle wash inlet line from th...

Page 161: ...he needle wash pump outlet as shown below then connect the new needle wash outlet line to the free needle wash port inlet and the free needle wash pump outlet and fit in the new needle wash outlet line into the slot on the housing Figure 63 Needle wash outlet line No Description No Description 1 Needle wash port inlet 4 Needle wash port 2 The slot on the housing 5 Needle wash outlet line 3 Needle ...

Page 162: ...flushed continuously until the fresh needle wash liquid is present at the wash port For more details see Important Operating Parameters Page 113 7 7 3 Performing A Needle Wash Cycle The needle wash cycle can be part of the sequence or can be performed manually This section describes how to perform a manual needle wash cycle For information on automatic needle wash cycles using the wash modes in Ch...

Page 163: ... are currently defined in Chromeleon for example between sample sequences perform one of the following options On the keypad select the Wash button In Chromeleon select the External Needle Wash button on the ePanel FC tab After the needle wash cycle has been completed the needle moves up and stays at needle wash port or moves to parking position if the compartment door is open ...

Page 164: ... Recommended when using 100 water as flush solvent replace the flush solvent daily if the flush function is adopted Use a flush solvent that is suitable for your application and that removes residual fraction from flush buffer loop sufficiently Check the liquid level of the flush solvent reservoir Fill the flush solvent reservoir with fresh flush solvent if required If you want to use a different ...

Page 165: ...equired Fresh flush solvent Replacement flush purge lines include following four capillaries Flush solvent tubing from fraction collection valve port 7 to flush solvent reservoir Flush pump to fraction collection valve capillary Flush buffer loop from fraction collection valve port 1 to port 4 Flush waste tubing from fraction collection valve port 5 to Y connector Figure 64 Configuration of flush ...

Page 166: ...s 1 Prepare fresh flush solvent Observe the guidelines for the flush solvent For details see Flush Solvent Guidelines page 164 2 Empty the flush purge system a Unscrew the cap of the flush solvent reservoir b Together with the cap remove the flush solvent tubing from the reservoir c When you remove the flush solvent tubing from the tubing guides be careful not to pull on other tubing in the guides...

Page 167: ...n valve to the flush solvent reservoir in the solvent rack Figure 65 Flush solvent tubing 2 Remove the capillary between flush pump and fraction collection valve port 8 3 Disconnect from the flush pump by unscrewing the fitting 4 Disconnect the Viper fitting from port 8 of fraction collection valve with the Viper knurl Figure 66 Flush pump to fraction collection valve capillary ...

Page 168: ...ollection valve 7 Disconnect the Viper fitting from port 4 of fraction collection valve 8 Then disconnect the Viper fitting from port 5 of fraction collection valve to remove the flush waste tubing Figure 67 Remove flush buffer loop and flush waste tubing TIP If capillaries are to be reinstalled protect the open ends of the capillaries with caps 9 Connect a new flush buffer loop to port 1 and port...

Page 169: ...ubing Remove the cap from the Viper fittings b Connect the Viper fittings to relevant ports of the fraction collection valve and tighten them with the Viper knurled head c Remove the knurls from the Viper fittings due to limited space at the fraction collection valve 10 Connect the capillary between flush pump and port 8 of fraction collection valve Figure 69 Flush purge line connected to fraction...

Page 170: ...llaries by hand For details see Connecting Viper Capillaries page 77 Keep the Viper knurls for reuse in the future 11 Connect a new flush solvent tubing to port 7 of fraction collection valve and route to the flush solvent reservoir in the solvent rack Figure 70 Install the replacement flush solvent tubing a Unpack a replacement flush solvent tubing b Connect the flush solvent tubing onto port 7 o...

Page 171: ...w carry over and high recovery when flush function is enabled The fluidic volume of flush buffer loop shall be around double volume of matched needle capillary 14 Flush the flush pump solvent lines to ensure they are filled with fresh flush solvent see Performing A Flush Purge Cycle Page 171 7 8 3 Performing A Flush Purge Cycle When Routine and preventive maintenance for the flush purge system Rep...

