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Thermo Scientific

14  |  ALPS30

Table 4: Recommended Film and Foil Types

Description

Compatible  

Plate 

Material

Sealing 

Conditions

Seal 

Integrity 

Range

Applications

Films

Clear Seal

PP, PS, PE

1.5 to 2.5 
seconds 
at 165 to 
170°C

-200 to 
90°C 
and up 
to 120°C 
with cycler 
pressure

Suitable for fluorescence and 
colorimetric applications; 
suitable for PCR applications 
with screw-down or clip-down 
heated lid thermal cyclers only

Clear Seal 
Diamond

PP, PS, PE, 
COC

1.5 to 3.0 
seconds at 
170°C

-80 to 
120°C

Ideal for fluorescence and 
colorimetric applications; 
suitable for PCR applications 
with screw-down or clip-down 
heated lid thermal cyclers only

Clear Seal 
Strong

PP

1.5 to 2.0 
seconds at 
170°C

-80 to 
120°C

Clear for sample inspection; 
due to strong, unpeelable seal 
properties, it is suitable for stor-
age and disposal of  hazardous 
material; also suitable for PCR 
applications

Foils

Thermo-Seal

PP

1.5 to 2.5 
seconds at
165 to 
170°C

-80 to 
120°C

Long term storage including 
storage at low temperatures; 
transportation and high 
temperature applications such 
as PCR, including use in water 
bath thermal cyclers

Easy Pierce

PP, PE

0.5 to 3.0 
seconds 
at 165 to 
175°C

-80 to 
80°C 
and up to 
120°C with 
cycler lid 
pressure

Formats where piercing is a 
useful method for sample re-
trieval; second seal can be ap-
plied over existing seal; suitable 
for PCR with screw-down lid

Easy Pierce 
20 μm

PP, PE

0.5 to 2.0 
seconds 
at 165 to 
175°C

-80 to 
80°C 
and up to 
120°C with 
cycler lid 
pressure

Well suited for 384-well 
formats where piercing is a 
useful method for sample 
retrieval; suitable for PCR with 
screw-down lid

Easy Peel

PP, PE, COC

1.5 to 2.5 
seconds 
at 165 to 
170°C

-200 to 
90°C and
up to 
120°C 
with cycler 
pressure

Ideal for long term storage at 
low temperatures; seal can be 
applied, removed and a new 
seal applied several times; suit-
able for PCR with screw-down 
heated lid thermal cyclers only

Chapter 4: Unit Description

Summary of Contents for ALPS30

Page 1: ...U s er M an u al Thermo Scientific ALPS30 Manual Heat Sealer May 2015 ...

Page 2: ...This page intentionally left blank ...

Page 3: ...ts Symbols Used in Manual 4 Safety Precautions and Limitations of Use 5 Regulatory Limitations of Use 9 Unit Description 11 Description 11 Intended Use 11 Components 12 Unit Installation 15 Unit Operation 18 Status Indicator LEDs 18 Performing a Sealing Operation 19 Maintenance and Servicing 21 Replacing the Unit Fuse 22 Over Temperature Safety Cutout 22 Routine Cleaning and Inspection 23 Decontam...

Page 4: ...ndicates a Risk of Explosion which could if not avoided result in severe injury or death WARNING Indicates a hazardous situation which could if not avoided result in severe injury or death or severely damage the unit CAUTION Indicates a hazardous situation which could if not avoided result in minor or moderate injury or degrade or impair the functionality of the unit CAUTION Indicates a Risk of Cr...

Page 5: ...zards associated with the unit and its accessories All operators should be familiar with the safety precautions and warnings given in these instructions before attempting to operate the unit Improper use of this unit or its accessories may impair their function ality and invalidate the manufacturer s warranty Unit Handling Precautions CAUTION Care should be taken not to drop the unit or subject it...

Page 6: ...kage and over current protection WARNING Always ensure that the mains power connector is securely inserted into the rear of the unit and any excess power cord does not pose a potential trip or pull hazard DANGER Do not operate the unit in any area which is has been or is thought to have been exposed to explosive or flammable gases vapors or liquids WARNING The unit must be installed and operated o...

Page 7: ...USE will only blow under a fault condition This FUSE should only be changed after the unit has been thoroughly inspected by a qualified technician See Replacing the Unit Fuse for details DANGER Always switch off the unit and disconnect the power cord before performing any cleaning or decontamination procedure If liquid is spilled into or over the unit switch off the unit and discon nect the power ...

