Thermo Scientific 7450 Operating And Maintenance Manual Download Page 12

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Pre-Set Profile #3

- Commonly used for straw size samples, resulting in a

slow rate to -7°C, a 5 minute hold for manual cell nucleation, a 0.3°C
rate to -35°C, and then a 0.1°C per minute cooling rate to a -37°C end
temperature.

Step 1 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Wait

at 20C

Step 2 . . . . . . . . . . . . . . . . . . . . . . . . . . . .1.0 C/m C

to -4.0C

Step 3 . . . . . . . . . . . . . . . . . . . . . . . . . . . .0.5 C/m C

to -7.0C

Step 4 . . . . . . . . . . . . . . . . . . . . . . . . . . .Hold for 5m

at -7.0C

Step 5 . . . . . . . . . . . . . . . . . . . . . . . . . . . .0.3 C/m C

to -35C

Step 6 . . . . . . . . . . . . . . . . . . . . . . . . . . . .0.1 C/m C

to -37C

Step 7 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .End

Pre-Set Profile #4

- Commonly used for hemopoietic stem cells that are

65-100ml in size, resulting in a 1°C rate from nucleation to -45°C and a
10°C per minute cooling rate to a -90°C end temperature.

Step 1 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Wait

at 20.0C

Step 2 . . . . . . . . . . . . . . . . . . . . . . . . . . . .1.0 C/m S

to -6.0C

Step 3 . . . . . . . . . . . . . . . . . . . . . . . . . . .25.0 C/m C

to -50C

Step 4 . . . . . . . . . . . . . . . . . . . . . . . . . . .10.0 C/m C

to -14.0C

Step 5 . . . . . . . . . . . . . . . . . . . . . . . . . . . .1.0 C/m C

to -45C

Step 6 . . . . . . . . . . . . . . . . . . . . . . . . . . .10.0 C/m C

to -90C

Step 7 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .End

Pre-Set Profile #5

- Commonly used for skin packets, resulting in a 1°C

rate from nucleation to -35°C and a 10°C per minute cooling rate to a
-90°C end temperature.

Step 1 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Wait

at 4.0C

Step 2 . . . . . . . . . . . . . . . . . . . . . . . . . . . .1.0 C/m S

to -5.0C

Step 3 . . . . . . . . . . . . . . . . . . . . . . . . . . .20.0 C/m C

to -40C

Step 4 . . . . . . . . . . . . . . . . . . . . . . . . . . .10.0 C/m C

to -10.0C

Step 5 . . . . . . . . . . . . . . . . . . . . . . . . . . . .1.0 C/m C

to -35C

Step 6 . . . . . . . . . . . . . . . . . . . . . . . . . . .10.0 C/m C

to -90C

Step 7 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .End

7007452 CryoMed Freezer 1-5

Thermo Scientific

Section 1

Introduction

Pre-set Profiles

(continued)

Summary of Contents for 7450

Page 1: ...Model 7452 Series CryoMed Controlled Rate Freezer Operating and Maintenance Manual 7007452 Rev 11 ...

Page 2: ...r IVF units ccs 20558 FR 1582 3 18 02 Updated schematics and wiring diagrams for external IVF connector probe ccs 2 20604 FR 1592 3 12 02 Updated schematics and wiring diagrams aks 12 21 01 Added IVF models ccs 1 11 12 01 Updated profiles ccs 20382 FR1555 11 13 01 Change hinge mounting screws for freezer door aks 0 200164 SI 8280 8 23 01 Original manual ccs REV ECR ECN DATE DESCRIPTION By Preface ...

Page 3: ... All internal adjustments and maintenance must be performed by qualified service personnel Material in this manual is for information purposes only The contents and the product it describes are subject to change without notice Thermo Scientific makes no representations or warranties with respect to this manual In no event shall Thermo be held liable for any damages direct or incidental arising out...

Page 4: ...002 96 EC WEEE and the equipment that has been put on the market after 13 August 2005 This product is required to comply with the European Union s Waste Electrical Electronic Equipment WEEE Directive 2002 96 EC It is marked with the WEEE symbol Thermo Scientific has contracted with one or more recycling disposal companies in each EU Member State European Country and this product should be disposed...

Page 5: ... spare or replacement parts or provide you with on site service We can also provide you with a quotation on our Extended Warranty for your Thermo Scientific products Whatever Thermo Scientific products you need or use we will be happy to discuss your applications If you are experiencing technical problems working together we will help you locate the problem and chances are correct it yourself over...

