Thermo Scientific Dionex AutoTrace 280, Operator'S Manual

Page: 70 / 166

Dionex AutoTrace 280 Operator’s Manual
62
Doc. 065330-05 03/2020
c. Press
CONT
once to select method 29.
NOTE To use a customized priming method, substitute
the number of that method in the instructions
above.
5. Press CONT to run the method. The method draws enough solvent
from each port to prime the liquid lines.
6. When the system pauses, press CONT .
3.7.8 Flushing the Sample Lines
For instructions on how to flush the sample lines, see Section 3.7.6 .
3.8 Method Development Checklist
3.8.1 Specifying Your Method
•
Run the AutoTrace software.
•
Specify the parameter and solvent information.
•
Create the method.
•
Save and print the method.
3.8.2 Preparing the System
•
Turn on the system.
•
Turn on and check the gas supply. Set the gauge, if applicable.
•
Check the solvent reservoirs and fill as needed.
•
Check the waste containers and empty as needed.
•
Prime the solvent lines.
•
Load the elution rack.
•
For the 40 mL elution rack only: Check the alignment of the elution
nozzles. If a nozzle touches the side of the collection vial, align it (see
Section 5.18 ).
•
(Optional) Clean the SPE cartridges or disks.