Troubleshooting
Page 44 of 60
M1393R0
OQ/PQ Operating Instructions
5.3
Probable Causes and Solutions
Test Procedure
Problem
Probable Cause
Solution
All
No enough COM ports
available.
•
-
•
Control Switchos
valves by event
outputs.
Lamp Intensity of the UV
Detector
Intensity values too
low.
•
Old lamp.
•
Wrong wavelength.
•
Integration time too
low.
•
Replace.
•
Check/change.
•
Check/adjust.
Failed.
•
Filter not in the
optical path.
•
Check filter
position.
Intensity values too
low.
•
•
Dirty flow cell
•
•
Clean/replace.
Flow rate too low.
•
Solvent filter
clogged.
•
Check/replace.
Drift too high.
•
Old lamp.
•
Replace.
Temperature readout
not within the
specifications.
•
Temperature probe
not properly
installed.
•
Install properly (e.g.
use a 4 mm screw).
Signal is out of range.
•
Acetone
concentration too
high.
•
Dilute with mobile
phase A.
‘Ghost” peak before
the caffeine peak.
•
Contaminated
injection valve
•
Extend wash step.
•
Sonicate rotor and
stator in CAN for
10 min.
Caffeine peak shows
some ‘spikes’,
improper integration.
•
Air in the syringe.
•
Check syringe
speed and time
Reproducibility of the
Injection Volume
Linearity of the injection
(4.9)
•
Air in the syringe.
•
Air at the bottom of
the sample vial.
•
Purge syringe,
•
Remove air.
Considerable drift of
the 95% to 100% B
steps.
•
No/too little formic
acid in mobile
phase A and B.
•
Check/replace
solvents.
Result not within
specification
•
Wrong delay time.
•
Hardware.
•
Check/modify.
•
Check filter, valves,
etc.
Flow rate too low.
•
•
Switchos Flow Rate and
Pressure Stability Test
Pulsation too high.
•
Check valve not
working properly.
•
Clean/replace.
Both position LEDs are
illuminated at the
same time.
•
Improper
initialization or
faulty controller.
•
See Service
Information #043.