Page 6: ...ss Code Security 2 5 Initiate a Profile 2 5 Stop a Profile Run 2 6 Manual Override 2 7 Program User Specific Profiles 2 7 Copy and Paste Pre set and User Profiles 2 10 Recorder 2 11 Printer 2 11 Alarms 2 12 Chamber Thermocouple Open 2 12 Sample Thermocouple Open 2 13 Heater Malfunction 2 14 Chamber Sample Over Under Limit Temp 2 15 Power Failure 2 17 Run Complete 2 17 Warm Complete 2 18 Seeding Ho...

Page 7: ...r Profile 10 7 Edit an Existing Profile 10 8 Print a Profile Listing 10 8 Display Profile Temp Graph 10 9 Error Messages 10 9 Run Screen 10 10 User Control Definitions 10 10 Status Indicators 10 13 Operating Indicators 10 13 Typical Run Procedure 10 15 Power Failure Recovery 10 16 Error Messages 10 17 Display Control Summary 10 18 Remote Mode 10 18 Stand Alone Mode 10 19 Running a Local Profile 10...

Page 8: ...7007452 CryoMed Freezer 1 1 Thermo Scientific Section 1 Introduction Figure 1 1 Non IVF Controlled Rate Freezer Figure 1 2 IVF Controlled Rate Freezer FDA clearance for human research ...

Page 9: ...tion to prevent ice build up The IVF model of the Controlled Rate Freezer has top mounted sample access ports used to seed the samples during the freezing process Seeding should always be performed through the access ports rather than the door to reduce sample temperature fluctuations during the seeding process There are three displays on the Controlled Rate Freezer control panel Chamber and Sampl...

Page 10: ...dible alarm and clears the alarm messages from the display Back Terminates a profile in progress returns to Program menu or moves the cursor to the left Enter Starts a new line enters a sub menu moves the cursor to the right or enters View or Edit Mode Up Scrolls upward through display lines or changes profile step and set up parameters Down Scrolls downward through display lines or changes profil...

Page 11: ...n 3 User specific profiles can also be entered and saved See Section 2 Pre Set Profile 1 Commonly used for 2 0ml sample size resulting in a 1 C rate from nucleation to 40 C and a 10 C per minute cooling rate to a 90 C end temperature Step 1 Wait at 4 0C Step 2 1 0 C m S to 4 0C Step 3 25 0 C m C to 40C Step 4 10 0 C m C to 12 0C Step 5 1 0 C m C to 40C Step 6 10 0 C m C to 90C Step 7 End Pre Set P...

Page 12: ... in a 1 C rate from nucleation to 45 C and a 10 C per minute cooling rate to a 90 C end temperature Step 1 Wait at 20 0C Step 2 1 0 C m S to 6 0C Step 3 25 0 C m C to 50C Step 4 10 0 C m C to 14 0C Step 5 1 0 C m C to 45C Step 6 10 0 C m C to 90C Step 7 End Pre Set Profile 5 Commonly used for skin packets resulting in a 1 C rate from nucleation to 35 C and a 10 C per minute cooling rate to a 90 C ...

Page 13: ...ng rate to a 80 C end temperature Step 1 Wait at 4 0C Step 2 1 0 C m S to 4 0C Step 3 20 0 C m C to 45C Step 4 10 0 C m C to 10 0C Step 5 0 5 C m C to 20C Step 6 1 0 C m S to 80C Step 7 End S Sample Temperature C Chamber Temperature C m degrees Centigrade per Minute 1 6 7007452 CryoMed Freezer Thermo Scientific Section 1 Introduction Pre set Profiles continued ...

Page 14: ...in unconsciousness and death Therefore the use of oxygen monitoring equipment is strongly recommended Warning Contact of liquid nitrogen with the skin or eyes may cause serious freezing injury This freezer should be located in a well ventilated area of the laboratory on a table bench capable of supporting the unit s weight see Specifications section with adequate workspace available Allow clearanc...

Page 15: ...2 2 7007452 CryoMed Freezer Thermo Scientific Section 2 Set Up and Operation Figure 2 2 Clearance for All Units ...

