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ProFlex

 PCR System Installation and Operation

Catalog Numbers  

4483636, 4483637, 4483638, 4484071, 4484073, 4484074, 4484075, 4484076, 4484078

Pub. No.  

 4483807   

Rev.  

 G

Note: 

For safety and biohazard guidelines, see the “Safety”

appendix in the 

ProFlex

 PCR System User Guide

 (Pub. No.

MAN0007697). Read the Safety Data Sheets (SDSs) and follow
the handling instructions. Wear appropriate protective eyewear,
clothing, and gloves.

Product description

This document summarizes procedures for installing and using

the ProFlex

Thermal Cycler, with "better than gradient

" VeriFlex

technology. For detailed instructions, see the 

ProFlex

 PCR

System User Guide

 (Pub. No. MAN0007697).

Figure 1   ProFlex

 3 × 32

Well PCR System

Required materials not provided

• Can of compressed air (For use in cleaning wells of sample

block).

(Optional)

 Electrical protective devices.

Note: 

The use of one or more of the following electrical

protective devices is recommended.

·

Power line regulator (1.5 kVA)

·

Surge protector/line conditioner (10-kVA)

·

Uninterruptible power supply (1.5-kVA)

Set up the ProFlex

Thermal Cycler

IMPORTANT! 

Save the packing materials and box in case you

need to ship the instrument in for service.

1.

Open the shipping crate to unpack the ProFlex

Thermal

Cycler. The instrument is contained in three boxes; one with

the base module, a second with the sample block, and the

third containing accessories.

2.

Remove the packaging material, then inspect the instrument

for shipping damage.

Figure 2   ProFlex

 Base module packaging

Figure 3   ProFlex

 3x32-Well Sample Block and

accessories packaging

3.

Use compressed air to clear out each well of the sample

block to remove particles that may have collected inside

during shipping.

4.

Place the instrument at an installation site that:

• Meets the spatial and weight requirements for the

thermal cycler (see “Technical specifications” on

page 7).

• Is within 1 m (3 ft) of a 800-VA power receptacle.

QUICK REFERENCE

For Research Use Only. Not for use in diagnostic procedures.

Summary of Contents for 4483636

Page 1: ...protective devices is recommended Power line regulator 1 5 kVA Surge protector line conditioner 10 kVA Uninterruptible power supply 1 5 kVA Set up the ProFlex Thermal Cycler IMPORTANT Save the packing materials and box in case you need to ship the instrument in for service 1 Open the shipping crate to unpack the ProFlex Thermal Cycler The instrument is contained in three boxes one with the base mo...

Page 2: ...ent Ethernet port using a cable Connect via wireless connection with the USB enabled Wi Fi Module Cat No A26774 Insert the Wi Fi Module into the USB port at the back of the instrument For instructions on setting up the wireless connection see How to set up the wireless connection for the ProFlex Thermal Cycler on page 3 1 USB WiFi port 2 Ethernet port 10 Press the power button then wait for the in...

Page 3: ...k Sign up now and follow the prompts to create an account Link the PCR instrument to Connect Administrator only The procedure to link the Administrator profile to Connect only needs to be performed the first time the PCR instrument is added to Connect 1 Press Sign In Sign In then select the Administrator profile 2 Log in The default password for the Administrator profile is password last four digi...

Page 4: ... heated cover can be as high as 110 C and the temperature of the sample block s can be as high as 100 C Before performing the procedure keep hands away until the heated cover and sample block s reach room temperature To ensure proper operation Regularly Wipe the instrument surfaces with a lint free cloth Clean the vents touchscreen and sample block of the instrument Clean the sample wells with 100...

Page 5: ...croAmp single tubes or tube strips with separate cap strips The blue tray by itself is compatible for use with MicroAmp tube strips with attached caps The black retainer is compatible for use with single MicroAmp reaction tubes with attached caps Prepare samples using MicroAmp tubes tube strips with separate cap strips 1 Separate the blue tray and retainer by squeezing the release catch as indicat...

Page 6: ... downward pressure on the sealing tool in all steps to form a complete seal on top of the tubes 1 Align and place the cap strips on the tubes 2 Seal the cap strips using the rocking capping tool a Slip your fingers through the handle with the holes in the tool facing down b Place the holes in the tool over the first eight caps in a row c Rock the tool back and forth a few times to seal the caps d ...

Page 7: ...ure uniformity 0 5 C 20 seconds after reaching 95 C Dimensions Height 27 2 cm 10 7 in Width 33 0 cm 13 in Depth 56 5 cm 22 in Weight 18 8 kg 41 lb 20 4 kg 45 lb PCR volume range 10 80 µL 10 100 µL 33 nL 5 20 µL Instrument memory USB onboard Display interface 8 4 inch color TFT LCD Power 100 240 V 50 60 Hz Max 950 VA Note For Australia AU New Zealand NZ only 230 VAC 50 Hz Max 950 W Max Current 10 A...

Page 8: ...late Optical 384 Well Clear Reaction Plates with Barcode 4483285 4483273 Strip tubes and caps MicroAmp Fast 8 Tube Strip 0 1 mL 4358293 MicroAmp Optical 8 Tube Strip with Attached Optical Caps 0 2 mL A30588 MicroAmp Optical 8 Tube Strip with Attached Domed Caps 0 2 mL A30589 MicroAmp 8 Tube Strip 0 2 mL N8010580 MicroAmp Optical 8 Tube Strip 0 2 mL 4316567 MicroAmp 8 Cap Strip clear N8010535 N8011...

Page 9: ...n this guide is subject to change without notice DISCLAIMER TO THE EXTENT ALLOWED BY LAW THERMO FISHER SCIENTIFIC INC AND OR ITS AFFILIATE S WILL NOT BE LIABLE FOR SPECIAL INCIDENTAL INDIRECT PUNITIVE MULTIPLE OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT INCLUDING YOUR USE OF IT Important Licensing Information These products may be covered by one or more Limited Use La...

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