Thermo Scientific Heraeus Cryofuge 5500i Instruction Manual Download Page 14

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Cryofuge 5500i

Thermo Scientific

Introduction and Description

Refrigerants

Refrigerants

The Cryofuge 5500i is supplied without a rotor.

Various Thermo Scientific rotors are available to choose from.

The technical data of the rotors and the corresponding adapters and reduction sleeves for various
commercially available containers can be found in the corresponding rotor operating manuals.

For more information visit our website at: http://www.thermo

fisher

.com

LH-4000

This rotor is not available for the 120 V centrifuge.

75006475

with round Buckets 1000

75006477

Double rectangular Bucket DoubleSpin

2 x 250

75006478

LH-4000W

75006476

with round Buckets 1000

75006477

Double rectangular Bucket DoubleSpin

2 x 250

75006478

with double Blood Bag Bucket

This rotor is not available for the 120 V centrifuge.

75006436

BIOshield

4 x 250

75006435

HIGHPlate

5 x 5 Platten

75006444

Diagnostik

75006480

HIGHConic

6 x 50

75003057

LAC-250 6 x 250

75006483

Rotor Selection

Table 1-

Refrigerants

Article No.

7500

4471

Thermo Scientific Cryofuge 5500i

7500

4475

7500

4479

Centrifuge

Refrigerant

Quantity

Pressure

GWP

Thermo Scientific Cryofuge 5500i

R-134A

1 kg

21 bar

1430

1.43 t

1 kg

21 bar

1430

1.43 t

1 kg

21 bar

1430

1.43 t

Contains fluorinated greenhouse gases in a hermetically sealed system.

Summary of Contents for Heraeus Cryofuge 5500i

Page 1: ...Thermo Scientific Heraeus Cryofuge 5500i Instruction Manual 20057781 e 07 2020 Visit us online to register your warranty thermofisher com labwarranty ...

Page 2: ...WEEE Conformity This product is subject to the regulations of the EU Waste Electrical Electronic Equipment WEEE Directive 2012 19 EU It is marked by the following symbol ...

Page 3: ...e Setting up 2 2 Setting up 2 2 Aligning the Centrifuge 2 4 Transporting the Centrifuge 2 4 Location 2 4 Mains Connection 2 5 Adjust Voltage and Frequency 2 6 Storage 2 6 Chapter 3 Control Panel 3 1 Control Panel 3 2 Keys 3 2 Chapter 4 Operation 4 1 Switch on Centrifuge 4 2 Centrifuge Door 4 2 Rotor Installation 4 3 Entering Parameters 4 4 Centrifugation 4 8 Temperature Adaptation during Standstil...

Page 4: ... Cleaning Intervals 5 2 Cleaning 5 2 Cleaning the Venting Slots 5 3 Disinfection 5 3 Decontamination 5 4 Service of Thermo Fisher Scientific 5 5 Chapter 6 Troubleshooting 6 1 Mechanical Emergency Door Release 6 2 Troubleshooting by User 6 3 When to contact a Service Technician 6 5 ...

Page 5: ...this instruction manual will result in the expiration of the seller s warranty Scope of Supply If any parts are missing please contact your nearest Thermo Fisher Scientific representative Article Number Quantity Check Centrifuge Cryofuge 5500i 1 Power supply cable 1 50127698 CD with manual 1 This symbol refers to general hazards CAUTION means that material damage could occur WARNING means that inj...

Page 6: ...revention Precautions g cm 3 Prerequisite for the safe operation of the Heraeus Cryofuge 5500i is a work environment in compliance with standards directives and trade association safety regulations and proper instruction of the user The safety regulations contain the following basic recommendations Maintain a radius of at least 30 cm around the centrifuge Implementation of special measures which e...

Page 7: ...uctions given in the rotor manual Never open the lid until the rotor has come to a complete stop and this has been confirmed in the display The lid emergency release may be used in emergencies only to recover the samples from the centrifuge e g during a power failure see section Mechanical Emergency Door Release on page 6 2 The performance of the gas springs might diminish after some time Check th...

Page 8: ... toxic or radioactive materials or any pathogenic micro organisms without suitable safety precautions When centrifuging microbiological samples from the Risk Group II according to the Bio safety Manual of the World Health Organization WHO aerosol tight biological seals have to be used For materials in a higher risk group extra safety measures have to be taken If toxins or pathogenic substances hav...