Page 16: ...Freezer is designed to be electrically safe in the fol lowing environmental conditions Indoors Altitude Up to 2 000 meters Temperature 5 C to 40 C Humidity 80 RH at or below 31 C decreasing linearly to 50 RH at 40 C Mains Supply Fluctuations 10 of nominal Installation Category II 1 Pollution Degree 2 2 Class of Equipment I 1 Installation category overvoltage category defines the level of transient...

Page 17: ...h the internal thermocouple connector Refer to Section 3 for probe calibration 1 Turn the freezer power switch on the back of the unit to On 2 On the control panel the Alarm indicator lights briefly and the LCD display reads Controlled Rate Freezer XX XX software version 3 The Idle indicator lights then the LCD display shows the profile list 4 To choose a profile scroll through the selections usin...

Page 18: ...nto the selected protected function or menu 2 The access code when protection is disabled is 0 0 0 0 3 The access code can be enabled changed or disabled by using Change Access Code see Configuration Calibration section 1 Load the product into the freezing chamber On IVF models make sure all unused sample access ports are capped 2 If the profile list is not on the display press Back until the list...

Page 19: ...ve the control will automatically exit the the move mode and continue profile execution without interruption If Access Code protection has been enabled see Configuration Calibration section the unit will prompt for the access code before moving to the selected profile step A profile run can be discontinued by pressing the Back key If Access Code protection has been enabled the unit will prompt for...

Page 20: ...l For example if the Cool function is activated under manual override and the chamber drops below the programmed setpoint the controller will activate the Heat function to compensate To program user specific profiles enter Edit Mode The following describes the actions and options necessary for adding or editing a profile Note If Access Code protection has been enabled the unit will prompt for the ...

Page 21: ...the cursor over the chosen character and press Silence Note When starting with a space Sp pressing the up arrow key gives you a while pressing the down arrow key gives you a 5 At this time enter a unique name or title for the profile 6 When Step 0 the name or title is complete move the cursor back to the leftmost position This is View Mode 7 Scroll using the down arrow key to Step 1 8 Press Enter ...

Page 22: ... by plac ing the cursor on the S or C and using the up or down arrow keys Caution Steep ramp rates may cause chamber to be much colder warmer than the sample itself Thermal controllers are limited to 55 C and 185 C during steep Sample ramps Note For chamber ramp steps the interior set temperature will ramp 1 C beyond the programmed temperature to assure that the ramp end point condition will be re...

Page 23: ...device See Section 3 Note To edit a single line of a profile enter View Mode scroll to the desired profile step and go to Edit Mode by pressing Enter Pre set and created user profiles can be copied then pasted into one of the ten User Profiles as templates to create a new User Profile To erase a User Profile already in use a blank User Profile may be copied then pasted over it Use the steps below ...

Page 24: ...ich might have occurred Run data is captured in a printer buffer for later reprinting even if the printer is not enabled during the Run The printer buffer is cleared at the start of each new run It is recommended that a supply of printer paper be kept on hand If paper should run out during a freezing run first install the paper Then when the Run is complete the print out may be re started 7007452 ...

Page 25: ...ber thermocouple cannot be detected Audible alarm sounds continuously Alarm indicator lights Message C sensor fail shows on the LCD display ILL shows on the numeric display chamber Freezer switches to Idle Mode and operating functions are disabled The audible alarm is silenced by the first depression of the Silence key When the alarm condition has cleared another depression of the Silence key is n...

Page 26: ... using the Sample temperature for a trip point Audible alarm sounds Run start prevented LCD Display shows S sensor fail on the fourth line alternated with S sensor req The Alarm LED and display messages remain active until the probe condition is resolved 2 Run Mode If the Sample probe fails during Run Mode and the current profile uses the Sample temperature as a trip point Alarm LED lights Audible...

Page 27: ...If the heater is energized continuously no active temperature control for 60 minutes the controller will sound a heater malfunction alarm light the Alarm LED sound the audible alarm continuously show the message Heater error on the fourth line of the display and immediately switch to Idle Mode and disable all functions The audible alarm is silenced by pressing the Silence key Since this alarm indi...

Page 28: ... second rate 5 Continue the profile run without interruption This is a self clearing alarm While in the alarm condition the audible alarm can be silenced by one depression of the Silence key but the Alarm LED continues to flash and the alarm message displays as long as the alarm condition is present When the chamber temperature returns to a value within the pre set limits the Alarm LED stops flash...

