Section 15- Troubleshooting
Detector saturation- nucleic acid
measurement
Detector saturation- Bradford
measurement
A spectrum that is very “un-smooth” or “ragged” can be caused by insufficient light intensity reaching the spectrometer. If you suspect
that this is occurring, refer to the “Technical Service” section for instructions on how to contact NanoDrop Technologies and what
information must be sent to allow for diagnosing the problem.
Technical Service
If after referring to the above troubleshooting tips you are unable to resolve your problem, please contact your local distributor or
NanoDrop Technologies at
for help. The following information will be very helpful:
JPG image of Utilities and Diagnostics module
To get this, open this module and select ‘OK’ to initialize the module. Select ‘Intensity Check’. Once the spectrum has been created,
choose File
Æ
Save Window as shown below. Save to your hard drive and email as an attachment to your distributor or to
.
Application Module Screen Captures
Screen captures of the actual spectrum as seen on your PC are of great use in diagnosing problems. Making a screen capture is quite
easy. When in an application module, press ‘Alt+Print Screen’. This copies the highlighted screen window to the PC’s clipboard. Next,
paste this screen capture into MS Word, MS Paint (this program usually comes standard with the PC and can usually be found in the
Start
Æ
Accessories menu), or other graphics programs. Save this as a .jpg or .doc file and send as email attachment to your
distributor or to
.
Data Archive Files
If you have questions about your data, please send the archive file containing the suspect data as an email attachment to your
distributor or to
. The archived file can be found at
C:\NanoDrop Data
Æ
User name
Æ
Application Module
(
BCA
Protein, Bradford, Cell Culture, Protein Lowry, Proteins and Labels, MicroArray, Nucleic Acid, Protein A-280, UV-Vis or UV-Vis HiAbs).
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