Page 172: ...le capillary No Description No Description 1 Needle capillary Viper fitting on the needle unit 5 S shape slot on the foam part 2 Needle capillary screw 6 Needle capillary press plate 3 Needle capillary support block 7 Needle capillary Viper fitting on the fraction collection valve 4 Needle capillary Tools required Screwdriver Torx T10 Preparations 1 Prepare the device for maintenance move the need...

Page 173: ...it NOTICE The device shall be powered off while replacing the needle capillary Follow these steps 1 Remove the front plate See Removing the Front Plate Page 155 2 Loosen the attachment screw on needle capillary press plate with the screwdriver till the needle capillary can be released from the needle capillary press plate The screw shall stay loose but not removed from the device Figure 72 Looseni...

Page 174: ...n valve with the Viper knurl pre prepared Figure 74 Disconnect Viper Fitting 5 Remove the old needle capillary from the device 6 Unpack the replacement needle capillary carefully Remove the protection cap from the Viper fittings 7 Connect the needle capillary Viper fitting to the port 2 of fraction collection valve Remove the Viper knurl due to limited space at the fraction collection valve and re...

Page 175: ...edle capillary screw Make sure the needle capillary support block is aligned well with the needle unit Figure 76 left correct alignment right wrong alignment 9 Insert the needle capillary into the S shape slot on the foam part and fix the needle capillary with the needle capillary press plate Tighten the attachment screw on the needle capillary press plate to fix the needle capillary press plate a...

Page 176: ...mpartment doors and the device will run initialization automatically Make sure no errors or warnings are reported in the Audit trail 12 In Chromeleon configure the Needle Capillary There are two options of the needle capillary types 0 18 x 415 mm and 0 25 x 415 mm Users can check via the marker on the needle capillary and select accordingly For more details see Setting Up the Device in the Softwar...

Page 177: ... unit into service position See Before Maintenance page 153 2 Turn off the device with its main power switch NOTICE The device shall be powered off while replacing the needle unit Follow these steps 1 Remove the front plate See Removing the Front Plate page 155 2 Remove the needle capillary See Replacing the Needle Capillary page 172 3 Push the lock pin upward so that you can access the attachment...

Page 178: ...n 4 Vial pusher NOTICE Pay attention to the removing and installation process Make sure no extra force or damage to the yellow cable nearby the needle unit 1 Unpack the replacement needle unit and remove the protection parts including a short tubing on the needle tip and a plug 2 Position the needle unit on the installation plate Align the two pins on the rear side of the needle unit into the hole...

Page 179: ...Install the needle unit via two aligning pins No Description No Description 1 Two alignment holes on the front side of the installation plate 2 Two pins on the rear side of the needle unit 3 Tighten the two attachment screws on the needle unit Figure 80 Tighten the needle unit with two attachment screws ...

Page 180: ...and the vial pusher can move up and down easily the needle unit sits correctly in place 5 Reinstall the needle capillary See Replacing the Needle Capillary page 172 6 Install the front plate See Removing the Front Plate page 155 7 Power on the device Close the compartment doors and the device will run initialization automatically Make sure no errors or warnings are reported in the Audit trail ...

Page 181: ...hat the drain pump is turned off Follow these steps Figure 81 Configuration of drain pump head with tubing waste tubing No Description No Description 1 Drain Pump Head 4 Straight Tubing Connector 2 Elbow tubing connector 5 Waste Tubing pre installed with the pump head 3 Condensation Tubing 1 Rotate the drain pump head counterclockwise slightly with and remove it gently 2 Disconnect the condensatio...

