Thermo Scientific TX-750 Instruction Manual Download Page 1

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50119938-4

October 2009

Thermo Fisher Scientific

TX-750

Instruction Manual

Summary of Contents for TX-750

Page 1: ...50119938 4 October 2009 Thermo Fisher Scientific TX 750 Instruction Manual ...

Page 2: ...ment is for reference purposes only System configurations and specifications in this document supersede all previous information received by the purchaser Thermo Fisher Scientific Inc makes no representations that this document is complete accurate or error free and assumes no responsibility and will not be liable for any errors omissions damage or loss that might result from any use of this docum...

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Page 5: ...et 75003614 1 9 Sorvall 100V 50 60Hz Round bucket 75003608 1 10 Sorvall 100V 50 60Hz rectangular bucket 75003614 1 11 Thermo Scientific 1 12 Thermo Scientific 230V 50Hz 60Hz Round bucket 75003608 1 12 Thermo Scientific 230V 50Hz 60Hz rectangular bucket 75003614 1 13 Thermo Scientific 120V 60Hz Round bucket 75003608 1 14 Thermo Scientific 120V 60Hz rectangular bucket 75003614 1 15 Chapter 2 Accesso...

Page 6: ...ntervalls 6 2 Cleaning 6 2 Disinfection 6 3 Decontamination 6 4 Autoclaving 6 5 Service of Thermo Fisher Scientific 6 6 RCF Values A 1 Rectangular buckets 75003614 A 1 Round bucket 75003608 A 2 Chemical Compatibility Chart B 1 Warranty and recommended Service Life C 1 Contact Information D 1 ...

Page 7: ...ty Scope of Supply If any parts are missing please contact the nearest Thermo Fisher Scientific representative Precautions In order to ensure safe operation of the TX 750 the following general safety regulations must be followed Do not remove the magnet at the rotor bottom Do not use rotors which show any signs of corrosion and or cracks Do not touch the electronic components of the rotor and do n...

Page 8: ...particular attention to the following aspects Rotor installation Check that the rotor is locked properly into place before operating the centrifuge Always balance the samples Maximum sample density at maximum speed 1 2 g cm 3 This symbol refers to general hazards ATTENTION means that material damage could occur WARNING means that injuries or material damage or contamination could occur This symbol...

Page 9: ...Thermo Scientific 75003607 TX 750 1 1 1 Rotor Specifications Contents Heraeus on page 1 2 Sorvall on page 1 6 Thermo Scientific on page 1 12 ...

Page 10: ...5 55 sample heating at nmax C referred to ambient temperature of 23 C running time 120 minutes 11 11 11 aerosol tight Tested by HPA Porton down UK yes yes yes permissible temperature range autoclavable C 121 121 121 Centrifuge Heraeus Multifuge X3R Heraeus Multifuge X3 FR Heraeus Megafuge 40R Catalog 75004515 75004536 75004518 Weight empty kg 7 4 7 4 7 4 max cycle number 50 000 50 000 50 000 Maxim...

Page 11: ...45 50 sample heating at nmax C referred to ambient temperature of 23 C running time 120 minutes 11 11 11 aerosol tight Tested by HPA Porton down UK yes yes yes permissible temperature range autoclavable C 121 121 121 Centrifuge Heraeus Multifuge X3R Heraeus Multifuge X3 FR Heraeus Megafuge 40R Catalog 75004515 75004536 75004518 Weight empty kg 6 8 6 8 6 8 max cycle number 50 000 50 000 50 000 Maxi...

Page 12: ...mple heating at nmax C referred to ambient temperature of 23 C running time 120 minutes 11 11 11 aerosol tight yes yes yes permissible temperature range autoclavable C 121 121 121 Tested by HPA Porton down UK Centrifuge Heraeus Multifuge X3R Heraeus Multifuge X3 FR Heraeus Megafuge 40R Catalog 75004516 75004537 75004519 Weight empty kg 7 4 7 4 7 4 max cycle number 50 000 50 000 50 000 Maximum perm...

