Thermo Scientific KingFisher Pure RNA Blood Kit
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Thermo Fisher Scientific
Protocols and
Pipetting Instructions
Before beginning the RNA purification protocol, carefully read through the
Thermo Scientific™ KingFisher™ Flex User Manual (Cat. No. N07669), the
Thermo Scientific™ KingFisher™ Duo User Manual (Cat. No. N12420) or
the
Thermo Scientific™ KingFisher™ mL User Manual (Cat. No. 1508260),
and the
Thermo Scientific™ BindIt™ Software for KingFisher Instruments
version 3.2 User Manual (Cat. No. N07974).
BindIt Software protocols for the KingFisher Pure RNA Blood Kit can be found
in BindIt Software 3.2 and at
www.thermoscientific.com/kingfisher
Handling of KingFisher Magnetic Beads
A homogeneous distribution of the KingFisher Magnetic Beads in the container
is essential before the beads are transferred to the wells or tubes in order
to ensure a high consistency between the wells or tubes. To gain complete
resuspension of the beads, shake the container vigorously or vortex briefly.
Avoiding Ribonuclease (RNase)
Contamination
RNA purity and integrity is essential for downstream applications. RNase is a
ubiquitously found enzyme, which degrades RNA. RNases are highly stable
contaminants found in any laboratory environment. Keep all kit components
tightly sealed when not in use.
Skin is a common source of RNases. Always wear gloves when handling
reagents and RNA samples. Use sterile, RNase-free pipette tips when working
with RNA. Remove RNase contamination from work surfaces and non-disposable
items (pipettes, centrifuges) with reagents designed to remove RNase.
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