Page 29: ...recorder and thermal printer at 50 C to document on paper where the alarm occurred Continue the profile run without interruption This is a self clearing alarm When in the alarm condition the audible alarm can be silenced by one depression of the Silence key but the Alarm LED will continue to flash and the alarm message be displayed while the alarm condition is present When the alarm condition is r...

Page 30: ...ntinue profile run without interruption A single depression of the Silence key will clear the audible alarm A second depression of the Silence key will clear the Alarm LED and the messages on the second third and fourth display lines Five minutes after the End profile step is reached the controller will sound the Run Complete alarm Flash Alarm LED and audible tone at a 25 On duty cycle 2 second pe...

Page 31: ...ss ports rather than the door This alarm message appears when the printer storage buffer is full of Run data approximately 2 hours and is no longer able to store data for later printing Press Silence to mute the alarm However the LED will continue to flash until the Run is stopped Press Silence to clear the alarm message Between the time the Printer Buffer Full messsage displays and the user stops...

Page 32: ...ernal connection on the top of the unit See Figure 1 2 The straw sample holder secures up to 4 straws for insertion through the access port on the top of the IVF freezer The straw holder may also be used to hold the external sample probe See Figure 2 6 1 To secure the straws first make sure the holder is in an upright vertical position 2 Press the button on the top of the holder This temporarily w...

Page 33: ...e plastic handle Insert the cane holder assembly through the access port on the top of the unit See Figure 1 2 If the cane holder is used with a sample probe use the standard 1 2ml vial probe Connect the probe to the internal sample probe connection Caution Do not connect two sample probes at the same time 2 20 7007452 CryoMed Freezer Thermo Scientific Section 2 Set Up and Operation Using the Stra...

Page 34: ...m longer widen the opening or to the left to make them shorter narrow the opening Note the threads at the bottom of the adjustment rods 3 Tighten the small hex socket set screws when the adjustment is complete just until the screws lightly touch the rods to avoid damaging the rod threads 7007452 CryoMed Freezer 2 21 Thermo Scientific Section 2 Set Up and Operation Holder Adjustment Figure 2 7 Vial...

Page 35: ......

Page 36: ...s Code Chart Recorder Printer Probe Calibration Service Manual Control Print Error Logs Reset Error Logs Simulation Software Checksum Test Ram Thermal Controller Use Factory Defaults Time and Date Tracking Alarms 1 Configure Calibrate A Change Access Code Change the four digit security access code by selecting each digit using the Enter and Back keys and changing the digit using the up down arrow ...

Page 37: ... the pen to the graph paper 3 From the freezer Configure calibrate menu select Chart Recorder Turn the chart recorder on to Scan mode and press the Enter key 4 Set the temperature to 0 C zero IMPORTANT If the temperature is already set to 0 C move it up or down 10 C then back to 0 C The recorder pen should move to 0 C If it does not adjust it using the zero knob on the recorder until the pen reach...

Page 38: ...ibration Allows operator to calibrate the Chamber or Sample probes by entering an offset and scale factor scaler Exit this menu by using the Back key Use an appropriate reference thermometer for comparison near 0 C offset and 196 C scaler Note The chamber sample limit temperature alarm will sound during the scale factor adjustment at 196 C Silence the alarm during the calibration procedure by pres...

Page 39: ...he center of the container well away from the container walls 4 Observe the Chamber and Sample temperature displays Adjust the Chamber and Sample Offset parameters so that the displays each read 0 0 C Move the cursor to the proper parameter by using the Enter Back keys and change the parameter using the up down arrow keys Each degree or tenth of a degree of Offset will result in a respective chang...

Page 40: ...ter is energized The solenoid closest to the front of the unit will be energized when Cool is activated B Print Error Logs This menu item prints and resets the diagnostic error logs that are stored in battery RAM Each error log consists of the alarm message and the time date of occurrence A maximum of 20 alarms for each alarm type are stored in the log Older entries are discarded when more than 20...

Page 41: ... power on display E Software Checksum This menu item initiates a checksum calculation of the software in controller memory and compares it to the reference value loaded in the controller when the software was installed In addition the checksum value is shown in the lower right corner of the display F Test RAM This menu item initiates a non destructive test of all RAM on the controller board S RAM ...

Page 42: ...to 0 99 default 0 01 or 50mS minimum on time CP Cool differential temperature adjusted over a range of 1 C to 99 C default 4 C CM Cool multiplier determines the cooling proportional and differential gain constants relative to the constants entered for P and D i e P cool P CM with a range of CM from 0 1 to 9 9 default 1 0 H Use Factory Defaults This menu item restores the factory set parameters for...