Page 13: ...0 sample heating at nmax C referred to ambient temperature of 23 C running time 120 minutes 8 8 8 aerosol tight Tested by HPA Porton down UK yes yes yes permissible temperature range autoclavable C 121 121 121 Centrifuge Heraeus Multifuge X3R Heraeus Multifuge X3 FR Heraeus Megafuge 40R Catalog 75004516 75004537 75004519 Weight empty kg 6 8 6 8 6 8 max cycle number 50 000 50 000 50 000 Maximum per...

Page 14: ... 55 sample heating at nmax C referred to ambient temperature of 23 C running time 120 minutes 11 11 11 aerosol tight Tested by HPA Porton down UK yes yes yes permissible temperature range autoclavable C 121 121 121 Centrifuge Sorvall Legend XTR Sorvall Legend XFR Sorvall ST 40R Catalog 75004520 75004538 75004524 Weight empty kg 7 4 7 4 7 4 max cycle number 50 000 50 000 50 000 Maximum permissible ...

Page 15: ...50 sample heating at nmax C referred to ambient temperature of 23 C running time 120 minutes 11 11 11 aerosol tight Tested by HPA Porton down UK yes yes yes permissible temperature range autoclavable C 121 121 121 Centrifuge Sorvall Legend XTR Sorvall Legend XFR Sorvall ST 40R Catalog 75004520 75004538 75004524 Weight empty kg 6 8 6 8 6 8 max cycle number 50 000 50 000 50 000 Maximum permissible l...

Page 16: ...mple heating at nmax C referred to ambient temperature of 23 C running time 120 minutes 11 11 11 aerosol tight Tested by HPA Porton down UK yes yes yes permissible temperature range autoclavable C 121 121 121 Centrifuge Sorvall Legend XTR Sorvall Legend XFR Sorvall ST 40R Catalog 75004521 75004539 75004525 Weight empty kg 7 4 7 4 7 4 max cycle number 50 000 50 000 50 000 Maximum permissible load g...

Page 17: ...0 sample heating at nmax C referred to ambient temperature of 23 C running time 120 minutes 8 8 8 aerosol tight Tested by HPA Porton down UK yes yes yes permissible temperature range autoclavable C 121 121 121 Centrifuge Sorvall Legend XTR Sorvall Legend XFR Sorvall ST 40R Catalog 75004521 75004539 75004525 Weight empty kg 6 8 6 8 6 8 max cycle number 50 000 50 000 50 000 Maximum permissible load ...

Page 18: ...sample heating at nmax C referred to ambient temperature of 23 C running time 120 minutes 10 10 10 aerosol tight Tested by HPA Porton down UK yes yes yes permissible temperature range autoclavable C 121 121 121 Centrifuge Sorvall Legend XTR Sorvall Legend XFR Sorvall ST 40R Catalog 75004522 75004540 75004526 Weight empty kg 7 4 7 4 7 4 max cycle number 50 000 50 000 50 000 Maximum permissible load...

Page 19: ...5 50 sample heating at nmax C referred to ambient temperature of 23 C running time 120 minutes 8 8 8 aerosol tight Tested by HPA Porton down UK yes yes yes permissible temperature range autoclavable C 121 121 121 Centrifuge Sorvall Legend XTR Sorvall Legend XFR Sorvall ST 40R Catalog 75004522 75004540 75004526 Weight empty kg 6 8 6 8 6 8 max cycle number 50 000 50 000 50 000 Maximum permissible lo...

Page 20: ...el braking time s 55 55 55 55 sample heating at nmax C referred to ambient temperature of 23 C running time 120 minutes 11 11 aerosol tight Tested by HPA Porton down UK yes yes permissible temperature range autoclavable C 121 121 Centrifuge Thermo Scientific SL 40R Thermo Scientific SL 40 FR Catalog 75004527 75004543 Weight empty kg 7 4 7 4 max cycle number 50 000 50 000 Maximum permissible load g...