Page 182: ...g 5 Mount the replacement drain pump head and rotate it clockwise slightly to the end by hand When you hear a click the replacement drain pump head is successfully installed with pump motor together 6 Insert the right hand side waste tubing of the replacement drain pump with tubing into the relevant hole and secure onto the front leak tray 7 In Chromeleon perform the DrainPumpHeadChanged command 8...

Page 183: ...ure 88 Drain hole on the right side of fraction collection valve Parts and additional items required Replacement fraction collection valve stator Replacement fraction collection valve rotor Preparations Prepare the device for maintenance See Preparing the Device for Maintenance page 153 Make sure to stop pump flow Follow these steps 1 Disconnect all liquid lines connected to the fraction collectio...

Page 184: ...With your fingers or a small tool gently pry the rotor 4 away from the body 5 Examine the rotor sealing surface for scratches If you see any the rotor should be replaced 6 Examine the stator sealing surfaces If scratches are visible between the ports that stator should be replaced 7 Clean all the parts thoroughly with an appropriate solvent taking care that no surfaces get scratched TIP The most c...

Page 185: ...with the two pins on the valve body 10 Insert the five stator screws and tighten them gently until they start to get snug Alternate among the five screws in the sequence as indicated in below figure tightening them in quarter turn 90 increments until the stator is flush against the valve body Figure 90 Loosening and tightening order 11 Reinstall all liquid lines as reversed steps NOTICE The valve ...

Page 186: ...ance procedures When See the steps in the respective maintenance section to obtain if restarting the fraction collector after maintenance is required for the procedure Follow the steps 1 Perform the required parameter updates or adjustments Follow the instructions in the respective maintenance procedures 2 Prepare the fraction collector for operation See Preparing the Device for Operation page 105...

Page 187: ...reparations WARNING Electric Shock High voltages are present inside the device that could cause an electric shock or damage to the device Turn off the device with its main power switch Disconnect the power cord from both the power source and the device Use only the fuses of the type and current rating specified for the device by Thermo Fisher Scientific Do not use repaired fuses and do not short c...

Page 188: ...r to remove the fuse holder To do this insert a small flat screwdriver into the slot next to the fuse holder and remove the cover cap with a lever movement 2 Replace the two fuses with new fuses of the specified type and current rating Always replace both fuses 3 Reinstall the fuse holder 4 Reconnect the power cord to the power source and to the device 5 Turn on the device with the main power swit...

Page 189: ...s released the new version will be included in the next available Chromeleon Service Release The new firmware will not be transferred automatically to the device when you install the Chromeleon Service Release Preparations 1 cacl 2 Connect the device in the Chromeleon software 3 Stop all operations in the Instrument that includes the device 4 Wait until the instrument is idle Follow these steps 1 ...

Page 190: ...hat will be transferred from the Chromeleon software If the firmware installed in the device is a later version than the version in Chromeleon cancel the download The firmware update may take several minutes 3 Monitor the Audit Trail of the Chromeleon Instrument Configuration Manager to see whether the firmware update is successful or fails If the firmware update fails turn the device off and on a...

Page 191: ...or Follow these steps NOTICE To avoid damage to the door hinges be careful when performing the following sequence of steps and do not apply force 1 To remove a door push the door upward while opening Open the door to a position in which the two hinges on the housing are aligned in the grooves on the door You can remove the door only when the hinges are in the grooves Figure 84 Unhinging a door No ...

Page 192: ...oor align the door with the hinges on the housing Be careful not to clamp tubing or capillaries between the door and the enclosure 4 Insert the hinges in the groove by pushing up and slightly turning the door 5 Push the door downward to lock it in place You can close the door only when it is properly installed ...

Page 193: ... lifting or moving requires a team effort of two persons A team effort is in particular required when lifting the device into the system stack or when removing it Use the carrying handles that were shipped with the device to move or transport the device Never move or lift the device by the front doors This will damage the doors or the device Preparing the device for Transport To prepare the device...

