Thermo Scientific FOCUS GC, Instruction Manual

Page: 245 / 356

Chapter 14
SOP number: P0474/05/E - 02 May 2007
Instruction Manual 245
4. Column Installation
Install the test column as described in the How to Connect a Capillary
Column operating sequence following the instructions reported in:
• Preparing a Capillary Column
• Connecting to an PPKD Injector
5. Column Leak Check
Perform the column leak check following the he instructions reported in the
Manual Checking for Leaks operating sequence.
6. Column Evaluation
For pressure controlled injector (such as Split/Splitless Injector), the indirect
control of the column flow by automatic pressure programming relies on the
calculation and pre-determination of a column constant. The column
evaluation is an automatic measurement of the column resistance that
determines the column constant.
NOTE Column evaluation assumes there are no leaks in the column connection or gas plumbing lines.
Perform column evaluation following the instruction reported in the Performing a
Column Evaluation operating sequence.
7. Column Conditioning
CAUTION When performing column conditioning, the column should be connected only to the
injector leaving the column outlet disconnected to avoid the possibility of
contamination of the detector base body.
Column conditioning consists of passing a flow of carrier gas through the
column and heating it to a temperature of 20-50 °C above the maximum
temperature that will be used for running the analysis. For the test column the
working temperature is 350 °C. To perform column conditioning refer to
paragraph
Column Conditioning .
8. Detector Connections
This operation should be carried out at the end of the column conditioning.
Connect the test column to the detector following the instructions reported in: