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Thermo Fisher Scientific

Thermo Scientific Novus SCP/MCP User Manual

   

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Finnpipette

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 Novus

Single Channel & Multichannel

Instructions for Use

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Summary of Contents for Finnpipette Novus

Page 1: ...Thermo Fisher Scientific Thermo Scientific Novus SCP MCP User Manual 1 Finnpipette Novus Single Channel Multichannel Instructions for Use Us er Man ual ...

Page 2: ...position 7 Tip ejection 7 Shelf hanger 7 PIPETTE OPERATION 9 Choosing pipetting functions and speed 9 The Menu Map 10 Pipette Forward technique 10 Rpipet Reverse Repetitive 11 Reverse technique 11 Repetitive technique 11 Stepper multi dispensing 12 Dilute 12 Program 13 Additional functions in program mode 13 Mix Pipette 13 Pipette Count 13 Seq stepper 14 Mix Dilute 14 Manual 15 Seq Aspirate 15 Opt...

Page 3: ...ization Single Channel pipettes 27 Sterilization Multichannel pipettes 27 TROUBLE SHOOTING 28 TECHNICAL DATA 30 SPARE PARTS SCP 31 33 SPARE PARTS MCP 34 37 WEEE Compliance This product is required to comply with the European Union s Waste Electrical Electronic Equipment WEEE Directive 2002 96 EC It is marked with the following symbol Thermo Scientific has contracted with one or more recycling dispo...

Page 4: ... and repetitive pipetting stepper and diluting modes The guiding and easy user interface is very fast to learn The adjusted delivery volume is clearly indicated in the LCD display on top of the handle The long lasting Lithium Ion battery is always charged with rapid charge technique If needed the battery can be charged over the lunch hour Order No Channel Volume Range Finntip 46300000 8 1 µl to 10...

Page 5: ...Bag of grease 1g Order No 3300200 7 Sample Finntips Getting started Remove the content from the package and verify that all items listed above are included Inspect for possible shipping damage Make sure that the pipette is desired volume range and that the voltage of the charger is correct Note If the device is not used according to manufacturers instructions the protection provided by the device ...

Page 6: ... charging the pipette You can recognize the correct power supply by the Thermo Scientific logo and the pipette name on the power supply unit Do not charge the pipette in a hot location 40 C Note If the pipette is supplied with the battery attached the below installation is not needed Check that the battery wires are as shown in the picture Note Do not remove the tape that is holding the wires Slid...

Page 7: ...old battery can cause the pipette to malfunction We recommend that you change the battery every 3 years The index finger operated trigger which activates the piston movement can be adjusted by rotating it 60 degrees to both directions of the center position Usually right handed operators turn it left counter clockwise to get the best possible position for the thumb to eject the tip See picture on p...

Page 8: ... cause personal injury Do not drop the battery Do not expose battery to any kind of mechanical stress or extreme temperatures above 60 C 45 C when charging or under 0 C Warning Do not immerse the unit in liquid and do not clean the upper part of the pipette by spraying Fluid seepage can damage internal components and may cause a safety risk Warning Follow the Novus pipette instructions presented i...

Page 9: ...d a text PRESS TRIGGER appears on display Press the trigger to move the piston to the new initial position Pipetting speeds can be selected with the right selection key whenever the text SPEED is displayed Press SPEED and the speed in starts to blink Select the speed with scroll key and accept with OK Now the speed out starts to blink Select the speed with scroll key and accept with OK Choosing pi...

Page 10: ...rawn in to the tip 2 Wait until the liquid is not moving in the tip and withdraw the tip from the liquid touching it against the edge of the reservoir to remove excess liquid 3 To deliver the liquid press the trigger and hold it down The blow out is also included to empty the tip 4 Release the trigger to return to the ready position If necessary change the tip and continue pipetting The Menu Map P...

Page 11: ...p and this should not be included in the delivery A text BLOWOUT is displayed 4 To empty the tip press trigger again If necessary change the tip and continue pipetting The repetitive technique offers a rapid and simple procedure for repeated delivery of the same volume Fill a clean reagent reservoir with the liquid to be dispensed 1 Dip the tip under the surface of the liquid in the reservoir and ...