Page 194: ...k the leak tray and drain port for liquid If liquid is present absorb the liquid with a tissue 9 On the fraction collection valve disconnect the delay capillary to the detector outlet the flush tubing to the flush solvent reservoir and the needle wash inlet line to the needle wash liquid reservoir 10 Remove the waste line from the Y connector 11 Close open flow connections with appropriate plugs o...

Page 195: ...ing manual b To prepare the device for first time operation follow the instructions in the Preparing the device for Operation section in this operating manual 5 Before starting an analysis let the device equilibrate and be sure that it is ready for operation Shipping the device To ship the device follow these steps 1 Follow the unpacking instructions in this manual in the reverse order Use only th...

Page 196: ... accept devices for repair if the Decontamination Certificate is missing Restarting the device after Shipping To restart the device after shipping follow these steps 1 Follow the unpacking instruction in this operating manual 2 Install and set up the device in the system stack Follow the instructions on mounting the system stack in the Vanquish System Operating Manual 3 Set up the device a To conn...

Page 197: ...e device including lifting or moving requires a team effort of two persons A team effort is in particular required when lifting the device into the system stack or when removing it 7 18 1 Removing the Slide In Module Tools required Screwdriver Torx T20 Preparations Prepare the device for transport See the Transporting or Shipping the device section in this operating manual Follow these steps 1 Loo...

Page 198: ... NOTICE The slide in module can fall down when pulling it out of the enclosure too far Pull out the slide in module just far enough so that you can grasp it on both sides from below 4 Remove the slide in module from the enclosure The following steps require a team effort a Take the slide in module on both sides from below b Pull the slide in module from the rails towards the front c Place the slid...

Page 199: ...ill in and sign the Decontamination Certificate which is part of the Service Return Form Sign the certificate to confirm that the device has been properly decontaminated and that it is free of hazardous substances Thermo Fisher Scientific refuses to accept devices for repair if the Decontamination Certificate is missing 7 18 2 Installing the Slide In Module Parts required Replacement slide in modu...

Page 200: ...he slide in module on both sides from below b Lift the slide in module to the height of the enclosure c Place the slide in module in the enclosure so that the module sits in the enclosure by approximately 25 cm d Push the slide in module onto the rails and into the enclosure until the slide in module sits completely in the enclosure Figure 87 Inserting the slide in module 4 Gradually and evenly ti...

Page 201: ...hapter in this operating manual b To prepare the slide in module for first time operation follow the instructions in the Preparing the device for Operation section in this operating manual 6 Prepare all other modules of the Vanquish system for operation and restart them Refer to the Operating Manuals for the modules 7 Before starting an analysis let the chromatography system equilibrate and be sur...

Page 202: ...8 Troubleshooting Page 202 Integral Fraction Collector FT VF F20 A Operating Manual 8 Troubleshooting This chapter is a guide to troubleshooting issues that may arise during operation of the device ...

Page 203: ...ators The status indicator LED bar on the front side of the device and the STATUS LED on the keypad inside provide quick visual feedback on the operational status of the device If the device firmware detects a problem the status indicators are red The problem is reported to the Chromeleon software and a message appears in the Audit Trail Alarms Leaks are a potential safety issue Therefore if a lea...

Page 204: ...orrect the problem An error does not interrupt the current analysis However if the error occurs during the Ready Check the analysis will not be started or Abort The queue cannot be started A running queue or batch is stopped immediately The issue must be resolved first For possible causes and recommended remedial actions see Messages page 205 Firmware Failure If a firmware failure occurred during ...

Page 205: ...al Action Unexpected module behavior Code XX XX Two digit to four digit code number When the message appears write down the message code and turn off the module Wait for 5 seconds and turn on the module again TIP If the message appears again contact Technical Support Module malfunction detected Code XX XX Two digit to four digit code number When the message appears write down the message code Turn...