Page 43: ...ct Nov Dec Date 01 31 Year 2000 2098 Hour 12 hour format 1 12 Minute 00 59 Second 00 59 AM PM E Tracking Alarms Adjust the High and Low temperature tracking alarms for duration of run Time 1 minute increments and temperature difference from setpoint Delta 1 C increments The Time parameter can be adjusted over a range from a 1 to 99 minute range Exit the menu using the Back key 3 8 7007452 CryoMed ...

Page 44: ...is free from open flame If any component is cleaned with alcohol do not expose the component to open flame or other possible hazard Allow the alcohol to fully dry before turning power on Use an appropriate disinfectant All articles and surfaces to be disinfected must be thoroughly cleaned rinsed and roughdried 1 Turn the freezer off and disconnect the plug from the power source 2 Unplug and remove...

Page 45: ...te disinfectant Clean rinse and wipe dry as needed Clean the screened side ducts and center ducts before reinstalling 7 Press the top and bottom edges of the center duct toward each other and insert the duct into the center of the chamber The edges fit inside the door gasket 8 Install each screened side duct Locate the tabs on the back of each duct into the proper holes at the back of the chamber ...

Page 46: ...nt dissolved in water Dry with a soft cloth Note On IVF models be sure to clean between the sample access openings on the top of the unit Dry any moisture from the door gasket and heat breaks between each run using a soft cloth to prevent ice build up which could reduce the effectiveness of the door seal Clean the Cabinet Exterior Clean Gasket Heat Break ...

Page 47: ......

Page 48: ...arameters which may be set the printer manufacturer default settings and the settings required by the Controlled Rate Freezer See the included printer manual for the software switch setting procedure 1 Turn the unit on 2 Trim the paper as needed to make edge straight paper 3 Open the printer cover 4 Feed the paper into the inlet at the bottom of the paper holder until the auto loader begins to pul...

Page 49: ...sity c ON ON DIP SW 2 switch setting switch setting 1 Print mode 40 columns ON 40 columns ON 2 User font back up Enabled ON Enabled ON 3 Character type Normal ON Normal ON 4 Zero style Normal ON Normal ON 5 Character set a Japanese ON USA ON 6 Character set b ON ON 7 Character set c ON ON 8 Character set d ON OFF DIP SW 3 switch setting switch setting 1 Data length 8 Bits ON 8 Bits ON 2 Parity wit...

Page 50: ...arately for each cabinet size Please note that there are 5 each vials in the second rack from the top as well as the second rack up from the bottom with the Sample vial placed in the center of the top rack Also note that the large cabinet uses a combination of the small and mid size rack and holder sets Finally a specific program sequence listed below must be run in order to collect the uniformity...

Page 51: ... pressure unit size and voltage Chamber Warming Rate Minimum of 0 1 C min no load T couple in free air center of chamber Maximum of 12 0 C min same conditions as above Average of 9 0 C min same conditions as above range 40 C to 12 C Note Rates vary based on load LN2 pressure unit size and voltage 6 2 7007452 CryoMed Freezer Thermo Scientific Section 6 Specifications Weight Model lbs kg 7450 140 63...

Page 52: ...rational Features Door interlock switch turns off LN2 heater and fan motor except during the warm up cycle During the warm up cycle the door can be opened with heater and fan running up to 0 C to accelerate warming LN2 pressure relief valve located adjacent to LN2 connection Chamber pressure vent port located on left side wall Heater overtemperature protection At a temperature level of 93 C a manu...

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Page 54: ...8A DPDT Circuit Breaker Switch 251008 1 4 MPT Relief Valve 60 PSIG 290170 T Type Chamber Probe 370248 T C Plug Type T 370249 T C Socket Type T 4000385 Internal Sample Probe 1 2 ml 4000386 Internal Sample Probe 4 5 ml Part Number Description 4000384 Internal Straw Probe 400152 Switcher 40W 12 5 12 V 400148 200 F Manual Reset T Stat 430326 Cordset 13A 125V 16 3 USA 430109 Cordset 10A 250V European 4...

Page 55: ... 4000710 Straw Holder 4000711 Cane Holder 180254 Cane Holder Plug 180255 Access Port Plug 4000211 Cane 4000211 Cane 1 2 ml 6 ampule 180254 Cane Holder Plug 4000402 External Straw Sample Probe 4000403 External Vial Sample Probe 4000712 Sample T C Vial with hole 1 2 ml 4000713 Sample T C Vial with hole 2 0 ml ...

