Cryogenic Storage Vessel
4-1
Thermo Scientific
Section 4
Operation
Caution
Never overfill your Locator or Locator Plus vessel with liquid
nitrogen. The liquid nitrogen level in your tank (with racks inserted)
should never be above 20 inches (50cm) for Locator 4 and Locator 8, 10
inches (25cm) for Locator Jr., 22 inches (55.8cm) for Locator 4, 6, and 8
Plus and 12 inches for Locator Jr. Plus. Filling the tank up to or above the
bottom of the necktube may cause immediate or premature vacuum failure
to occur.
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Caution
When inserting or removing racks, be careful not to come in
contact with the necktube area of the vessel. Remove or insert racks slowly
in a vertical manner. Scratches on the necktube area can cause premature
vacuum failure.
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Caution
Do not spill liquid nitrogen on vacuum port (covered by a black
plastic cap on side of vessel). This can cause vacuum failure.
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To avoid damage to your Locator or Locator Plus cryogenic storage vessel
which may result in premature vacuum loss, it is important that the
following procedure be used when adding liquid nitrogen to a warm vessel.
1. Add only a small amount of liquid nitrogen (5-10 liters) to a new or
warm vessel.
2. Allow this small amount of liquid nitrogen to sit in the covered vessel
for a minimum of 2 hours. This will limit stress caused by the sudden
temperature change associated with adding liquid nitrogen to a warm
vessel.
3. Add an additional 15 liters of liquid nitrogen to the vessel.
4. Allow vessel to sit for 48 hours and monitor liquid nitrogen
consumption with a wooden yarsdstick, optional dip measurer (part
#180143) or Level Monitor.
5. Fill Locator or Locator Plus as indicated (see Caution in this section).
Allow for displacement of liquid nitrogen when racks and boxes are
inserted.
6. Insert and remove racks slowly. Allow liquid nitrogen to run out of
boxes and off racks.
Filling Instructions