Page 206: ...ent the warning message will be shown in the user level audit trail and drain pump is triggered to drain out liquid to waste drain port The device keeps monitoring the status of the rear leak sensor and if the sensor gets dry the drain pump will stop working immediately Possible causes triggering the warning as follows 1 Accumulated condensation if the device remains running at cooling mode for a ...

Page 207: ...010 Vial not found Check for correct vial position in the rack and sample sequence Vial not found with InVial collection mode Check if vials or well plate are present at the specified positions Check the Frac Start position start collection position in FC ePanel tab and then check the settings in the sequence view where individual collection position is specified Specify a different tube position ...

Page 208: ...issue persists power cycle the device and check again if it is recovered If the issue still persists contact Technical Support Code 9021 Temperature sensor open cabin air Power cycle the device If the issue persists contact Technical Support Code 9022 Temperature sensor short cabin air Power cycle the device If the issue persists contact Technical Support Code 9023 Temperature sensor open ambient ...

Page 209: ...ter at 1 2mL min for another 10mins Make sure both collect position and waste position are involved The purpose is to eliminate air bubbles inside the flow Then re do the DVD function and check if it can be implemented successfully If the issue persists contact Technical Support Code 9038 Air bubble sensor abnormal DVD cannot work Air bubble sensor is not detected by system Turn the device off wai...

Page 210: ...osition button on ePanel FC tab Check the racks and make sure the Tube Position and Move Needle Position are in correct format and in range Code 9054 Air bubble sensor abnormal during power up check Power cycle the device If the issue persists contact Technical Support Code 9055 Internal needle rinse failed Pump speed is 0 Flow rate of upstream Vanquish pump is 0 This may occur when performing Int...

Page 211: ... off with Tube Wrapping disabled in the instrument method This warning indicates that the current collection has finished successfully but the last available tube has been used Enable Tube Wrapping or replace the racks and reset the Tube Position on ePanel FC tab otherwise users will not be able to start another collection An error Maximum number of installed tubes used up will be popped up during...

Page 212: ...fety guidelines and general rules for maintenance and service as presented in Maintenance and Service page 142 Follow these steps 1 Locate the source of the leak As leakage usually occurs at a connection visually inspect all components and connections in the flow path 2 Tighten or replace the connection or component as required 3 With a cloth or tissue thoroughly absorb all liquid that has collect...

Page 213: ... needle unit 3 Place the open connector of the needle capillary into the central hole of needle wash port 4 Check that the fraction collection valve is set to collection position 5 In Chromeleon set the pump flow rate to the maximum pump flow rate 6 Turn on the pump flow and flush the needle capillary to wash port at the maximum pump flow rate for approximately 1 minute Afterward turn off the pump...

Page 214: ...llection valve to waste position flush the waste line at the maximum pump flow rate for approximately 1 minute Afterward turn off the pump flow 12 Restart the device with its main power switch The device performs a self test The needle initializes and adjusts its position 13 Afterward turn off the pump flow 8 3 3 Temperature Setpoint Cannot Be Achieved Causes The ambient temperatures may be out of...

Page 215: ...8 Troubleshooting Integral Fraction Collector FT VF F20 A Operating Manual Page 215 ...

Page 216: ... 216 Integral Fraction Collector FT VF F20 A Operating Manual 9 Specifications This chapter provides the physical and performance specifications including information about the materials used in the flow path of the device ...

Page 217: ... with mobile phase into needle wash port before or after each injection Dip wash and continuous wash of outer needle surface Flush function between fractions to flush inner needle surface and needle capillary with flush liquid into sample container Operating Flow Range 0 05 mL min 10 mL min Delay Volume 19 µL with Fraction collector inlet to fraction collection valve port 2 7 µL default time based...

