Thermo Fisher Scientific
24
Thermo Scientific KingFisher Plant DNA Kit Instruction Manual
Protocols and Pipetting Instructions
Homogenization of sample material
4. Place a Thermo Scientific KingFisher Flex 96 tip comb
for deep well magnets on a
Tip Plate
(that is, an
empty KingFisher Flex 96 KF plate).
5. Start the KF_PlantDNA_Flex96 protocol with the
KingFisher Flex 96 and load the plates.
Switch on the KingFisher Flex and make sure that you
are using the KingFisher Flex 96 deep well head and
heating block. Connect the PC with BindIt Software 3.2
to the KingFisher Flex. Start the KF_PlantDNA_Flex96
protocol. Insert the Tip Plate and the filled plates into the
instrument as indicated on the KingFisher Flex display.
After all the plates have been loaded into the instrument,
the protocol will begin.
When the KingFisher Flex is to be run as a standalone
instrument, transfer the KF_PlantDNA_Flex96
protocol to the KingFisher Flex. The instructions for
transferring the protocol can be found in Chapter 4:
“
Using the software
” in the
BindIt Software for KingFisher
Instruments version 3.2 User Manual
.
6. After the run is completed, remove the plates and store
the purified DNA.
When the protocol is completed, remove the plates
according to the instructions on the KingFisher Flex
display and turn off the instrument. Store the purified
DNA accordingly. The purified DNA is ready for use in
downstream applications.
The final DNA concentration in the Elution Buffer may
increase if the purified DNA is eluted into a smaller
volume of the buffer than is recommended, but this can
slightly reduce the overall DNA yield.