Page 12: ...the liquid touching it against the edge of the reservoir to remove excess liquid 3 Deliver the preset volume by pressing the trigger The display shows the number of steps left 4 Continue dispensing by repeating step 3 After last step a text BLOWOUT is displayed 5 To empty the tip completely press trigger and hold it down 6 Release the trigger If necessary change the tip and continue pipetting Note...

Page 13: ... accept with OK Note that there are additional functions available compared to main menu After selecting the function set the volumes speeds etc as described in the function instruction above When working with programs the scroll key selects the program and it is a very fast way to switch between different settings Mix Pipette This function adds automatic mixing after normal pipetting First select...

Page 14: ...pletely press trigger 6 If necessary change the tip and continue pipetting Note The pipette can be emptied at any time by pressing CANCEL left selection key Mix Dilute Select the first pipetting volume by pressing the scroll key up or down Accept the volume with OK Next select the second volume VOL 2 with scroll key and accept with OK Then select the pipetting speeds 1 Dip the tip under the surface...

Page 15: ...hest possible volume is flashing Select the first volume with SCROLLKEY and accept with OK Now Vol 2 appears to display and highest possible volume left is flashing Select the second volume with SCROLLKEY and accept with OK After selecting the last volume the total volume is shown on display and speed in is flashing for selecting the pipetting speeds After selecting the speeds the first volume is shown...

Page 16: ...n on the display when the pipette is in sleep mode To change the default name choose NAME from the menu and press edit The first digit starts to flash Change the digit with SCROLLKEY and accept and move to next digit with OK When the last digit is accepted the name is changed Power With this function the power can be turned off Pressing any key turns on the power Buzzer The buzzer can be turned on a...

Page 17: ...temperature of water pipette and air between 15 C to 30 C The relative humidity must be above 50 Especially with volumes under 50μl the air humidity should be as high as possible to reduce the effect of evaporation loss Special accessories such as the evaporation trap are recommended By selecting MENU OPTIONS CALIBRATE COUNTER the number of pipetttings since last calibration is shown on the displa...

Page 18: ...OLLKEY and accept with OK 8 A text SAVE appears on the display 9 Accept with YES 10 The adjustment has been changed Adjustment Range Volume Inaccuracy Imprecision µl µl s d µl cv 1 10 µl 10 0 120 1 2 0 080 0 8 1 0 120 12 0 080 8 0 5 50 µl 50 0 50 1 0 0 20 0 4 5 0 50 10 0 20 4 0 10 100 µl 100 0 80 0 8 0 30 0 3 10 0 80 8 0 0 30 3 0 30 300 µl 300 4 0 1 3 1 5 0 5 30 4 0 13 1 5 5 0 100 1000 µl 1000 8 0...

Page 19: ...nd accept with OK 8 A text SAVE appears on the display 9 Accept with YES 10 The adjustment has been changed Conversion of mass to volume V w e x Z V volume μl w weight mg e evaporation loss mg Z conversion factor for μl mg conversion Evaporation loss can be significant with low volumes To determine mass loss dispense water to the weighing vessel note the reading and start a stopwatch See how much t...

Page 20: ...e A V0 A inaccuracy mean volume V0 nominal volume Inaccuracy can be expressed as a relative value A 100 x A V0 Imprecision random error Imprecision refers to the repeatability of the pipettings It is expressed as standard deviation s or coefficient of variation cv s standards deviation mean volume n number of measurements Standard deviation can be expressed as a relative value CV CV 100 x S ...

Page 21: ...t snap fitting 2 Push down the ejector button and take the upper ejector top 11 between fingers 3 Release the ejector button to up position but hold the tip ejector top 11 in down position with the fingers 4 Remove the tip cone by pulling it out snap fitting 5 Press and hold the trigger down to drive out the piston 6 Insert the special piston removal tool and pull out the piston 14 The number 2 end is...

Page 22: ...ith the disassembly of the pipette Pipettes up to 1000 µl 1 Remove the lower tip ejector sleeve 13 by pulling it out snap fitting 2 Push down the ejector button and take the upper ejector top 11 between fingers 3 Release the ejector button to up position but hold the tip ejector top 11 in down position with the fingers 4 Remove the tip cone by pulling it out snap fitting 5 Press and hold the trigger d...