Page 8: ...Thermo Scientific 8 ALPS30 Unit Maintenance and Serviceability CAUTION Do not autoclave any part of the unit or its accessories Chapter 2 Safety Precautions and Limitations of Use ...

Page 9: ...lled and operated in accor dance with the instructions in this manual The ALPS30 unit has been type tested by Trac Global UKAS approved test facility and UK appointed Notified Body against the EMC Requirements listed below and issued Certificate No 6660 10 and 6661 10 Safety and EMC Requirements SAFETY EN 61010 1 2010 EN 61010 2 051 2003 UL 61010 1 2001 2nd Edition CAN C22 2 CSA 61010 1 EMC EN 613...

Page 10: ...ropean Union WEEE Directive 2002 96 EC on Waste Electrical and Electronic Equipment Do not dispose of this product into unsorted municipal waste or public landfill Please refer to Product Disposal section for details of how to correctly dispose of this product The ALPS30 unit is designed and manufactured under ISO 9001 by Thermo Fisher Scientific 75 Panorama Creek Drive Rochester NY 14625 1 585 58...

Page 11: ...ation and cross contamination between wells The Heat Sealer is designed to provide sealing consistency in low throughput labs Heat sealing offers superior sample protection of storage and reaction plates in applications including compound storage sample archiving and Polymerase Chain Reaction PCR The ALPS30 Heat Sealer is compatible with virtually any height microplate or block including PCR plate...

Page 12: ...Activation Handle 2 Status Indicator LEDs See Status Indicator LEDs 3 Heater Plate Internal 4 Adapter Plate Carrier 5 Power Inlet On Off Switch and Fuse Holder See Unit Installation 6 Mains Voltage Selector See Unit Installation 7 Product Information Label Chapter 4 Unit Description 1 2 3 4 5 6 7 Figure 1 Unit Components and Features ...

Page 13: ...Blocks Suitable Plate Types 96 Well Microtiter Plate 384 Well Microtiter Plate 96 Well Skirted PCR Plate 96 Well Semi skirted PCR Plate 96 Well Unskirted PCR Plate Deepwell Plates Plate Material Types Polypropylene Polyethylene or Polystyrene Other specialist ADAPTER BLOCKS may be available on request Please contact your distributor for details A comprehensive range of Thermo Scientific clear film...

Page 14: ...PCR applications Foils Thermo Seal PP 1 5 to 2 5 seconds at 165 to 170 C 80 to 120 C Long term storage including storage at low temperatures transportation and high temperature applications such as PCR including use in water bath thermal cyclers Easy Pierce PP PE 0 5 to 3 0 seconds at 165 to 175 C 80 to 80 C and up to 120 C with cycler lid pressure Formats where piercing is a useful method for sam...

Page 15: ...rements Safe and suitable operating environment see Safety Precautions and Limitations of Use Solid stable and level working surface At least 4 in 10 cm clearance around the unit to adjacent objects and walls Grounded AC mains power connection see Technical Specifications WARNING Please observe and abide by the Unit Installation and Operating Environment safety precautions and preconditions listed...

Page 16: ...surface as specified above 2 Connect the unit to the AC mains power outlet using the mains power cord supplied or as specified in Technical Specifications 3 Switch the mains power on at the supply outlet first and then switch the unit on using the POWER SWITCH located at the rear of the unit See Components for POWER SWITCH location 4 Verify the unit is stable and safe by performing a trial sealing...

Page 17: ...ific ALPS30 17 DANGER If the unit has been stored in a cool environment it must be left to stand unplugged until it has acclimatized to the new room temperature before connecting to power Chapter 5 Unit Installation ...

Page 18: ... Indicator LEDs Table 6 Status Indicator LEDs Amber HEATER Indicator On HEATER PLATE is warming up Off HEATER PLATE is cooling down Flashing HEATER PLATE at 340ºF 5ºF 170ºC 2ºC Green POWER Indicator On Unit switched on Off Unit switched off or no power When the unit is switched on the green POWER LED will illuminate and the unit will automatically start heating to 340ºF 170ºC This is indicated by ...

Page 19: ...the rear of the unit and allow sufficient time for it to reach temperature the HEATER LED will start flashing 2 Using a suitable ADAPTER BLOCK listed inTable 3 Plate and Adapter Block Types load the sample plate onto the PLATE CARRIER and add the sealing film on top right side up DANGER Care should be taken not to touch the surface of the HEATER PLATE while loading the sample plate 3 Grasp the HAN...