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Page 78: ...ppear normal verify adjustment of R8 LCD contrast 2 If 5V DC less than 4 75V when power harness connected to controller board 3 LED s and temperature display appear normal and Pre set profile 1 starts when the Run key is pressed 4 Multiple LED s lit Idle Run Warm and or unchanging misc numbers in temperature displays 1 Adjust R8 for optimum contrast 2 Replace power supply may be controller board i...

Page 79: ...nk regulator 3 Replace upgrade hose plumbing 4 Replace solenoids 5 Replace controller board Fan will not run 1 Fuse on controller board 2 Thermal cut out in motor motor overheating 3 Controller board 1 Check fuse value 1a Check for unobstructed rotation of fan 1b Check for free rotation of motor shaft 1c Check for excessive motor current vs name plate 120 230VAC strapping incorrect If not 1d Check...

Page 80: ...e controller board ILL on temperature display 1 Chamber or Sample probe connector screw connector 2 Power supply 3 Controller board 1 Measure resistance between pins on probe harness in sidecar for Chamber or Sample If more than a few ohms 2 Verify 5 12 12VDC at controller board power connector If not 3 Short thermocouple inputs on controller board with jumper wire or bent paper clip If ILL displa...

Page 81: ...ne button on printer 1a Replace thermal paper 1b Clear jam or replace printer 2 Select Chamber Sample or Scan 3 Turn power switch on toward rear of machine 3a Replace printer power supply 4 Reset printer DIP switches No recorder output 1 Recorder power 2 Recorder menu setup 3 Recorder cable connection 4 Recorder 5 Controller board 1 Confirm recorder power light lighted 2 Check Configuration Calibr...

Page 82: ...y tank pressure regulator 22 nominal 2 Verify thermocouple head out of mounting tube inch 3 Verify correct PID values in Service Thermal Controller menu 4 Test for short and verify position 1 Replace tank regulator 2 Adjust thermocouple head position 3 Set correct PID values or reset to factory defaults in the Service menu Make note of probe calibration values prior to reset to factory defaults or...

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Page 84: ...software communicates with the CRF through the RS 232 serial connection included with the freezer is a 10 male female DB9 cable and a DB25F to DB9M adapter Data information is routed with a check byte for transmission integrity The software stays in continual touch with the freezer to detect any communication breakdown A single PC can communicate with up to four Controlled Rate Freezers using sepa...

Page 85: ...nd add user names and passwords if password protection of all Main Menu features is desired System software Lab View Run Time Engine is installed along with the PC interface software Note This system software is required for proper operation of the PC interface with the freezer As the PC interface program begins a splash screen Figure 10 1 appears just before the registration screen The splash scr...

Page 86: ... the Edit window graph These are the profile steps available in the pull down menu with definitions following Step Name with Menu Format Step Parameters with Definitions Wait Wait At X X C where X X is the chamber set temperature Maintains specified chamber temperature indefinitely until the user clicks the Program waiting for user Click here to continue button on the Run screen The temperature ca...

Page 87: ...p and Y Y is the Sample trip temp The default Sample trip temp is1 C above Chamber temp Step maintains specified Chamber temp and moves to next step in profile when Sample temp cools to the specified temp This step can only be used when the starting Sample temp is greater than the specified Sample temp the Sample is cooling Note The sample temp must be greater than the chamber temp Edit Screen con...

Page 88: ... rate C minute The rate can be set over a range of 0 1 C minute to 99 9 C minute The temp can be set over a range of 180 C to 50ºC 0 1ºC When the Chamber reaches specified temp the control moves to the next step in the profile If Sample sensor is selected Chamber temp changes at a specified rate until Sample temp reaches specified temperature Note The slope of the temperature ramp is determined au...

Page 89: ...ed hold duration before proceeding to the next step The time can be set over a range of 1 to 99 minutes and the temperature can be set over a range of 180 C to 50 C with a setting resolution of 0 1 C Jump JUMP TO STEP X where X is the step number Automatic advance to a subsequent step or return to a previous step in a freezing profile The Jump function can be programmed over a range of Step 1 to S...

