Thermo Scientific Savant Speedvac ISS110, Instruction Manual

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Concentrating large quantities of organic solvents, such as hexane, chloroform,
dichloromethane, or acetonitrile from extraction or elution systems are best suit-
ed to the AES1010 and AES2010 SpeedVac Systems.
When processing hazardous samples, install the SCT
120
Chemical Trap on the
exhaust port to capture evaporated solvent. This trap, installed in addition to the
integral refrigerated trap, provides more complete solvent trapping. The SCT120
accepts a variety of disposable cartridges to absorb volatile exhaust. Order the
DTK
120
R Chemical Trap Kit, which includes the trap, disposable cartridge, tubing,
and fittings. These activated-charcoal cartridges trap radioactivity. (The same
technology is commonly used in fume hood holders.)
To install a trap on the exhaust port, remove the muffler assembly from the
VAPOR EXHAUST PORT. Attach a piece of vacuum tubing to this fitting. Attach
the other end to the chemical trap. (Follow enclosed instructions supplied with
the DTK
120
R kit).
The chemical trap requires periodic checking to ensure its continued effective-
ness. Refer to the manual shipped with the trap.
When concentrating samples in ammonium hydroxide, install the ANT100
Ammonia Neutralizing Trap. The disposable ANS121 (Ammonia Neutralizing
Solution) bottles, which screw onto the ANT100, neutralize ammonia gas,
preventing unpleasant odors in the laboratory.
The CC
120
/DX Deluxe Convenience Cart is a useful accessory for the envi-
ronmental lab. The SpeedVac ® resides on the top shelf, while any additional trap-
ping apparatus are placed on the bottom shelf. Addition of the cart produces a
completely mobile concentration system.
The model DVG
50
Digital Vacuum Gauge can also be installed. Insertion of
the vacuum gauge tube in the vacuum tubing, on the right hand side leading to
the glass condensation trap, provides vapor pressure reading that can be gener-
ally correlated with the dryness level of the sample. Experience with your specific
test samples will determine the correlation between vacuum levels and sample
dryness.
Test runs are necessary to determine the correct time settings for a given
procedure. To obtain data that is descriptive for both concentration (reducing a
large volume to a small volume) and drying (removing all the solvent), conduct
manual test runs, using the containers, the solvent and the volumes that you will
use for actual samples. Interrupt the run every 15 minutes to measure remaining
sample volume and sample temperature. Continue this test until the test sam-
ples are completely dry. Conduct additional runs at different drying settings.
4.1 SOLVENT HANDLING
4.3 DEVISING PROTOCOLS
4
4.2 ACCESSORIES