Page 21: ...king time s 45 50 45 50 sample heating at nmax C referred to ambient temperature of 23 C running time 120 minutes 11 11 aerosol tight Tested by HPA Porton down UK yes yes permissible temperature range autoclavable C 121 121 Centrifuge Thermo Scientific SL 40R Thermo Scientific SL 40 FR Catalog 75004527 75004543 Weight empty kg 6 8 6 8 max cycle number 50 000 50 000 Maximum permissible load g 4x750...

Page 22: ...0 Accel braking time s 90 60 sample heating at nmax C referred to ambient temperature of 23 C running time 120 minutes 11 aerosol tight Tested by HPA Porton down UK yes permissible temperature range autoclavable C 121 Centrifuge Thermo Scientific SL 40R Catalog 75004528 Weight empty kg 7 4 max cycle number 50 000 Maximum permissible load g 4x800 maximum speed nmax rpm 4 700 maximum RCF value at nm...

Page 23: ...e 90 Accel braking time s 70 50 sample heating at nmax C referred to ambient temperature of 23 C running time 120 minutes 8 aerosol tight Tested by HPA Porton down UK yes permissible temperature range autoclavable C 121 Centrifuge Thermo Scientific SL 40R Catalog 75004528 Weight empty kg 6 8 max cycle number 50 000 Maximum permissible load g 4x750 maximum speed nmax rpm 4 300 maximum RCF value at ...

Page 24: ...Thermo Scientific 75003607 TX 750 2 1 2 Accessories Contents Rotor data on page 2 2 Accessories for round bucket on page 2 2 Rotor data on page 2 4 Accessories for rectangular bucket on page 2 4 ...

Page 25: ...c Conical 4 62 5x139 Included 4 PP Sealing 75003710 377585 4 1 3143 0175 175 175 PP Nalgene Conical 4 62 5x139 Included 4 PP Sealing 75003710 DS3126 0175 4 1 3144 0175 175 175 PC Nalgene Conical 4 62 5x139 Included 4 PP Sealing 75003710 DS3126 0175 4 1 76009007 100 75 Glass Bottle 10 4 000 45x123 75003713 4 1 76009084 100 75 PP Bottle 1 4 700 45x98 75003713 4 1 76009095 100 75 PC Bottle 1 4 700 45...

Page 26: ... 18x124 75003718 4 12 3139 0010 10 8 PP Oak Ridge Tube 10 4 700 16x82 Included 10 PP Sealing 75003719 4 21 3138 0010 10 8 PC Oak Ridge Tube 10 4 700 16x82 Included 10 PP Sealing 75003719 4 21 10 Blood Collection 16x100 75003719 4 21 7 Blood Collection 13x100 75003723 4 27 5 Blood Collection 13x75 75003723 4 27 5 RIA Tubes 13x75 75003724 4 37 5 RIA Decanting Tubes 13x75 75003732 4 37 1 5 2 Conical ...

Page 27: ...ss Bottle 10 4 700 44x100 75003742 4 1 76009084 100 75 PP Bottle 1 4 700 45x98 75003742 4 1 76009095 100 75 PC Bottle 1 4 700 45x98 75003742 4 1 334959 50 50 Nunc Conical 25 4 700 30x121 Included 25 PP Sealing 75003685 4 5 50 Round Bottom Open Topped Tube 35x121 75003749 4 3 3139 0050 50 50 PP Oak Ridge Tube 10 4 700 29x114 Included 10 PP Sealing 75003750 4 4 3138 0050 50 50 PC Oak Ridge Tube 10 4...

Page 28: ...58 4 9 3139 0010 10 8 PP Oak Ridge Tube 10 16x82 Included 10 PP Sealing 75003767 4 16 3138 0010 10 8 PC Oak Ridge Tube 10 16x82 Included 10 PP Sealing 75003767 4 16 10 Blood Collection 16x100 75003767 4 16 7 Blood Collection 13x100 75003768 4 20 5 Blood Collection 13x75 75003768 4 20 5 RIA Tubes 13x75 75003769 4 28 1 5 2 Conical Microtube 11x50 75003770 4 56 MP Standard Microplates up to 20mm 7500...

