Thermo Fisher Scientifi c
Thermo Scientifi c Novus SCP/MCP User Manual
19
One point calibration
The one point calibration can be used if a single specifi c volume must be
calibrated. The calibration volume can be selected from the entire volume range.
Note that the inaccuracy of other volumes changes also and the performance
for other volumes cannot be assured.
1. Do the pipetting series with calibration volume.
2. Calculate the results.
3. Press MENU and select options with SCROLLKEY and accept with OK.
4. Select CALIBRATE and press OK.
5. Select one point and confi rm with OK. The calibration volume is shown on
the
display.
6. Press EDIT and change the calibration volume with the SCROLLKEY and
accept with OK.
7. Change the actual volume with the SCROLLKEY and accept with OK.
8. A text SAVE? appears on the display.
9. Accept with YES
10. The adjustment has been changed.
Conversion of mass to volume
V = (w + e) x Z
V = volume (μl)
w = weight (mg)
e = evaporation loss (mg)
Z = conversion factor for μl/mg conversion
Evaporation loss can be signifi cant with low volumes. To determine mass loss,
dispense water to the weighing vessel, note the reading and start a stopwatch.
See how much the reading decreases during 30 seconds (e.g. 6mg = 0.2mg/s).
Compare this to the pipetting time from tareing to reading. Typically pipetting
time might be 10 seconds and the mass loss is 2 mg (10s x 0.2mg/s) in this
example. If an evaporation trap or lid on the vessel is used the correction of
evaporation is usually unnecessary.
The factor Z is for converting the weight of the water to volume at test
temperature and pressure. A typical value is 1.0032μl/mg at 22°C and 95 kPa.
See the conversion table on page 29
.
Formulas for
calculating results