Page 9: ...Description Contents Characteristics of the Cryofuge 5500i on page 1 2 Technical Data on page 1 3 Directives Standards and Guidelines on page 1 4 Functions and Features on page 1 4 Mains Supply on page 1 5 Refrigerants on page 1 6 Rotor Selection on page 1 6 ...

Page 10: ...ation of blood components A special wind shield rotor allows to process up to eight quintuple blood bag systems The Cryofuge 5500i is equipped with various safety features The housing and rotor chamber consist of steel plate the interior of armor steel while the front panel is made of high impact resistant plastic The door is equipped with a view port and a lock The door of the centrifuge can only...

Page 11: ...200 BTU h 6300 BTU h IP 20 20 20 Running Time unlimited unlimited unlimited Max Speed nmax 10000 rpm depending on the rotor 10000 rpm depending on the rotor 10000 rpm depending on the rotor Min Speed nmin 300 rpm 300 rpm 300 rpm Maximum RCF Value at nmax 15317 x g 15317 x g 15317 x g Maximum Kinetic Energy 63 4 kJ 63 4 kJ 63 4 kJ Noise Level at maximum Speed 61 dB A 61 dB A 61 dB A Temperature Set...

Page 12: ... Memory The most recent Data is saved Functions RCF Selection Temperature Regulation during Standstill Pre Temp and PULSE Acceleration Braking Profiles 9 Acceleration and 9 Braking Curves Rotor Recognition Automatic Imbalance Detection SMARTspin Electronic contingent on Rotor and Speed Door Lock Motor assisted Door Looking Region Standards and Guidelines Europe 2006 42 EC Machinery Protective goal...

Page 13: ...art Key Stop Key PULSE Mode Pressing the Key activates maximum Acceleration up to the maximum permissible Speed of Rotor upon Key release Centrifuge stops with maximum Deceleration Power Diagnostic Messages Alternating Display rotor maximum Speed or RCF Speed selected exceeds max Speed of the Rotor Door has not been lifted off the Lock during Opening Display Lift Lid manual Lifting of door require...

Page 14: ...e rectangular Bucket DoubleSpinTM 2 x 250 75006478 LH 4000W 75006476 with round Buckets 1000 75006477 Double rectangular Bucket DoubleSpinTM 2 x 250 75006478 with double Blood Bag Bucket2 2 This rotor is not available for the 120 V centrifuge 75006436 BIOshield 4 x 250 75006435 HIGHPlate 5 x 5 Platten 75006444 DiagnostikTM 75006480 HIGHConic 6 x 50 75003057 LAC 250 6 x 250 75006483 Rotor Selection...

Page 15: ...e Contents Before Setting up on page 2 2 Setting up on page 2 2 Aligning the Centrifuge on page 2 4 Transporting the Centrifuge on page 2 4 Location on page 2 4 Mains Connection on page 2 5 Adjust Voltage and Frequency on page 2 6 Storage on page 2 6 ...

Page 16: ...If the order is incomplete please contact Thermo Fisher Scientific Setting up 1 Place the pallet with the centrifuge so that you have atleast 2 m room in front of the pallet 2 Release the rails from the pallet 3 Screw the rails onto the pallet in front of the centrifuge WARNING The centrifuge operated with a high kinetic energy Do not operate the centrifuge without a 30 cm 12 inch safety zone arou...

Page 17: ...rifuge is in its final position mount the enclosed locking stabilizers 9 Screw the threaded nuts down 10 Place the locking stabilizers centrally under the threaded rods and align the centrifuge All the locking stabilizers should carry uniformly check by a tilt test The casters are some 5 15 mm off the ground Threaded rod Lock nut Safety bracket Locking stabilizer Threaded nut Threaded nut WARNING ...

Page 18: ...possible in packaging Location The centrifuge should only be operated indoors The set up location must fulfill the following requirements A safety zone of at least 30 cm must be maintained around the centrifuge People and hazardous substances must be kept out of the safety zone while centrifuging The supporting structure must be stable and free of resonance The supporting structure must be suitabl...

Page 19: ... located on right side press 0 2 Plug the centrifuge into grounded electrical sockets only 3 Check whether the cable complies with the safety standards of your country 4 Make sure that the voltage and frequency correspond to the figures on the rating plate 5 Establish the connection to the power supply with the connecting cable WARNING UV rays reduce the stability of plastics Do not subject the ce...

Page 20: ...e switches on the back as shown in the table 3 Connect the centrifuge to the power supply again Storage Before storing the centrifuge and the accessories it must be cleaned and if necessary disinfected and decontaminated Store the centrifuge in a clean dust free location Be sure to place the centrifuge on its feet Avoid direct sunlight CAUTION Remove the power supply plug in order to prevent damag...

Page 21: ...Thermo Scientific Cryofuge 5500i 3 1 3 Control Panel Contents Control Panel on page 3 2 Keys on page 3 2 ...

Page 22: ...d on Emergency release see Mechanical Emergency Door Release on page 6 2 Pulse By pressing the key the centrifuge starts immediately and accelerates up to the end speed Releasing the key initiates a stopping process at the highest braking curves UP By pressing the key you change the value in the display Bucket Use the key in order to have all available bucket types displayed in succession Pre Temp...

Page 23: ...age 4 2 Centrifuge Door on page 4 2 Rotor Installation on page 4 3 Entering Parameters on page 4 4 Centrifugation on page 4 8 Temperature Adaptation during Standstill on page 4 9 Short term Centrifugation on page 4 9 Removing the Rotor on page 4 9 Aligning the Centrifuge on page 4 10 ...

Page 24: ...ess the key Close Door 2 Close the door by pressing down on it lightly in the middle or on both sides of it Two locks close the door completely WARNING The performance of the gas springs might diminish after some time Do not place you hand in the door opening when the door is not totally open WARNING Do not reach into the crack between the door and the housing The door is drawn shut automatically ...

Page 25: ...ifuge spindle clockwise Hold the rotor with the other hand into position 5 Check if the rotor is properly installed by lifting it slightly on the handle 6 If available close the rotor with the rotor lid 7 Close the centrifuge door CAUTION Unapproved or incorrectly combined accessories can cause serious damage to the centrifuge CAUTION Do not force the rotor onto the centrifuge spindle WARNING Chec...

Page 26: ...breaking curve to change the breaking curve Curve number 1 is the slowest and curve number 9 the fastest 2 The value is stored when the value stops flashing Preselecting Speed RCF 1 Press the key below the field speed RCF value Next to the display a light shows the actual unit key 2 The display shows the speed or the RCF value depending on the display setting Press the to toggle between the two mo...

Page 27: ...alue is related to the maximum radius of the tube opening Remember that this value is reduced depending on the tubes and adapters used This can be accounted for in the calculation above if required K Factor The k factor is an information on the sedimentation capacity of a rotor You can calculate the required sedimentation time of each rotor with this formula t sedimentation time in hours K clearin...

Page 28: ...Time Mode You can limit the run time to 9 59 1 Press the key for 1 second The display shows 2 Press the key below the field speed upwards The display shows 3 Press the key below the field run time upwards You can either choose the limited time mode 0 00 or the standard mode 00 0 Preselecting the Temperature You can preselect temperatures between 9 C and 40 C 1 Press the key below the field tempera...

Page 29: ...d to calculate the RCF value and control the temperature 1 Press the key below the field speed RCF value until the correct bucket is shown 2 The RCF value is stored and the display shows the speed or RCF value Setting up a Program 1 Press the key below the field program until the correct number in the program memory is shown The display flashes 2 Press the key All displays flash 1 Enter the progra...

Page 30: ...speed or RCF value the display will flash for 15 seconds between rotor and the maximum value for the installed rotor a Please enter a new value Within this period it is possible to accept this value by again pressing the key The centrifugation is then continued b After 15 seconds the rotor will be decelerated Always wait until the rotor has come to a stop without braking By opening and closing the...

Page 31: ...sing the key you can open the door and remove the sample Temperature Adaptation during Standstill The temperature cannot be adapted until the rotor has been positively identified For this the centrifuge must have accelerated to over 300 rpm The speed display will then show End When the rotor is not recognized door closed and key not yet pressed speed display 0 the centrifuge responds by ensuring t...