Page 29: ...Thermo Scientific 75003607 TX 750 3 1 3 AutoLockTM Contents Rotor Installation on page 3 2 Removing the Rotor on page 3 3 ...

Page 30: ...ge spindle The rotor clicks automatically into place 3 Check if the rotor is properly installed by lifting slightly on the handle If the rotor can be pulled up then it must be reclamped to the centrifuge spindle CAUTION Unapproved or incorrectly combined accessories can cause serious damage to the centrifuge CAUTION Do not force the rotor onto the centrifuge spindle If the rotor is very light then...

Page 31: ... and press against the green AutoLockTM button At the same time pull the rotor directly upwards with both hands and remove it from the centrifuge spindle Make sure not to jam the rotor while doing this Figure 4 Handling of AutoLockTM CAUTION Check that the rotor is properly locked on the centrifuge spindle before each use by pulling it a its handle Be sure to check all seals before starting any ae...

Page 32: ...Thermo Scientific 75003607 TX 750 4 1 4 Rotor Loading Contents Before a Run on page 4 2 Proper Loading on page 4 2 Improper Loading on page 4 3 Maximum Loading on page 4 3 Cycle Counter on page 4 4 ...

Page 33: ...t caps 6 Check the rotor bolts and apply grease 7500 3786 before using for the first time 7 Check that each bucket or microplate carrier can swing freely by moving it carefully with your hand Weigh the bucket content adaptor and tube Make sure you do not exceed the maximum compartment load Proper Loading To ensure safe operation of the centrifuge the rotor must be evenly loaded at all times You ca...

Page 34: ...n when spinning at top speed The safety system of the centrifuge requires that you do not overload the rotor There are two options available for centrifuging samples whose weight including adaptor exceeds the maximum permissible load Reduce the fill level Reduce the speed Use the table or the formula nadm admissible speed nmax maximum speed nadm nmax Maximum permissible load Effective load ...

Page 35: ... Load g cavity RPM max Actual Load g cavity RPM max 800 4700 750 4500 820 4642 770 4441 840 4587 790 4385 860 4533 810 4330 880 4481 830 4278 900 4431 850 4227 920 4383 870 4178 940 4336 890 4131 960 4290 910 4085 980 4246 930 4041 1000 4204 950 3998 1020 4162 970 3957 1040 4122 990 3917 1060 4083 1010 3878 1080 4045 1030 3840 1100 4008 1050 3803 WARNING Replace the rotor when the specified number...

Page 36: ...Thermo Scientific 75003607 TX 750 5 1 5 Aerosol tight Applications Contents Basic Principles on page 5 2 Fill level on page 5 3 Checking the aerosol Tightness on page 5 2 ...

Page 37: ...osol tight applications are not possible if the bucket tops are open Aerosol tightness requires the correct operation when filling the sample vessels and closing the rotor lid Quick test As a quick test it is possible to test the aerosol tight beaker and fixed angle rotors using the following process 1 Lubricate all seals lightly Always use the special grease 7600 3500 when lubricating the seals 2...

Page 38: ...he latch clicks into place Figure 6 Bucket with closed lid CAUTION Prior to each use the seals in the rotor are to be inspected in order to assure that they are correctly seated and are not worn or damaged Damaged seals are to be replaced immediately Replacement seals are supplied with the rotors and can also be re ordered as a spare parts set See table in chapter 2 When loading the rotor ensure t...

Page 39: ...607 TX 750 6 1 6 Maintenance and Care Contents Cleaning Intervalls on page 6 2 Cleaning on page 6 2 Disinfection on page 6 3 Decontamination on page 6 4 Autoclaving on page 6 5 Service of Thermo Fisher Scientific on page 6 6 ...

Page 40: ...e parts Use only disinfectants with a pH of 6 8 Dry aluminum parts off with a soft cloth After cleaning treat the entire surface of aluminum parts with corrosion protection oil 7000 9824 Also treat the cavities with oil Store the aluminum parts at room temperature or in a cold storage room with the cavities pointing down Maintenance Recommended Interval Clean rotor chamber daily or when polluted C...