Page 90: ...tton or from the freezer keypad Follow the procedure below to create a new freezing profile 1 Select the desired first step type from the Step pull down menu WAIT or WAIT FOR TEMPERATURE is the mandatory first step It is required to purge the warm LN2 plumbing prior to initiating controlled rate freezing This step cannot be deleted or changed to a different step type 2 Select subsequent steps Use ...

Page 91: ... window 5 Subsequent steps move down one position as new ones are added 6 Select the step type and enter the desired parameters 7 Press the Insert button to insert the new step Use the Replace button to modify or edit a step Use the Delete button to delete a step or the Delete All button to delete all the steps in the profile 8 Save the edited profile The same file name may be used or a new profil...

Page 92: ...vertically by using the scroll buttons Scale adjustments can be made using the time and temperature pull down menus If selected step parameters are out of range an error message will appear when the Enter or Replace button is pressed Press OK and correct the parameters When saving a profile which has the same name as one already existing a file overwrite error message will appear Choose YES to ove...

Page 93: ...start of a run the bar shows the communication port the profile name and the run data storage file name The Start button initiates a Run This button changes to Move to during a Run when a different step is selected in the Step pull down menu The Print button initiates a print out during a Run or after a Run is complete using the Windows default printer Color or monochrome output may be selected a ...

Page 94: ...ts the Run screen after a Run has ended The Run screen may be exited during Warm However the Controlled Rate Freezer will maintain Warm Mode until terminated by the user at the keypad Auto Scroll button is an On Off button with the default being On This button has an automatic adjustment of the graph scale so that it is always visible in the window In addition the most recent data is automatically...

Page 95: ...hosen step Chamber Sample temperature window indicates these temperatures at a chosen point on the graph To choose a point on the graph first place the mouse pointer on the default cursor position left side of the on screen graph yellow vertical line Press and hold the left mouse button and drag the curser to the desired point on the graph Graph legend selection box includes the legend which shows...

Page 96: ...ndicators show the present temperatures at the freezer ILL Indicates an open Sample or Chamber thermocouple probe The Operating Status indicators are as follows Idle CRF is in idle mode fan solenoids and heater turned off Run Running a profile Wait Running a profile but waiting for user intervention End Running a profile but at the End last step in the profile Warm Warming the chamber to 25C Pop u...

Page 97: ...Click Here To Silence Click anywhere on the alarm window to silence the audible alarm at the freezer After the audible alarm is silenced another alarm window appears showing the list of up to three most recent alarm and the message Click Here To Clear Click anywhere on the alarm window to clear all alarms whose conditions have been resolved The alarm window reappears if any alarms are still active...

Page 98: ...rs are used in the data tag the program runs normally but no data file is created 5 Enter any optional data in previously enabled Information Fields Up to 12 fields can be enabled and named in the Options screen The information entered is captured in the Run file and displayed on the Review screen and information page print outs 6 Select the starting step of the profile Default is the first step i...

Page 99: ...the PC continues to operate When power is restored the freezer will continue the Run at the point of failure However the freezer runs in Stand alone mode All commands from the PC are ignored except for profile status requests The temperature data is continually captured and plotted The PC cannot clear alarms or stop the Run The freezer s keypad remains disabled except for the Silence key which may...

Page 100: ...e Up to four Run screens can be restored for freezers that were running during the PC power failure As the freezer did not experience the power failure no alarm will be recorded If both the freezer and the PC have power failures see the previous paragraph The behavior and recovery are the same as above Communication Failure CRF Not Responding This message indicates that the freezer is not sending ...

Page 101: ...Time and temperature details are captured every 6 seconds The CRF keypad is disabled except for the Silence key which can be used to silence the audible alarm and clear alarm messages at the freezer Alarm messages on the PC must be cleared at the PC as an added assurance that the PC user will be aware of the alarm condition The other freezer keypad keys are disabled to prevent persons near the fre...

Page 102: ...ny alarm messages Any attempt to open a Run screen for a freezer already running a local profile will result in an error message Freezer Already In Use The Run screen cannot be opened Refer to the details below concerning the information fields and user controls for the review screen Data Tag Shows mandatory information entered by the user for the open Run file Date Time Shows the start time date ...

Page 103: ...s a second file for comparison Both files are plotted on the graph Click the Compare button again to remove the comparison data Print Button This button is used to initiate a print out of the Review and Compare data using the Windows default printer Select color or monochrome black white output If the printer is monochrome select monochrome If color select either Monochrome uses marker line patter...

