Thermo Fisher Scientific
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Thermo Scientific KingFisher Plant DNA Kit Instruction Manual
Chapter 2
Product Description
The KingFisher Plant DNA Kit is designed for rapid
automated purification of DNA from plant materials
using KingFisher instruments. The DNA purified with the
KingFisher Plant DNA Kit is of high quality and free of
proteins, nucleases, and other contaminants or inhibitors.
It is therefore suitable for direct use in many different
downstream applications, such as PCR (polymerase
chain reaction), restriction analysis and in several other
enzymatic reactions.
The KingFisher Plant DNA Kit is developed for
the purification of DNA from plant material using
paramagnetic particles. The purification process requires
no phenol/chloroform extraction or alcohol precipitation
and needs very little hands-on time. The reagents and
specific plastic consumables are designed to work with
the KingFisher Flex or KingFisher Duo magnetic particle
processors as part of an integrated system. The KingFisher
Plant DNA Kit is only intended for research use, not
for clinical or diagnostic use. The user is responsible for
validating the performance of the KingFisher instrument
and the KingFisher Plant DNA Kit for any particular use,
because the performance of the kits has not been validated
for any specific organism.
The KingFisher Plant DNA Kit uses magnetic-particle
technology for DNA purification. The Thermo Scientific
KingFisher technology combines the speed and efficiency
of DNA purification with easy handling of magnetic
particles. It is recommended that fresh, frozen, dried
Introduction
Intended use
Principle and
procedure