Page 41: ...material has spilled during centrifugation Use a sprayer whenever possible so that all surfaces are covered evenly The rotor chamber and the rotor should be treated preferably with a neutral disinfectant A disinfectant spray would be most suitable for this purpose so that the rotor and accessory surfaces are covered evenly CAUTION When cleaning do not allow liquids especially organic solvents to g...

Page 42: ...n a warm air cabinet at a maximum temperature of 50 C After cleaning treat the entire surface of aluminum parts with corrosion protection oil 7000 9824 Also treat the cavities with oil Tread the bold of the swing out rotor with bold grease 75003786 Decontamination Decantaminate the centrifuge immediately whenever radioactive material has spilled during centrifugation For general radioactive decont...

Page 43: ...with a cloth or in a warm air cabinet at a maximum temperature of 50 C After cleaning treat the entire surface of aluminum parts with corrosion protection oil 7000 9824 Also treat the cavities with oil Tread the bold of the swing out rotor with bold grease 75003786 Autoclaving 1 Before autoclaving clean rotor and accessories and described above 2 Place the rotor on a flat surface Rotors and adapte...

Page 44: ... having the centrifuge and accessories serviced once a year by an authorized service technician The service technicians check the following the electrical equipment the suitability of the set up site the lid lock and the safety system the rotor the fixation of the rotor and the drive shaft Thermo Fisher Scientific offers inspection and service contracts for this work ...

Page 45: ...5 81 177 1000 8 9 19 5 100 218 1100 8 9 19 5 120 264 1200 8 9 19 5 143 314 1300 8 9 19 5 168 368 1400 8 9 19 5 195 427 1500 8 9 19 5 224 491 1600 8 9 19 5 255 558 1700 8 9 19 5 288 630 1800 8 9 19 5 322 706 1900 8 9 19 5 359 787 2000 8 9 19 5 398 872 2100 8 9 19 5 439 961 2200 8 9 19 5 482 1055 2300 8 9 19 5 526 1153 2400 8 9 19 5 573 1256 2500 8 9 19 5 622 1363 2600 8 9 19 5 673 1474 2700 8 9 19 ...

Page 46: ...4300 8 9 19 5 1840 4031 4400 8 9 19 5 1926 4221 4500 8 9 19 5 2015 4415 Table A 2 Round bucket 75003608 Speed rpm Min radius Max radius RCF Rmin RCF Rmax 300 8 3 19 5 8 20 400 8 3 19 5 15 35 500 8 3 19 5 23 55 600 8 3 19 5 33 78 700 8 3 19 5 45 107 800 8 3 19 5 59 140 900 8 3 19 5 75 177 1000 8 3 19 5 93 218 1100 8 3 19 5 112 264 1200 8 3 19 5 134 314 1300 8 3 19 5 157 368 1400 8 3 19 5 182 427 15...

Page 47: ... 8 3 19 5 835 1962 3100 8 3 19 5 892 2095 3200 8 3 19 5 950 2232 3300 8 3 19 5 1011 2374 3400 8 3 19 5 1073 2520 3500 8 3 19 5 1137 2671 3600 8 3 19 5 1203 2825 3700 8 3 19 5 1270 2985 3800 8 3 19 5 1340 3148 3900 8 3 19 5 1411 3316 4000 8 3 19 5 1485 3488 4100 8 3 19 5 1560 3665 4200 8 3 19 5 1637 3846 4300 8 3 19 5 1716 4031 4400 8 3 19 5 1796 4221 4500 8 3 19 5 1879 4415 4600 8 3 19 5 1964 4613...

Page 48: ... S U S S S S M S S S S S S S S S M U U S S Formic Acid 100 S M U U U S M U U S S U S U S U Ammonium Acetate S S U S S S S S S S S S S U S S S S S S S S S S Ammonium Carbonate M S U S S S S S S S S S S S U U S S S S S S M S S S Ammonium Hydroxide 10 U U S U S S M S S S S S S U M S S S S S S S S S M S Ammonium Hydroxide 28 U U S U S U M S S S S S U S U M S S S S S S S S S M S Ammonium Hydroxide conc...