Page 218: ...l Standard 11 µL 10mm P N 6077 0250 Flow cell Semi micro 2 5 µL 7mm P N 6077 0360 Flow cell Standard 13 µL 10mm P N 6083 0510 Flow cell Semi Analyt 5 µL 7mm P N 6083 0520 Flow cell Semi micro 2 5 µL 7mm P N 6083 0530 Flow cell Semi micro Bio 2 5 µL 7mm P N 6083 0550 LightPipe flow cell Standard 2 µL 10 mm P N 6083 0100B LightPipe flow cell High Sensitivity 13 µL 60 mm P N 6083 0200B Primary Wetted...

Page 219: ...tion Safety features Leak detection and safe leak handling Good Laboratory Practice GLP features Predictive Performance functions for scheduling maintenance procedures based on the actual operating and usage conditions of the device All system parameters are logged in the Chromeleon CDS Audit Trail ...

Page 220: ...ge Temperature 20 C 45 C Ambient Operating Humidity 20 80 RH non condensing Ambient Storage Humidity Maximum 60 RH non condensing Operating Altitude Maximum 2000 m above sea level Pollution degree 2 Overvoltage category II Power requirements 100 240 VAC 10 50 60 Hz max 525 W 550 VA Emission sound pressure level 70 dB A typically 50 dB A Dimensions height x width x depth 23 x 42 x 62 cm 9 1 x 16 5 ...

Page 221: ...9 Specifications Integral Fraction Collector FT VF F20 A Operating Manual Page 221 ...

Page 222: ... F20 A Operating Manual 10 Accessories Consumables and Replacement Parts This chapter describes the standard accessories that are shipped with the device and the accessories that are available as an option This chapter also provides information for reordering consumables and replacement parts ...

Page 223: ... with the replacement parts and additional components options and peripherals specifically authorized and qualified by Thermo Fisher Scientific Accessories consumables and replacement parts are always maintained at the latest technical standard Therefore part numbers are subject to change If not otherwise stated updated parts will be compatible with the parts they replace ...

Page 224: ...0 mm MP35N 1 Delay capillary connecting detector and fraction collection valve for time based fraction collection for flow rates up to 2 mL min in two stacks Viper ID x L 0 10 x 550 mm MP35N 1 Delay capillary connecting detector and fraction collection valve for time based fraction collection from 2 10 mL min in two stacks Viper ID x L 0 18 x 550 mm MP35N 1 Flush Solvent Tubing ID x L 8 mm x 1 5 m...

Page 225: ...ting Manual Page 225 Item Quantity in shipment System Interlink Cable RJ45 91cm 1 Tubing bracket 1 USB 2 0 cable type A to type B High speed USB 2 0 1m 1 Vial septum kit including vials and caps with pre assembled septa 1 For reordering information see Consumables and Replacement Parts page 228 ...

Page 226: ...5 0 5 mL min One stack or two stacks Delay capillary peak based FC up to 0 5 mL min Viper ID x L 0 18 x 1200 mm MP35N 6706 1100 0 5 1 mL min Delay capillary peak based FC 0 5 1 mL min Viper ID x L 0 25 x 1500 mm MP35N 6706 1110 1 2 mL min Delay capillary peak based FC 1 2 mL min Viper ID x L 0 5 x 800 mm PEEK 6706 1120 2 10 mL min Delay capillary peak based FC 2 10 mL min ID x L 1 x 1000 mm OD 1 1...

Page 227: ...F F20 A Operating Manual Page 227 Needle capillaries Item Part No Remarks Needle Capillary ID 0 18 mm MP35N with partial PEEK shielding 6706 1010 For flow rates up to 5 mL min Needle Capillary ID 0 25 mm MP35N with partial PEEK shielding 6706 1020 For flow rates between 5 10 mL min ...

Page 228: ...p port Flush Solvent Tubing ID x L 8 mm x 1 5 m OD 1 16 FEP 6706 1700 Needle Wash Port Tubing ID x L 1 0 x 130 mm OD 1 16 PEEK 6706 1140 Waste tubing to Y connector Viper PEEK 6706 1060 fraction collection valve port 5 to Y connector For other system capillaries refer to the Vanquish System Operating Manual Flow components Description Part No Needle unit ID 0 3mm MP35N 6706 1000 Rotor fraction col...