Page 32: ...m Error messages are accompanied by an audible alarm Press any key to silence the audible alarm The end of run can also be accompanied by an audible alarm To switch off this signal proceed as follows 1 Press the key for 1 second The display shows the current setting 2 Use the key below the field run time until it says ON in the display if the centrifuge should make an audible alarm after the run U...

Page 33: ... function can be used B The program memory is protected against changes The user can use all programs but cannot change them PULSE and the pre temp function can be used C Program memory and RAM are protected against changes All set parameters are blocked but the actual program can be operated as often as needed with the control keys All set values can be seen with the arrow keys The selected progr...

Page 34: ......

Page 35: ...00i 5 1 5 Maintenance and Care Contents Cleaning Intervals on page 5 2 Cleaning on page 5 2 Cleaning the Venting Slots on page 5 3 Disinfection on page 5 3 Decontamination on page 5 4 Service of Thermo Fisher Scientific on page 5 5 ...

Page 36: ...the centrifuge 2 Turn off the centrifuge 3 Pull out the power supply plug 4 Release the rotor 5 Grasp the rotor with both hands and lift it vertically off the centrifuge spindle Maintenance Recommended Interval Clean Rotor Chamber Daily or when polluted Clean Rotor Daily or when polluted Accessories Daily or when polluted Cabinet Once per month Ventilation Holes Every six months CAUTION Refrain fr...

Page 37: ...sure you do not deform the cooling fins 5 Place the venting grid again and fastened the screws thoroughly Disinfection Disinfect the centrifuge immediately whenever infectious material has spilled during centrifugation CAUTION When cleaning do not allow liquids especially organic solvents to get on the drive shaft or the bearings of the centrifuge Organic solvents break down the grease in the moto...

Page 38: ...pilled during centrifugation For general radioactive decontamination use a solution of equal parts of 70 ethanol 10 SDS and water 1 Open the centrifuge 2 Turn off the centrifuge 3 Pull out the power supply plug CAUTION Before using any cleaning or decontamination methods except those recommended by the manufacturer users should check with the manufacturer that the proposed method will not damage t...

Page 39: ...ution according to the applicable guidelines Service of Thermo Fisher Scientific Thermo Fisher Scientific recommends having the centrifuge and accessories serviced once a year by an authorized service technician The service technicians check the following the electrical equipment the suitability of the set up site the door lock and the safety system the rotor the fixation of the rotor and the driv...

Page 40: ......

Page 41: ...Thermo Scientific Cryofuge 5500i 6 1 6 Troubleshooting Contents Mechanical Emergency Door Release on page 6 2 Troubleshooting by User on page 6 3 When to contact a Service Technician on page 6 5 ...

Page 42: ...r has stopped view port in the door 2 Pull out the power supply plug 3 On both sides of the housing are two white plastic plugs which you can pry out of the back plate with a screwdriver or a knife Pull the release cords attached to it at the same time to trigger the mechanical door release The door will open and the samples can be removed Figure 6 1 Emergency Door Release 4 Push the release cords...

Page 43: ...ncy door release enables you to retrieve your samples If an error message appears again inform a service technician Exceptionally running noise Check the load placed in the rotor Check that the rotor cross bolts are well greased Restart the centrifuge If an error message appears again inform a service technician Message bAl appears in display Rotor stops with deceleration to standstill Imbalance d...

Page 44: ...r in the memory Restart the centrifuge If an error message appears again inform a service technician E 03 The run does not start or the centrifuge runs down without being braked Error in speed entry Restart the centrifuge If an error message appears again inform a service technician E 04 The run does not start or the centrifuge runs down without being braked Temperature measurement malfunction sen...

Page 45: ...r could not be identified Restart the centrifuge If an error message appears again inform a service technician E 15 The run does not start or the centrifuge runs down without being braked The check sum in the NV RAM is incorrect E 17 Door does not open Door is blocked or jammed Press the door down once at the front in the center and then actuate the key again Otherwise see Mechanical Emergency Doo...

Page 46: ...echnician 6 6 Cryofuge 5500i Thermo Scientific 2 Afterwards the different programs are tested as well 3 Communicate the software version to the service technician NV RAM Version 2 Cycle counter Note The values given are only examples Program test ...