Page 104: ...ofile listing 8 Alarm listing with time of occurrence If a Compare file is open a second information sheet is printed Multi page Report prints information pages on the Review profile and the Compare profile if open Subsequent pages are the entire Run graph Horizontal scale is as selected on the Review screen This also determines the number of pages as indicated in the print out selection window Ve...

Page 105: ...erature at a selected point on the graph The default cursor position is the left side of the on screen graph yellow vertical line Point at cursor press and hold the left mouse button and drag the cursor to the desired graph position Graph color selection box This box includes a graph legend showing the colors plot styles of on screen plot lines Select from a variety of colors styles for the curren...

Page 106: ...oose plot colors for the Chamber temp Sample temp Profile Alarm steps background and grid Save before leaving the Options screen To choose colors click on the color box corresponding to the desired plot item Move the cursor over the desired color observe the changes in the sample graph Click the palette button to create custom colors When the cursor is over the desired color click the left mouse b...

Page 107: ... for each enabled information field Click Save to keep the changes Click Restore to return to previously saved changes Click Exit to exit the Options screen Directories may be set up to store program profiles and run files Save before leaving the Default Directories screen Enter the desired path in the window for the default program directory and run data directory A Windows directory browse butto...

Page 108: ...er names 6 To change a password select the user name from the pull down list or type the name in User Name window enter the new password and click the Modify button 7 To delete a user name select the user name form the pull down list or type name in User Name window and click the Delete button 8 Click Save to keep changes 9 Click Restore to return to previously saved changes 10 Click Exit to exit ...

Page 109: ...the serial communication interruption Experiment with test runs or if in doubt don t print during a critical run Using other applications on the same PC with a Run in progress may cause the loss of Run data points Serial port communication may stop for a short time if PC resources are consumed by other applications Chart lines continue across the communication gaps Slow ramps or holds will lose ve...

Page 110: ...ction manuals carefully detail equipment installation operation and preven tive maintenance Thermo shall not be liable for any indirect or consequential damages including without limitation damages relating to lost profits or loss of products If equipment service is required please call your Technical Services Department at 1 888 213 1790 USA and Canada or 1 740 373 4763 We re ready to answer your...

Page 111: ...l not be liable for any indirect or consequential damages including without limitation damages relating to lost profits or loss of products Your local Thermo Sales Office is ready to help with comprehensive site preparation information before your equipment arrives Printed instruction manuals carefully detail equipment installation operation and preventive maintenance If equipment service is requi...

Page 112: ...er the equipment or to the component part beyond the original warranty period The Technical Services Department must give prior approval for return of any components or equipment At Thermo s option all defective parts must be returned to Thermo postage paid and replacement parts are shipped FOB destination THIS WARRANTY IS EXCLUSIVE AND IN LIEU OF ALL OTHER WARRANTIES WHETHER WRITTEN ORAL OR IMPLI...

Page 113: ...or repair of components parts or equipment under this warranty shall not extend the warranty to either the equipment or to the component part beyond the original warranty period The Technical Services Department must give prior approval for return of any components or equipment At Thermo s option all defective parts must be returned to Thermo postage paid and replacement parts are shipped FOB dest...

Page 114: ...7007452 CryoMed Freezer AA 1 Thermo Scientific ...

Page 115: ...AA 2 7007452 CryoMed Freezer Thermo Scientific ...

Page 116: ...7007452 CryoMed Freezer AA 3 Thermo Scientific ...

Page 117: ...AA 4 7007452 CryoMed Freezer Thermo Scientific ...

Page 118: ...sed The gloves should fit loosely so that they can be thrown off quickly if liquid should splash into them When handling liquid in open containers it is advisable to wear high top shoes Trousers which should be cuffless if possible should be worn outside the shoes The safe handling and use of liquid nitrogen in cryogenic refrigerators and dewar flasks is largely a matter of knowing the potential h...

Page 119: ...ver is placed in the open ing Never use hollow rods or tubes as dipsticks When a warm tube is inserted into liquid nitrogen liquid will spout from the top of the tube due to gasification and rapid expansion of liquid inside the tube Warning Nitrogen gas can cause suffocation without warning Store and use liquid nitrogen only in a well ventilated place As the liquid evaporates the resulting gas ten...

Page 120: ...Thermo Scientific Controlled Environment Equipment 401 Millcreek Road Marietta Ohio 45750 United States www thermo com ...

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