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© 201

4

Thermo Fisher Scientific Inc. All rights reserved.

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Thermo Fisher Scientific Inc. provides this document to its customers with a product purchase to use in the
product operation. This document is copyright protected and any reproduction of the whole or any part of this
document is strictly prohibited, except with the written authorization of Thermo Fisher Scientific Inc.

The contents of this document are subject to change without notice. All technical information in this
document is for reference purposes only. System configurations and specifications in this document supersede
all previous information received by the purchaser.

Thermo Fisher Scientific Inc. makes no representations that this document is complete, accurate or error-
free and assumes no responsibility and will not be liable for any errors, omissions, damage or loss that
might result from any use of this document, even if the information in the document is followed
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Sale shall govern all conflicting information between the two documents.

Original instruction manual 20057760-

c

printed in

08 /

201

4

.

Instruction manual 20057761-

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is a translation of 20057760-

c

.

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Summary of Contents for Heraeus Multifuge 4KR

Page 1: ...20057761 c 08 2014 Thermo Scientific Heraeus Multifuge 4KR Instruction Manual Visit us online to register your warranty www thermoscientific com labwarranty ...

Page 2: ...of the whole or any part of this document is strictly prohibited except with the written authorization of Thermo Fisher Scientific Inc The contents of this document are subject to change without notice All technical information in this document is for reference purposes only System configurations and specifications in this document supersede all previous information received by the purchaser Therm...

Page 3: ...auch in Übereinstimmung mit allen einschlägigen Bestimmungen der Richtlinie 2004n 08 EG über elektromagnetische Vefträglichkeit The machinery is in accordance with all relevant terms of directives for electromagnetic compatibility 2004 108 EC Die Schutzziele der Niederspannungsrichtlinie 2006 9s EG werden eingehalten The protection goals of the directive for low voltage 2006 95 EC are met Angewand...

Page 4: ... agreements with recycling and disposal companies in all EU Member States for the recycling and disposal of this device For information on recycling and disposal companies in Germany and on the products of Thermo Fisher Scientific which fall under the RoHS Directive Restriction of the use of certain hazardous substances in electrical and electronic equipment please visit the website www thermo com...

Page 5: ...ng up 2 2 Location 2 2 Setting up 2 2 Transporting the Centrifuge 2 3 Mains Connection 2 4 Storage 2 4 Chapter 3 Control Panel 3 1 Control Panel 3 2 Keys 3 2 Chapter 4 Operation 4 1 Switch on Centrifuge 4 2 Centrifuge Door 4 2 Rotor Installation 4 3 Entering Parameters 4 4 Centrifugation 4 8 Temperature Adaptation during Standstill 4 9 Short term Centrifugation 4 9 Removing the Rotor 4 9 Aligning ...

Page 6: ...5 4 Service of Thermo Fisher Scientific 5 5 Shipping and deposing of accessories 5 6 Chapter 6 Troubleshooting 6 1 Mechanical Emergency Door Release 6 2 Troubleshooting by User 6 3 When to contact a Service Technician 6 5 Chemical Compatibility Chart A 1 Contact Information B 1 ...

Page 7: ... manual will result in the expiration of the sellers warranty Scope of Supply If any parts are missing please contact your nearest Thermo Fisher Scientific representative Article Number Quantity Check 75004461 Heraeus Multifuge 4KR Centrifuge 1 Power supply cable 1 20360073 Allen wrench 1 50127698 CD with manual 1 This symbol refers to general hazards CAUTION means that material damage could occur...

Page 8: ...article size suspended in a liquid Maximum sample density at maximum speed 1 2 Accident Prevention This symbol means that both castor breaks must be closed before the run g cm 3 Prerequisite for the safe operation of the Heraeus Multifuge 4KR is a work environment in compliance with standards directives and trade association safety regulations and proper instruction of the user The safety regulati...

Page 9: ...onents of the rotor and do not make any changes to the mechanical components Use only with rotors which have been properly installed Follow the instructions in section Rotor Installation on page 4 3 Use only with rotors which have been loaded properly Follow the instructions given in the rotor manual Never overload the rotor Follow the instructions given in the rotor manual Never open the lid unti...