Page 47: ...U U S S Formic Acid 100 S M U U U S M U U S S U S U S U Ammonium Acetate S S U S S S S S S S S S S U S S S S S S S S S S Ammonium Carbonate M S U S S S S S S S S S S S U U S S S S S S M S S S Ammonium Hydroxide 10 U U S U S S M S S S S S S U M S S S S S S S S S M S Ammonium Hydroxide 28 U U S U S U M S S S S S U S U M S S S S S S S S S M S Ammonium Hydroxide conc U U U U S U M S S S U S U U S S S ...

Page 48: ...S S S S U U Dioxane M S U U S S M M S U U S U M U U M M M U S S S S U U Ferric Chloride U U S M S M S S S S M U S S Acetic Acid Glacial S S U U S S U M S U S U U U U U M S U M U S U U S U Acetic Acid 5 S S M S S S M S S S S S M S S S S S S S M S S M S S M Acetic Acid 60 S S U U S S U S M S U U M U S M S M S M S M U S M U Ethyl Acetate M M U U S S M M S S U S U M U U S S U U S M M S U U Ethyl Alcoh...

Page 49: ... S S S S S S S S S S M S S S S S S S M S S S Calcium Hypochlorite M U S M M S M S S M S S S S M S M U S S Kerosene S S S S S S U S M U S U M M S M M M S S U S S U S Sodium Chloride 10 S S S S S S S S S S S S S S S S S S M S Sodium Chloride sat d U S U S S S S S S S S S S S S S M S Carbon Tetrachloride U U M S S U M U S U U S U M U S S M M S M M M M U S S Aqua Regia U U U U U U U U U U U S M Soluti...

Page 50: ...S S S S M S Oleic Acid S U S S S U U S U S S M S S S S S S S S S M U S M M Oxalic Acid U U M S S S U S S S S S U S U S S S S S S S S U M S S Perchloric Acid 10 U U S U U S M M M U M S M M M S U S S Perchloric Acid 70 U U U U U S U M U U M U U U M M U M S U U S U S Phenol 5 U S U S M M S U M U U S U M S M S U U S U M M M S Phenol 50 U S U S U M S U M U U U U U S U M U U S U U U M S Phosphoric Acid ...

Page 51: ... S U U U M U Trichloroethane S U M U U S U U U U U U U U U S U S S Trichloroethylene U U U U S U U U U U U U U U S U U S Trisodium Phosphate S M S S S S S S S Tris Buffer neutral pH U S S S S S S S S S S S S S S S S S S S S S S S S S Triton X 100 S S S S S S S S S S S S S S S S S S S S S S S S S Urea S U S S S S S S S M S S S S S S S M S S Hydrogen Peroxide 10 U U M S S U U S S S U S S S M U S S S...

Page 52: ...aterials under the stress of centrifugation When in doubt we recommend pretesting sample lots Key S Satisfactory M Moderate attack may be satisfactory for use in centrifuge depending on length of exposure speed involved etc Suggest testing under actual conditions of use U Unsatisfactory not recommended Performance unknown suggest testing using sample to avoid loss of valuable material ...

Page 53: ...tion of RCF Value 4 5 F Features 1 4 I Imbalance Indicator 4 8 K Keys 3 2 L Location 2 4 M Mains Connection 2 5 Mains Supply 1 5 2 6 Maintenance 5 1 O Operation 4 1 P Precautions P iv Preface P iii Preselecting RCF Value 4 4 Preselecting Speed 4 4 Preselecting the Temperature 4 6 Preset Running Time 4 9 Pre warming or Pre cooling 4 7 Pre warming or Pre cooling the Centrifuge 4 7 R Removing the Rot...

Page 54: ... Cryofuge 5500i Thermo Scientific Stopping the Centrifugation Program 4 9 Storage 2 6 Switch on Centrifuge 4 2 T Technical Data 1 3 Temperature Adaptation 4 9 Transporting the Centrifuge 2 4 Trouble Shooting 6 1 ...

Page 55: ...red trademark of General Electric Co Specifications terms and pricing are subject to change Not all products are available in all countries Please consult your local sales representative for details Shown pictures within the manual are examples and may differ considering the set parameters and language Pictures of the user interface within the manual are showing the English version as example Aust...

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