Page 229: ... Power cord Denmark 6000 1070 Power cord EU 6000 1000 Power cord India SA 6000 1090 Power cord Italy 6000 1040 Power cord Japan 6000 1050 Power cord UK 6000 1020 Power cord USA 6000 1001 Power cord Switzerland 6000 1030 Reservoirs for solvents and wash liquids Description Part No Bottlecap Retaining Guide 1 16 6261 0216 Cap for reservoirs screw cap pack of 4 6270 0013 Cap plug to close open holes ...

Page 230: ...for Vanquish rack type identification 6850 1026 Sample rack for 96 x 7 mm O D vials With 2D barcode for Vanquish rack type identification 6850 1030 Sample rack for 96 x 8 mm O D vials With 2D barcode for Vanquish rack type identification 6850 1034 For well plates refer to the ordering information that is included in the ship kit of the Vanquish Split Sampler Tubing and wash components Description ...

Page 231: ...10 Accessories Consumables and Replacement Parts Integral Fraction Collector FT VF F20 A Operating Manual Page 231 ...

Page 232: ...11 Appendix Page 232 Integral Fraction Collector FT VF F20 A Operating Manual 11 Appendix This chapter provides additional information about compliance ...

Page 233: ...Vus Compliance The cTUVus label on the device indicates that the device has satisfied the requirements for the cTUVus mark Compliance with the applicable standards has been evaluated by TÜV Rheinland Shanghai Co Ltd RoHS Compliance This product complies with the RoHS Restrictions of Hazardous Substances directives European RoHS Directive Directive on the restriction of the use of certain hazardous...

Page 234: ...when used as intended will not cause serious damage to human health or environment For more information go to http www thermofisher com us en home technical resources rohs certificates html 11 1 2 WEEE Compliance This product is required to comply with the European Union s Waste Electrical Electronic Equipment WEEE Directive It is marked with the following symbol Figure 88 WEEE symbol Thermo Fishe...

Page 235: ...e reasonable protection against harmful interference when the equipment is operated in a commercial environment This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instructions may cause harmful interference to radio communications Operation of this equipment in a residential area is likely to cause harmful interference in which...

Page 236: ...12 Index Page 236 Integral Fraction Collector FT VF F20 A Operating Manual 12 Index ...

Page 237: ...ator 126 127 Delay volume determination Autosampler types enabling DVD 125 Delay volume determination DVD 118 120 Calculator 126 127 Detector types disabling DVD 126 Detector types enabling DVD 125 Delay Volume Determination DVD Disabled 127 Delay volume determination DVD Flow cells enabling DVD 126 Modules 125 Pump types enabling DVD 125 Digital I O 65 Door After power off 146 Opening mechanism 6...

Page 238: ...6 InVial 117 132 Positioning mode 116 Puncture offset 117 Rinse internal 114 Rinse mode 115 Wash external 114 Wash Liquid 81 Wash mode 115 Wash System 80 Needle height 111 Needle pusher positions AboveVial 133 InVial 135 Needle wash Liquid guidelines 159 Install 81 Maintenance 159 Purge 114 Replace wash lines 160 Reservoir 81 Wash cycle 162 O Operating parameters 113 Operating principle 34 Operati...

Page 239: ...g term 139 Short term 138 Signal words 19 Slide in module 197 Install 199 Remove 197 Smart Shutdown 138 Smart Standby 138 Smart Startup 107 Solvent Information 28 Specifications Performance 217 Physical 220 Startup 106 Status indicator LED bar 100 102 203 Status LED 100 102 203 Status LED 100 102 203 System equilibration 106 System interlink 65 System power button 104 T Temperature control 113 Tem...

Page 240: ...Thermo Fisher Scientific Inc 168 Third Avenue Waltham Massachusetts 02451 USA www thermofisher com ...

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