Page 10: ...rifuge toxic or radioactive materials or any pathogenic micro organisms without suitable safety precautions When centrifuging microbiological samples from the Risk Group II according to the Bio safety Manual of the World Health Organization WHO aerosol tight biological seals have to be used For materials in a higher risk group extra safety measures have to be taken If toxins or pathogenic substanc...

Page 11: ...ion and Description Contents Characteristics of the Heraeus Multifuge 4KR on page 1 2 Technical Data on page 1 3 Directives Standards and Guidelines on page 1 3 Functions and Features on page 1 4 Mains Supply on page 1 5 Rotor Selection on page 1 5 ...

Page 12: ...ge for the separation of blood components A special wind shield rotor allows to process up to eight quintuple blood bag systems The Heraeus Multifuge 4KR is equipped with various safety features The housing and rotor chamber consist of steel plate the interior of armor steel while the front panel is made of high impact resistant plastic The door is equipped with a view port and a lock The door of ...

Page 13: ... Category II Pollution Degree 2 Heat Dissipation 9200 BTU h IP 20 Run Time Unlimited Max Speed nmax 10000 rpm Depending on the Rotor Min Speed nmin 300 rpm Maximum RCF Value at nmax 15317xg Maximum Kinetic Energy 63 4 kJ Noise Level at maximum Speed 61 dB A temperature Setting Range 9 C to 40 C Dimensions Height Width Depth 700 mm 680 mm 720 mm Weight without Rotor 236 kg Table 1 2 Directives Stan...

Page 14: ...ion and 9 Braking Curves Rotor Recognition Automatic Imbalance Detection SMARTspin Electronic contingent on Rotor and Speed Door Lock Motor assisted Door Looking Function Feature Program Display 1 9 Free Program Selection Operating Memory Memory Capacity for Set Values PULSE Mode Acceleration Braking Profiles 1 slow 9 fast Acceleration Deceleration Curve Speed Selection Adjustable from 300 rpm to1...

Page 15: ... Speed of Rotor upon Key Release Centrifuge stops with maximum Deceleration Power Diagnostic Messages Alternating Display rotor Maximum Speed or RCF Speed selected exceeds max Speed of the rotor Door has not been lifted off the Lock during opening Display Lift Lid Manual Lifting of Door required General Centrifuge Malfunction Error Messages with ERROR Codes see Troubleshooting by User on page 6 3 ...

Page 16: ...ientific Multifuge 4KR 2 1 2 Before Use Contents Before Setting up on page 2 2 Location on page 2 2 Setting up on page 2 2 Transporting the Centrifuge on page 2 3 Mains Connection on page 2 4 Storage on page 2 4 ...

Page 17: ...ifuging The supporting structure must be stable and free of resonance The supporting structure must be suitable for horizontal setup of the centrifuge The centrifuge should not be exposed to heat and strong sunlight The set up location must be well ventilated at all times Setting up 1 Place the pallet with the centrifuge so that you have at leased 2 m room in front of the pallet 2 Release the rail...

Page 18: ...to lift the centrifuge The centrifuge can be damaged by impacts Transport the centrifuge upright and if at all possible in packaging Rails Screws WARNING Due to the centrifuges weight it must be controlled by several people when rolling of the pallet Do not stand in front of the centrifuge when rolling down for the centrifuge might run you over Note The packaging is an one way packaging Contact a ...

Page 19: ... complies with the safety standards of your country 4 Make sure that the voltage and frequency correspond to the figures on the rating plate 5 Establish the connection to the power supply with the connecting cable Storage Before storing the centrifuge and the accessories it must be cleaned and if necessary disinfected and decontaminated Store the centrifuge in a clean dust free location Be sure to...

Page 20: ...Thermo Scientific Multifuge 4KR 3 1 3 Control Panel Contents Control Panel on page 3 2 Keys on page 3 2 ...

