10
Step 6:
Reconnecting
the Pump to the
LC System
Y
To reconnect the pump to the LC system
1. Reconnect the solvent lines to the vacuum degasser inlets.
2. To connect the pump to the autosampler, do one of the following:
• Using high-pressure fittings, connect a 20-cm length of stainless steel tubing between the mixer outlet and
the column oven inlet of the autosampler.
• Using high-pressure fittings, connect a 20-cm length of stainless steel tubing between the in-line filter outlet
and the column oven inlet of the autosampler or port 5 of the autosampler injection valve.
3. Pump mobile phase through the system to remove air.
Trademarks
Accela is a trademark of Thermo Fisher Scientific Inc.
Tip
To decrease the system gradient delay volume, you can bypass the mixer for mobile phases that do
not require additional mixing.