Page 23: ... the bigger O ring 19 smaller O ring 20 and O ring support 21 on the piston 3 Slide the small spring 22 on the piston 4 Carefully slide the entire assembly into the tip then push the spring hatch 25 down and turn it 90 degrees 5 Take the upper ejector top 11 between fingers and pull it down 6 Push the tip cone into the handle while holding the ejector top 11 own until you hear a click 7 Reassemble ...

Page 24: ...ervice mode If the pipette is used daily it should be checked and lubricated every three months The servicing procedure starts with choosing the Service mode from the menu Menu Options Service 1 Note that the tip ejector button is in fully up position 2 Pull down the tip ejector part of the module and remove the tip cone module by pulling it out snap fitting 3 Pull out the Colour Ring 15 Open the u...

Page 25: ...rings Take one piston Slide cover ring 32 larger hole support ring 35 30 300μl spring 33 support ring 35 o ring 37 bigger and o ring 36 smaller onto the piston Grease the o ring with the lubricant provided in the pipette package Slide all the parts into the tip cone and close the snap joint of the cover ring 1 10 µl Open the tip cone by carefully releasing the cover ring from its snap joint with t...

Page 26: ...te Novus Pipettes Follow the instructions below according to the battery lid version Opening the Version 1 battery lid Use a small flat screwdriver for opening the battery lid Put the head of the screwdriver into the slot and rotate it to open the lid Disconnect the battery connector and pull out the battery Opening the Version 2 battery lid Open the battery lid by pressing and pulling the lid as s...

Page 27: ...Take out the spring 16 8 Autoclave all module parts at 121 C 252 F for 20 minutes 9 Let the parts cool down to room temperature for at least two hours 10 With pipettes up to 100 μl reassemble the plate 25 and spring 16 With pipettes 300 μl and 1000 μl reassemble the spring 16 11 Attach the module back to the pipette as described in Maintenance section After autoclaving the module must be cooled to...

Page 28: ...tion Attach firmly Clean tip cones attach new tips Clean and grease O ring and cylinder Grease accordingly Change the O ring Follow instructions carefully Attach firmly Recalibrate according to instructions Recalibrate with the liquids in question Remove tip cone module Move piston by hand or with piston removal tool Attach the module in service mode TROUBLE SHOOTING The table below lists possible p...

Page 29: ...1 0028 1 0029 1 0029 1 0030 1 0030 21 0 1 0028 1 0029 1 0029 1 0030 1 0031 1 0031 1 0031 21 5 1 0030 1 0030 1 0031 1 0031 1 0032 1 0032 1 0032 22 0 1 0031 1 0031 1 0032 1 0032 1 0033 1 0033 1 0033 22 5 1 0032 1 0032 1 0033 1 0033 1 0034 1 0034 1 0034 23 0 1 0033 1 0033 1 0034 1 0034 1 0035 1 0035 1 0036 23 5 1 0034 1 0035 1 0035 1 0036 1 0036 1 0036 1 0037 24 0 1 0035 1 0036 1 0036 1 0037 1 0037 1...

Page 30: ... Output voltage 5 0 V 0 5 A Finnpipette Novus Type Weight with battery 148 Single channel pipette 210 g max tip not included Operation Altitude Multichannel pipette 315 g max Indoor use only Up to 2000 m Operating temperature 15 to 35 C Air humidity during operation 20 to 85 rel humidity Storage temperature 1 month 20 to 45 C 60 25 Rel humidity 1 month 10 to 35 C 60 25 Rel humidity It is recommend...

Page 31: ... 2209600 13 1033050 14 1060510 15 1060530 0 5 5ml 2209570 1 2209670 10 1062610 11 1132390 12 2209590 13 1030230 14 1060790 15 1060810 100 1000μl 2209560 1 2209660 10 1062030 11 1062600 12 1132180 13 1062060 14 1062360 16 1132620 17 1060630 20 1030020 25 1061350 1 10 ml 1 10 12 13 14 15 10 11 14 20 10 12 13 17 12 13 14 15 11 11 25 16 0 5 5 ml 100 1000 μl ...