Page 21: ...d on Emergency Release see Mechanical Emergency Door Release on page 6 2 Pulse By pressing the Key the Centrifuge starts immediately and accelerates up to the End Speed Releasing the Key initiates a Stopping Process at the highest Braking Curves UP By pressing the Key you change the Value in the Display Bucket Use the Key in Order to have all available Bucket Types displayed in Succession Pre Temp...

Page 22: ...age 4 2 Centrifuge Door on page 4 2 Rotor Installation on page 4 3 Entering Parameters on page 4 4 Centrifugation on page 4 8 Temperature Adaptation during Standstill on page 4 9 Short term Centrifugation on page 4 9 Removing the Rotor on page 4 9 Aligning the Centrifuge on page 4 10 ...

Page 23: ...ess the key Close Door 2 Close the door by pressing down on it lightly in the middle or on both sides of it Two locks close the door completely WARNING The performance of the gas springs might diminish after some time Do not place you hand in the door opening when the door is not totally open WARNING Do not reach into the crack between the door and the housing The door is drawn shut automatically ...

Page 24: ...fuge spindle clockwise Hold the rotor with the other hand into position 5 Check if the rotor is properly installed by lifting it slightly on the handle 6 If available close the rotor with the rotor lid 7 Close the centrifuge door CAUTION Unapproved or incorrectly combined accessories can cause serious damage to the centrifuge CAUTION Do not force the rotor onto the centrifuge spindle WARNING Check...

Page 25: ...eaking curve to change the breaking curve Curve number 1 is the slowest and curve number 9 the fastest 2 The value is stored when the value stops flashing Pre selecting Speed RCF 1 Press the key below the field speed RCF value Next to the display a light shows the actual unit 2 The display shows the speed or the RCF value depending on the display setting Press the key to toggle between the two mod...

Page 26: ...um RCF value is related to the maximum radius of the tube opening Remember that this value is reduced depending on the tubes and adapters used This can be accounted for in the calculation above if required K Factor The k factor is an information on the sedimentation capacity of a rotor You can calculate the required sedimentation time of each rotor with this formula t sedimentation time in hours K...

Page 27: ...can limit the run time to 9 59 1 Press the key for 1 second The display shows 2 Press the key below the field speed upwards The display shows 3 Press the key below the field run time upwards You can either choose the limited time mode 0 00 or the standard mode 00 0 Pre selecting the Temperature You can pre select temperatures between 9 C and 40 C 1 Press the key below the field temperature 2 Enter...

Page 28: ...ed to calculated the RCF value and control the temperature 1 Press the key below the field speed RCF value until the correct bucket is shown 2 The value is stored and the display shows the speed or RCF value Setting up a Program 1 Press the key below the field program until the correct number in the program memory is shown The display flashes 2 Press the key All displays flash 1 Enter the program ...

Page 29: ...m speed or RCF value the display will flash for 15 seconds between rotor and the maximum value for the installed rotor a Please enter a new value Within this period it is possible to accept this value by again pressing the key The centrifugation is then continued b After 15 sec the rotor will be decelerated Always wait until the rotor has come to a stop without braking By opening and closing the d...

Page 30: ...oor and remove the sample Temperature Adaptation during Standstill The temperature cannot be adapted until the rotor has been positively identified For this the centrifuge must have accelerated to over 300 rpm The speed display will then show End When the rotor is not recognized door closed and key not yet pressed speed display 0 the centrifuge responds by ensuring that the sample cannot freeze re...

Page 31: ...ff the centrifuge put the mains switch to 0 Audible Alarm Error messages are accompanied by an audible alarm Press any key to silence the audible alarm The end of run can also be accompanied by an audible alarm To switch off this signal proceed as follows 1 Press the key for 1 second The display shows the current setting 2 Use the key below the field run time until it says ON in the display if the...

Page 32: ... function can be used B The program memory is protected against changes The user can use all programs but cannot change them PULSE and the pre temp function can be used C Program memory and RAM are protected against changes All set parameters are blocked but the actual program can be operated as often as needed with the control keys All set values can be seen with the arrow keys The selected progr...

Page 33: ... 1 5 Maintenance and Care Contents Cleaning Intervals on page 5 2 Cleaning on page 5 2 Disinfection on page 5 4 Decontamination on page 5 4 Service of Thermo Fisher Scientific on page 5 5 Service of Thermo Fisher Scientific on page 5 5 ...

Page 34: ...the centrifuge 2 Turn off the centrifuge 3 Pull out the power supply plug 4 Release the rotor 5 Grasp the rotor with both hands and lift it vertically off the centrifuge spindle Maintenance Recommended Interval Clean Rotor Chamber Daily or when polluted Clean Rotor Daily or when polluted Accessories Daily or when polluted Cabinet Once per month Ventilation Holes Every six months CAUTION Refrain fr...

Page 35: ...proceed as follows 1 Turn off the centrifuge 2 Pull out the power supply plug 3 Remove the lateral fastening screws and demount the venting grid Abbildung 5 1 Open centrifuge 4 Clean the cooling fins carefully with a brush Make sure you do not deform the cooling fins 5 Place the venting grid again and fastened the screws thoroughly CAUTION When cleaning do not allow liquids especially organic solv...

Page 36: ...econtaminate the centrifuge immediately whenever contaminating material has spilled during centrifugation WARNING Infectious material can get into the centrifuge when a tube breaks or as a result of spills Keep in mind the risk of infection when touching the rotor and take all necessary precautions In case of contamination make sure that others are not put at risk Decontaminate the affected parts ...

Page 37: ...rding to the applicable guidelines Service of Thermo Fisher Scientific Thermo Fisher Scientific recommends having the centrifuge and accessories serviced once a year by an authorized service technician The service technician checks the following the electrical equipment the suitability of the set up site the lid lock and the safety system the rotor the fixation of the rotor and the drive shaft The...

Page 38: ...fic Shipping and deposing of accessories WARNING Before shipping or deposing centrifuges and accessories you have ton cleanand if necessary disinfect or decontaminate everything Before storing the centrifuge and the accessories it must be cleaned and if necessary disinfected and decontaminated ...

Page 39: ...Thermo Scientific Multifuge 4KR 6 1 6 Troubleshooting Contents Mechanical Emergency Door Release on page 6 2 Troubleshooting by User on page 6 3 When to contact a Service Technician on page 6 5 ...

Page 40: ...ss lasts much longer than usual Proceed as follows 1 Make sure the rotor has stopped view port in the door 2 Pull out the power supply plug 3 On both sides of the housing is a white plastic plug which you can pry out of the back plate with a screwdriver or a knife Pull the release cords attached to it at the same time to trigger the mechanical door release The door will open and the samples can be...

Page 41: ...entrifuge does not open Restart the centrifuge The emergency door release enables you to retrieve your samples If the message appears again inform a service technician Exceptionally running noise Check the load placed in the rotor Check that the rotor cross bolts are well greased Restart the centrifuge If the message appears again inform a service technician Message bAl appears in display Rotor st...

Page 42: ...ogram error in the memory Restart the centrifuge If the message appears again inform a service technician E 03 The run does not start or the centrifuge runs down without being braked Error in speed entry Restart the centrifuge If the message appears again inform a service technician E 04 The run does not start or the centrifuge runs down without being braked Temperature measurement malfunction sen...

Page 43: ...e identified Restart the centrifuge If the message appears again inform a service technician E 15 The run does not start or the centrifuge runs down without being braked The check sum in the NV RAM is incorrect E 17 Door does not open Door is blocked or jammed Press the door down once at the front in the center and then actuate the key again Otherwise see Mechanical Emergency Door Release on page ...

Page 44: ...tact a Service Technician 6 6 Multifuge 4KR Thermo Scientific 2 Afterwards the different programs are tested as well 3 Communicate the software version to the service technician Note The values given are only examples Program test ...

Page 45: ... U S M U U S S U S U S U Ammonium Acetate S S U S S S S S S S S S S U S S S S S S S S S S Ammonium Carbonate M S U S S S S S S S S S S S U U S S S S S S M S S S Ammonium Hydroxide 10 U U S U S S M S S S S S S U M S S S S S S S S S M S Ammonium Hydroxide 28 U U S U S U M S S S S S U S U M S S S S S S S S S M S Ammonium Hydroxide conc U U U U S U M S S S U S U U S S S M S S S S U Ammonium Phosphate ...