Page 20: ...sonal protective equipment PPE remove the sealed plate from the unit and review the seal integrity WARNING The sample plate and film foil may remain hot for several seconds after being removed from the unit The time taken to successfully seal a sample plate may vary with both plate and film foil types For optimum seal integrity the sealing time may need to be adjusted empirically to achieve the de...

Page 21: ...ease observe and comply with all of the Unit Maintenance and Serviceability precautions listed in Safety Precautions and Limitations of Use Never remove the unit casework There are no user or operator service able parts inside the unit Always switch off and unplug the unit before performing any cleaning or disinfecting tasks For technical and service related inquiries please contact your distribut...

Page 22: ...ing a small flat bladed screwdriver to carefully unclip and pull open the FUSE HOLDER see Unit Installation Verify that the new fuse is the correct one for the operating voltage being used see Table 5 Mains Voltage Selection After replacing the fuse push the FUSE HOLDER firmly back into the POWER INLET MODULE The unit must be safety tested for excess current leakage before being repowered from the...

Page 23: ... cleaning always inspect the unit casework HEATER PLATE and all moving parts for any signs of wear damage cracks or other defects 3 Wearing suitable PPE clean the casework using a damp cloth soaked with a disinfectant solution such as Virkon 4 Carefully clean the HEATER PLATE surface to remove any debris or sealing material 5 Remove any debris from around or between the moving parts of the handle ...

Page 24: ...Thermo Scientific 24 ALPS30 Decontamination Procedure The unit and accessories must be decontaminated using the following procedure before being stored or transported Chapter 7 Maintenance and Servicing ...

Page 25: ...ked Product Disposal At end of life this product must be disposed of in accordance with your local authority regulations for the disposal of potentially hazardous waste and electronic equipment The unit and its accessories must be decontaminated using the Decontamination Procedure before disposal or shipping Do not dispose of this product into unsorted municipal waste or public landfill Do not dis...

Page 26: ... adapter or peripheral not supplied specified or sanctioned by Thermo Fisher Scientific will invalidate this warranty This warranty is provided to the original purchaser of the product for one year from the date of purchase Under the terms of this warranty the product must be returned in its original packaging transportation prepaid by the sender with a copy of the Proof of Purchase and a detailed...

Page 27: ...wing North America Thermo Fisher Scientific 75 Panorama Creek Drive Rochester NY 14625 Tel 585 586 8800 1 800 625 4327 Email technicalsupport thermofisher com Europe UK Thermo Fisher Scientific Robert Bosch Straße 1 63505 Langenselbold Germany Tel 49 0 6184 906 000 Email orders labequipment de thermofisher com 9 ...

Page 28: ...wer Consumption Fuse Ratings and Sizes Operating Environment Temperature Range Relative Humidity Range Maximum Operating Altitude ALPS30 Manual Heat Sealer AB 0400 8 7 x 9 8 x 9 1 in 220 x 250 x 230 mm 8 8 lb 4 kg IEC C13 3 Core 5A min IEC C14 DPST Single Fuse 115 110 to 120 V AC 10 230 220 to 230 V AC 10 50 to 60 Hz 5 350 W max 115 T3 15AH 250V 20 x 5 mm 230 T1 6AH 250V 20 x 5 mm 65 to 85ºF 18 to...

Page 29: ... ALPS30 29 15 to 120ºF 10 to 50ºC 20 to 95 non condensing 340ºF 170ºC Non adjustable 2ºC Storage and Transportation Temperature Range Relative Humidity Range Sealing Parameters Temperature Set point Temperature Accuracy ...

Page 30: ...l Plate EMC Electromagnetic Compatibility Microtiter Plate MTP Plate with an SBS footprint featuring 24 48 96 or 384 wells MTP Microtiter Plate PCR Polymerase Chain Reaction PPE Personal Protective Equipment SBS Society for Biomolecular Screening Semi skirted PCR Plate PCR plate with an outer surrounding half edge Skirted PCR Plate PCR plate with an outer surrounding edge Un skirted PCR Plate PCR ...

Page 31: ...Thermo Scientific ALPS30 31 Notes ...

Page 32: ...r Scientific and its subsidiaries Specifications terms and pricing are subject to change Not all products are available in all countries Please consult your local sales representa tive for details Virkon is a registered trademark of DuPont Thermo Fisher Scientific 75 Panorama Creek Drive Rochester NY 14625 MNLSPALPS30 0515 ...

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