Page 49: ...U S U S U S S S M S S S S S S Dimethylsulfoxide S S U U S S S S U S S U S U U S S U U S S S S U U Dioxane M S U U S S M M S U U S U M U U M M M U S S S S U U Ferric Chloride U U S M S M S S S S M U S S Acetic Acid Glacial S S U U S S U M S U S U U U U U M S U M U S U U S U Acetic Acid 5 S S M S S S M S S S S S M S S S S S S S M S S M S S M Acetic Acid 60 S S U U S S U S M S U U M U S M S M S M S M...

Page 50: ...roxide conc U U M U M M S S U M U U U S M M U U U U Potassium Permanganate S S S S S S S S S U S S S M S M S U S S M S U S Calcium Chloride M U S S S S S S S S S S S S M S S S S S S S M S S S Calcium Hypochlorite M U S M M S M S S M S S S S M S M U S S Kerosene S S S S S S U S M U S U M M S M M M S S U S S U S Sodium Chloride 10 S S S S S S S S S S S S S S S S S S M S Sodium Chloride sat d U S U S...

Page 51: ...S S S S M S S S S S S M S S S S S S S S S S Nickel Salts U S S S S S S S S S S S S S S S S S S M S S S Oils Petroleum S S S S U S S S S U U M S M U U S S S U S S S S Oils Other S S S M S S S S U S S S S U S S S S S S M S Oleic Acid S U S S S U U S U S S M S S S S S S S S S M U S M M Oxalic Acid U U M S S S U S S S S S U S U S S S S S S S S U M S S Perchloric Acid 10 U U S U U S M M M U M S M M M S...

Page 52: ...U S U S S U U Toluene S S U U S S M U S U U S U U U S U M U U U S U S U U M Trichloroacetic Acid U U U S S U M S U S U U S M M S S U U S U U U M U Trichloroethane S U M U U S U U U U U U U U U S U S S Trichloroethylene U U U U S U U U U U U U U U S U U S Trisodium Phosphate S M S S S S S S S Tris Buffer neutral pH U S S S S S S S S S S S S S S S S S S S S S S S S S Triton X 100 S S S S S S S S S S...

Page 53: ... under the stress of centrifugation when in doubt we recommend pretesting sample lots Polyethyleneterephthalate Key S Satisfactory M Moderate attack may be satisfactory for use in centrifuge depending on length of exposure speed involved etc suggest testing under actual conditions of use U Unsatisfactory not recommended Performance unknown suggest testing using sample to avoid loss of valuable mat...

Page 54: ... 9 THERMO 1 866 984 3766 Canada 1 866 984 3766 Austria 43 1 801 400 Belgium 32 2 482 30 30 Germany 08001 536 376 49 6184 90 6940 France 33 2 2803 2180 33 2 2803 2000 Italy 39 02 02 95059 341 Netherlands 31 76 571 4440 Nordic Baltic Countries 35 89 329 100 Russia 7 812 703 42 15 ...

Page 55: ...09 18 Portugal 34 932 23 09 18 Switzerland 41 44 454 12 12 UK Ireland 44 870 609 9203 China 86 21 6865 4588 86 10 8419 3588 India 91 22 6716 2200 Japan 81 45 453 9220 Other Asian Countries 852 2885 4613 Latin America 1 866 984 3766 Other Countries 49 6184 90 6940 33 2 2803 2180 ...

Page 56: ...ntervalls 6 2 CLICKSeal 5 3 Contact Information D 1 Cycle Counter 4 5 D Decontamination 6 4 Disinfection 6 3 F Fill Level 5 2 H Heraeus 1 2 I Improper Loading 4 3 M Maintenance 6 1 Maximum Loading 4 4 P Precautions iii Preface iii Proper Loading 4 2 R RCF Values A 1 Removing the Rotor 3 3 Rotor Installation 3 2 Rotor Loading 4 1 Rotor Specifications 1 1 S Scope of Supply iii Service 6 6 Service Li...

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