Page 46: ... S S S S M U S S Acetic Acid Glacial S S U U S S U M S U S U U U U U M S U M U S U U S U Acetic Acid 5 S S M S S S M S S S S S M S S S S S S S M S S M S S M Acetic Acid 60 S S U U S S U S M S U U M U S M S M S M S M U S M U Ethyl Acetate M M U U S S M M S S U S U M U U S S U U S M M S U U Ethyl Alcohol 50 S S S S S S M S S S S S U S U S S S S S S S S M S M U Ethyl Alcohol 95 S S S U S S M S S S S ...

Page 47: ... U S S U S Sodium Chloride 10 S S S S S S S S S S S S S S S S S S M S Sodium Chloride sat d U S U S S S S S S S S S S S S S M S Carbon Tetrachloride U U M S S U M U S U U S U M U S S M M S M M M M U S S Aqua Regia U U U U U U U U U U U S M Solution 555 20 S S S S S S S S S S S S S S S S S S S S Magnesium Chloride M S S S S S S S S S S S S S S S S S S S S S M S S S Mercaptoacetic Acid U S U S M S S...

Page 48: ...U U U U S U M U U M U U U M M U M S U U S U S Phenol 5 U S U S M M S U M U U S U M S M S U U S U M M M S Phenol 50 U S U S U M S U M U U U U U S U M U U S U U U M S Phosphoric Acid 10 U U M S S S U S S S S U S S S S S S S S S U M U S S Phosphoric Acid conc U U M M U S M S U U M M S S S M S M S U M U S Physiologic Media Serum Urine M S S S S S S S S S S S S S S S S S S S S S S S Picric Acid S S U S...

Page 49: ...tral pH U S S S S S S S S S S S S S S S S S S S S S S S S S Triton X 100 S S S S S S S S S S S S S S S S S S S S S S S S S Urea S U S S S S S S S M S S S S S S S M S S Hydrogen Peroxide 10 U U M S S U U S S S U S S S M U S S S S S S M S U S Hydrogen Peroxide 3 S M S S S S S S S S S S S S M S S S S S S S S S S Xylene S S U S S S M U S U U U U U U M U M U U U S U M S U S Zinc Chloride U U S S S S U ...

Page 50: ...A Chemical Compatibility Chart A 6 Multifuge 4KR Thermo Scientific ...

Page 51: ...803 2180 Italy 39 02 95059 552 Netherlands 31 76 579 55 55 Nordic Baltic Countries CIS 358 9 329 10200 Russia 7 812 703 42 15 Spain Portugal 34 932 23 09 18 Switzerland 41 44 454 12 22 UK Ireland 44 870 609 9203 China 800 810 5118 400 650 5118 India 91 22 6716 2200 Japan 81 3 5826 1616 Other Asian Countries 852 2885 4613 Australia 61 39757 4300 New Zealand 64 9 980 6700 Other Countries 49 6184 90 ...

Page 52: ...actor 4 5 Explanation of RCF Value 4 5 F Features 1 4 I Imbalance Indicator 4 8 K Keys 3 2 L Location 2 3 M Mains Connection 2 4 Mains Supply 1 5 2 4 Maintenance 5 1 O Operation 4 1 P Precautions v Preface iii Preselecting RCF Value 4 4 Preselecting Speed 4 4 Preselecting the Temperature 4 6 Preset Run Time 4 9 Pre warming or Pre cooling 4 7 Pre warming or Pre cooling the Centrifuge 4 7 R Removing...

Page 53: ... Multifuge 4KR Thermo Scientific Stopping the Centrifugation Program 4 9 Storage 2 4 Switch on Centrifuge 4 2 T Technical Data 1 3 Temperature Adaptation 4 9 Transporting the Centrifuge 2 3 Trouble Shooting 6 1 ...

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