Page 32: ...er Manual Thermo Fisher Scientific 30 300 μl 10 100 μl 5 50 μl 5 50 μl Micro 1 10 μl 1 10 μl Micro 14 20 10 12 13 17 11 25 16 14 20 10 12 13 17 11 25 16 14 26 10 12 13 17 11 25 16 14 24 10 12 13 17 11 25 16 22 21 20 19 26 22 21 20 19 18 23 ...

Page 33: ...6 1061410 30 300μl 2209550 1 2209650 10 1062020 11 1062590 12 1132180 13 1062050 14 1062250 16 1132620 17 1060620 20 1033180 25 1061430 10 100μl 2209540 1 2209640 10 1062020 11 1062570 12 1132180 13 1062050 14 1062260 16 1132620 17 1060610 20 1030510 25 1061420 1 10μl 2209510 1 10μl Micro 2209500 1 2209690 1 2209620 Micro 10 1062000 11 1062570 11 1062560 Micro 12 1132180 13 1062050 13 1062040 Micr...

Page 34: ...0 14 1062470 15 1062460 16 1062450 17 1062380 18 1062390 19 1132470 21 4x0202040 22 1132490 23 2x1062401 31 2x11072010 32 10593840 33 2x1132130 34 1132150 35 2x10593500 36 2x1030170 37 10593870 42 2207950 8 pcs 1 10μl 1 2209760 31 2209870 32 1061020 34 1131630 35 10593280 36 1030380 37 1033160 38 10593290 39 1131900 40 10593360 41 1030170 42 2209110 12 pcs 5 50μl 1 2209770 31 2208880 32 1061020 33...

Page 35: ...er Manual 35 1 10μl 8 ch 2209800 1 10μl 12 ch 2209810 5 50μl 8 ch 2209820 5 50μl 12 ch 2209830 1 10μl 5 50μl 15 14 18 19 40 32 17 16 39 31 35 34 36 37 38 41 30 42 21 21 22 23 24 32 31 35 33 36 37 30 42 34 Picture Abbildung Illustration Imagen Bild Immagine 図 ...

Page 36: ...P MCP User Manual Thermo Fisher Scientific 5 50μl 16 ch 2209860 30 300μl 8 ch 2209840 30 300μl 12 ch 2209850 32 31 35 33 36 37 30 34 42 15 14 18 19 17 16 21 21 22 23 20 35 21 21 22 23 24 32 31 35 33 36 30 34 42 15 14 18 19 17 16 37 ...

Page 37: ...fic Novus SCP MCP User Manual 37 1200 μl 8 channel 1 2209940 14 1062470 15 1062460 16 1062780 17 1062750 18 1062740 19 1132480 21 4x0202050 22 1132630 23 2x1062760 30 1062720 31 2209920 1200μl 8 ch 2209930 31 30 21 21 22 23 15 14 18 19 17 16 ...

Page 38: ...o 20 Univ 30 Flex 30 Univ 50 Micro 100 Flex 100 Ext 100 Univ 200 Flex 200 Ext 200 Univ 300 Flex 300 1000 Ext 1000 1000 Flex 1200 Flex 5 ml 10 ml 10 ml Flex Ext FP Novus 1 10μl Micro FP Novus 1 10μl FP Novus 5 50μl Micro FP Novus 5 50μl FP Novus 10 100μl FP Novus 30 300μl FP Novus 100 1000 μl FP Novus 0 5 5ml FP Novus 1 10ml FP Novus 8 ch 1 10μl FP Novus 12 ch 1 10μl FP Novus 8 ch 5 50μl FP Novus 1...

Page 39: ...rack 9402073 5 ml sterile 1 5 ml 5x54 rack 9402160 10 ml 1 10 ml 5x24 rack 9402163 10 ml sterile 1 10 ml 5x24 rack 94060970 Flex 10ml Ext 1 10 ml 100 pcs bag 94060973 Flex 10ml Ext sterile 1 10 ml 50 pcs bag Also available in bags Code Finntip Filter Volume Qty 94052000 10 Micro sterile 0 2 10 μl 10x96 rack 94056980 Flex 10 sterile 0 2 10 μl 10x96 rack 94052100 10 sterile 0 5 10 μl 10x96 rack 9405...

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